1887

Microbiology Society logo

Volume 140, Issue 6

Further evidence for the non-clonal population structure of : extensive genetic diversity within isolates of the same electrophoretic type Free

Abstract

Analysis of data from multilocus enzyme electrophoresis has revealed that populations are non-clonal. Fifteen percent of 227 isolates of had an identical multilocus genotype (ET1) and were recovered world-wide over a 26 year period. The recovery of isolates of identical multilocus genotype from geographically and temporally unassociated hosts is a common criterion of a clonal population structure. However, in a recombining (non-clonal) population, isolates with the same multilocus genotype can arise by the random association of the most common alleles in the population. Analysis of the variation in two further enzymes, in the restriction patterns obtained from the glutamine synthetase gene, and in the DNA fragments obtained using an arbitrarily primed polymerase chain reaction, was used to show that members of ET1 were almost as variable as randomly selected isolates. Unlike the situation in a strongly clonal species, the 26 ET1 isolates examined were increasingly sub-divided to give 19 distinguishable groups as variation at further loci was examined, and 24 distinguishable groups when auxotypes were also considered. We conclude that, as expected of a non-clonal population, the most commonly encountered multilocus genotype does not define a clone.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): electrophoretic type , Neisseria gonorrhoeae , non-clonal population structure and recombination
© Society for General Microbiology 1994
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-140-6-1285
1994-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/6/mic-140-6-1285.html?itemId=/content/journal/micro/10.1099/00221287-140-6-1285&mimeType=html&fmt=ahah

References

  1. Arthur M., Arbeit R. D., Cheung K., Beltran P., Crowe H., Steinbach S., Campanelli C., Wilson R. A., Selander R. K., Goldstein R. 1990; Restriction fragment length polymorphisms among uropathogenic Escherichia coli isolates: pap-related sequences compared with rrn operons. Inject Immun 58:471–479
     [Google Scholar]
  2. Chun P. K., Sensabaugh G. F., Vedros N. A. 1985; Genetic relationships among Neisseria species assessed by comparative enzyme electrophoresis. J Gen Microbiol 131:3105–3115
     [Google Scholar]
  3. Denny T. P., Gilmour M. N., Selander R. K. 1988; Genetic diversityrelationships of two pathovars of Pseudomonas syringae. J Gen Microbiol 134:1949–1960
     [Google Scholar]
  4. Eardly B. D., Materon L. A., Smith N. H., Johnson D. A., Rumbaugh M. D., Selander R. K. 1990; Genetic structure of natural populations of the nitrogen-fixing bacterium Rhizpbium meliloti. Appl Environ Microbiol 56:187–194
     [Google Scholar]
  5. Fekete A., Bantle J. A., Hailing S. M., Stich R.W. 1992; Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers. J Bacterial 174:7778–7783
     [Google Scholar]
  6. Gargallo-Viola D. 1989; Enzyme polymorphism, prodigiosin productionplasmid fingerprints in clinicalnaturally occurring plasmids of Serratia marcesens. J Clin Microbiol 27:860–868
     [Google Scholar]
  7. Istock C. A., Duncan K. E., Ferguson N., Zhou X. 1992; Sexuality in a natural population of bacteria - Bacillus subtilis challenges the clonal paradigm. Mol Ecol 1:195–203
     [Google Scholar]
  8. Maynard Smith J., Smith N. H., O'Rourke M., Spratt B. G. 1993; How clonal are bacteria?. Proc Natl Acad Sci USA 90:4384–4388
     [Google Scholar]
  9. Miller R.D., Hartl D. L. 1986; Biotyping confirms a nearly clonal population structure in Escherichia coli. Evolution 40:1–12
     [Google Scholar]
  10. Musser J. M., Bemis D. A., Ishikawa H., Selander R. K. 1987; Clonal diversityhost distribution in Bordetella bronchiseptica. J Bacteriol 169:2793–2803
     [Google Scholar]
  11. Musser J. M., Kroll J. S., Moxon E. R., Selander R. K. 1988; Clonal population structure of encapsulated Haemophilus influenzae. Infect Immun 56:1837–1845
     [Google Scholar]
  12. Ochman H., Whittam T. S., Caugant D. A., Selander R. K. 1983; Enzyme polymorphismgenetic population structure in Escherichia coliShigella. J Gen Microbiol 129:2715–2726
     [Google Scholar]
  13. O'Rourke M., Stevens E. 1993; Genetic structure of Neisseria gonorrhoeae populations: a non-clonal pathogen. J Gen Microbiol 139:2603–2611
     [Google Scholar]
  14. Pinero D., Martinez E., Selander R. K. 1988; Genetic diversityrelationships among isolates of Rhizpbium leguminosarum biovar. phaseoli. Appl Environ Microbiol 54:2825–2832
     [Google Scholar]
  15. Selander R.K., Musser J. M. 1990; Population genetics of bacterial pathogenesis. In Molecular Basis of Bacterial Pathogenesis pp. 11–36 Edited by Iglewski B. H., Clark V. L. . Orlando: Academic Press;
     [Google Scholar]
  16. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. 1986; Methods of multilocus enzyme electrophoresis for bacterial population geneticssystematics. Appl Environ Microbiol 51:873–884
     [Google Scholar]
  17. Selander R. K., Beltran P., Smith N. H., Barker R. M., Crichton P. B., Old D. C., Musser J. M., Whittam T. S. 1990; Genetic population structure, clonal phylogenypathogenicity of Salmonella paratyphi B. Infect Immun 58:2262–2275
     [Google Scholar]
  18. Souza V., Nguyen T. T., Hudson R. R., Pinero D., Lenski R. E. 1992; Hierarchical analysis of linkage disequilibrium in Rhizpbium populations: evidence for sex?. Proc Natl Acad Sci USA 89:8389–8393
     [Google Scholar]
  19. Vazquez J. A., de la Fuente L., Berron S., O'Rourke M., Smith N. H., Zhou J., Spratt B. G. 1993; Ecological separationgenetic isolation of Neisseria gonorrhoeaeNeisseria meningitidis. Curr Biol 3:567–572
     [Google Scholar]
  20. Zhang Q.-Y., Jones D. M., Saez Nieto J. A., Perez Trallero E., Spratt B. G. 1990; Genetic diversity of penicillin binding protein 2 genes of penicillin resistant strains of Neisseria meningitidis revealed by fingerprinting of amplified DNA. Antimicrob Agents Chemother 34:1523–1528
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-140-6-1285
Loading
Further evidence for the non-clonal population structure of Neisseria gonorrhoeae: extensive genetic diversity within isolates of the same electrophoretic type
Microbiology 140, 1285 (1994); https://doi.org/10.1099/00221287-140-6-1285
/content/journal/micro/10.1099/00221287-140-6-1285
/content/journal/micro/10.1099/00221287-140-6-1285
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error