1887

Abstract

In contrast to other mycoplasma species the 16S/23S rRNA and 5S rRNA operons of and map at least 150 kb apart (20% of the genome). Both operons from have been cloned and sequenced. The 23S rRNA gene sequence showed 96.7% homology with the corresponding gene of , equalling that found earlier for 16S rRNA and confirming the close phylogenetic relationships of these organisms. A possible upstream promoter was identified. Sequence elements upstream and downstream from each structural gene could form a stem needed for maturation of the immature rRNA transcript to mature 16S and 23S rRNA. We also identified two possible stem-and-loop sequences 3' to the 23S rRNA gene. The 5S rRNA gene itself also showed high homology with the corresponding structural gene of , although the upstream and downstream sequences were highly heterologous.

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/content/journal/micro/10.1099/00221287-140-4-857
1994-04-01
2022-01-19
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References

  1. Friis N.F. Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare. Nord X 'eterinaermed 1975; 27:337–339
    [Google Scholar]
  2. Gross-Bellard M., Oudet P., Chambon P. Isolation of high molecular weight DNA from mammalian cells. Eur J Biochem 1973; 36:32–38
    [Google Scholar]
  3. Harasawa R., Uemori T., Asada K., Kato I., Shiragami N. boxA'-like sequence between the 16S/23S spacer in rRNA operon of mycoplasmas. FEBS Lett 1992; 297:209–211
    [Google Scholar]
  4. Huang Y., Stemke G.W. Construction of the physical maps of Mycoplasma hyopneumoniae and Mycoplasma flocculare and the location of rRNA genes on these maps. Can J Microbiol 1992; 38:659–663
    [Google Scholar]
  5. Ludwig W., Kirchhof G., Klugbauer N., Weizenegger M., Bextzl D., Ehrmann M., Hertel C., Jilg S., Tatzel R., Zitzelsberger H., Liebl S., Hochberger M., Shah J., Lane D., Wallnofer P.R., Scheifer K.H. Complete 23S ribosomal RNA sequences of Gram-positive bacteria with a low DNA G + C content. Appl Microbiol 1992; 15:487–501
    [Google Scholar]
  6. Olsen G.J., Woese C.R. Ribosomal RNA-a key to phylogeny. F ASEB J 1993; 7:113–123
    [Google Scholar]
  7. Stemke G.W., Laigret F., Grau O., Bov J.M. Phylogenetic relationships of three porcine mycoplasmas, Mycoplasma hyopneumoniae, Mycoplasma flocculare, and Mycoplasma hyorhinis, and complete 16S rRNA sequence of M. flocculare. Int J Syst Bacteriol 1992; 42:220–225
    [Google Scholar]
  8. Taschke C., Herrmann R. Analysis of transcription and processing signals of the 16S-23S rRNA operon of Mycoplasma hyopneumoniae. Mol & Gen Genet 1986; 205:434–441
    [Google Scholar]
  9. Taschke C., Klinkert M.Q., Wolters J., Herrmann R. Organization of the ribosomal RNA genes in Mycoplasma hyopneumoniae: the 5S rRNA gene is separated from the 16S and 23S rRNA genes. Mol & Gen Genet 1986; 205:428–433
    [Google Scholar]
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