A new non sulphur photosynthetic bacterium, strain NKPB 0021, was isolated from sea water samples and assigned to the genus Rhodobacter on the basis of morphology and presence of vesicular photosynthetic membranes. Cells are motile rods, 0.6–1.2 μm in diameter and multiply by binary fission. This isolate is unusual since it is oxygen sensitive and cannot grow aerobically. Strain NKPB 0021 grows best anaerobically in the light and requires NaCl for growth (optimum 1.5%). Cells are capable of assimilatory sulphate reduction, and are sensitive to sulphide, with no growth above 0.7 mM sulphide. The mean DNA base composition is 66.7 mol% G + C. On the basis of this study, we propose strain NKPB 0021 to be the type strain of a new species, Rhodobacter marinus.
BurgessJ.G.,
SudoH.,
SodeK.,
MatsunagaT. Efficient gene transfer of high copy number mobilizable plasmids containing a marine Rhodobacter origin of replication. Curr Microbiol1993a; 26:105–108
BurgessJ.G.,
SudoH.,
SodeK.,
MatsunagaT. Gene transfer in Chromatiumpurpuratum, a marine sulphur photosynthetic bacterium producing antibiotics. J Mar Biotechnol1993b; 1:101–104
GestH.,
FavingerJ.L.,
MadiganM.T. Exploitation of N2-fixation capacity for enrichment of anoxygenic photosynthetic bacteria in ecological studies. FEMS Microbiol Ecol1985; 31:317–322
JorgensenB.B. Processes at the sediment water-interface. In The Major Biogeochemical Cycles and their Interactions1983 Edited by
BolinB.,
CookR.B.
New York: John Wiley and Sons; pp 477–509
Katayama-FujimuraY.,
KomatsuY.,
KuraishiH.,
KanekoT. Estimation of DNA base composition by high performance liquid chromatography of its nuclease PI hydrolysate. Agric Biol Chem1984; 48:3169–3172
MatsunagaT.,
MatsunagaN.,
TsubakiK.,
TanakaT. Development of a gene cloning system for the hydrogen producing marine photosynthetic bacterium Rhodopseudomonas sp. J Bacteriol1986; 168:460–463
MatsunagaT.,
TsubakiK.,
MiyashitaH.,
BurgessJ.G. Chloramphenicol acetyltransferase expression in marine Rhodobacter sp NKPB0021 by use of shuttle vectors containing the minimal replicon of an endogenous plasmid. Plasmid1990; 24:90–99
NeutzlingO.,
ImhoffJ.F.,
UperH.G. Rhodopseudomonas adriatica sp nov, a new species of the Rhodospirillaceae dependent on reduced sulfur compounds. Arch Microbiol1984; 137:256–261
NiedermanR.A.,
GibsonK.D. Isolation and physicochemical properties of membranes from purple photosynthetic bacteria. In The Photo synthetic Bacteria1978 Edited by
ClaytonR.K.,
SistromW.R.
New York & London: Plenum Press; pp 79–118
RemsenC.C. Comparative subcellular architecture of photosynthetic bacteria. In The Photosynthetic Bacteria1978 Edited by
ClaytonR.K.,
SistromW.R.
New York & London: Plenum Press; pp 31–60
WillisonJ.C. Biochemical genetics revisited: the use of mutants to study carbon and nitrogen metabolism in the photosynthetic bacteria. FEMS Microbiol Rev1993; 104:1–38