1887

Abstract

Lipopolysaccharides (LPS) from and were analysed for chemical composition. The main sugar of the lipid A fractions was in each case 2,3-diamino-2,3-dideoxy-D-glucose. Lipid A of also contained a substantial amount of D-glucosamine. In each lipid A fraction a complex fatty acid pattern was detected. This comprised at least 19 different 3-hydroxy fatty acids (amide-linked), three 2,3-dihydroxy fatty acids (amide-linked), non-hydroxy fatty acids (ester-linked) as well as long-chain (Ω-1)-oxo, (Ω-1)-hydroxy and (1, Ω)-dioic fatty acids (ester-linked). In addition, and contained α-hydroxylated long-chain (Ω-1)-oxo and (1, Ω)-dioic fatty acids. The polysaccharide parts of and LPS were similar and contained mainly L-rhamnose, L-fucose, D-mannose, D-glucose, L-fucosamine, D-glucosamine, 2-keto-3-deoxy-octonic acid (Kdo) as well as the rare octose yersiniose A. The corresponding composition of LPS was simpler and consisted mainly of L-rhamnose and 3-amino-3,6-dideoxy-D-mannose. LPS of and contained, in addition, 2-keto-octonic acid linked to Kdo. Phosphorylated sugar constituents were detected in all three LPS, whereas ethanolamine was found only in LPS from . The SDS-PAGE band pattern of differed from the two others in a higher proportion of the low molecular mass constituents.

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/content/journal/micro/10.1099/00221287-140-6-1261
1994-06-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-140-6-1261
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