1887

Abstract

In the recently described species , two sizes of PCR amplicon were detected with primers homologous to conserved regions of the 16S rRNA gene. A conventionally sized gene was sequenced from the type strain, NCTC 12470, placing the new species as phylogenetically related to and the thermotolerant campylobacters. This nucleotide sequence enabled PCR primers to be designed for use in rapid molecular identification of and its closest phylogenetic relative, . When this assay was employed to characterize 22 ‘-like’ isolates, twelve were identified as and nine as , in agreement with data obtained with a -specific DNA probe. A 550 bp amplicon internal to the 16S rRNA gene of was used to determine restriction fragment length polymorphisms (RFLPs) in genomic Southern blots, confirming that the copy number of the gene was three, and identifying nine 16S rRNA gene profiles. In 5/12 isolates identified by PCR, an enlarged amplicon was detected. The enlarged 16S rRNA gene of one of these strains, NCTC 12838, was sequenced and shown to contain an atypical intervening sequence (IVS) of 148 nucleotides. The position and size of such an IVS was inferred in the other four isolates by PCR with primers 5′ and 3′ to its position in NCTC 12838. This is a first report of an IVS in the 16S rRNA gene of a eubacterium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-140-4-847
1994-04-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/4/mic-140-4-847.html?itemId=/content/journal/micro/10.1099/00221287-140-4-847&mimeType=html&fmt=ahah

References

  1. Altwegg M., Hickman-Brenner F.W., Farmer J.J.I. Ribosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains. J Infect Dis 1989; 160:145–149
    [Google Scholar]
  2. Brosius J., Palmer M.L., Kennedy P.J., Noller H.F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 1978; 75:4801–4805
    [Google Scholar]
  3. Burggraf S., Larsen N., Woese C.R., Stetter K.O. An intron within the 16S ribosomal RNA gene of the archaeon Pyrobaculum aerophilum. Proc Natl Acad Sci USA 1993; 90:2547–2550
    [Google Scholar]
  4. Burgin A.B., Parodos K., Lane D.J., Pace N.R. The excision of intervening sequences from Salmonella 23S ribosomal RNA. Cell 1990; 60:405–414
    [Google Scholar]
  5. Burnens A.P., Nicolet J. Detection of Campylobacter upsaliensis in diarrheic dogs and cats, using a selective medium with cefoperazone. Am J Net Res 1992; 53:48–51
    [Google Scholar]
  6. Fox J.G., Maxwell K.O., Taylor N.S., Runsick C.D., Edmonds P., Brenner D.J. “Campylobacter upsaliensis” isolated from cats as identified by DNA relatedness and biochemical features. J Clin Microbiol 1989; 10:2376–2378
    [Google Scholar]
  7. Giesendorf B.A.J., Quint W.G.V., Henkens M.H.C., Stege-man H., Huf F.A., Niesters H.G.M. Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction. Appl Environ Microbiol 1992; 58:3804–3808
    [Google Scholar]
  8. Goossens G., Vlaes L., De Boeck M., Pot B., Kersters K., Levy J., De Mol P., Butzler J.-P., Vandamme P. Is “Campylobacter upsaliensis” an unrecognised cause of human diarrhoea. Lancet 1990; 335:584–586
    [Google Scholar]
  9. Grimont F., Grimont P.A.D. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol 1986; 137B:165–175
    [Google Scholar]
  10. Jukes T.H., Cantor C.R. Evolution of protein molecules. In Mammalian Protein Metabolism 1991 Edited by Munro H.N. New York: Academic Press; 3 pp 21–132
    [Google Scholar]
  11. Kim N.W., Bingham H., Khawaja R., Louie H., Hani E., Neote K., Chan V.L. Physical map of Campylobacter jejuni TGH9011 and localization of 10 genetic markers by use of pulsed-field gel electrophoresis. J Bacteriol 1992; 174:3494–3498
    [Google Scholar]
  12. Lastovica A.J., Le Roux E., Penner J.L. “Campylobacter upsaliensis" isolated from blood cultures of pediatric patients. J Clin Microbiol 1989; 27:657–659
    [Google Scholar]
  13. Linton D., Moreno M., Owen R.J., Stanley J. 16S rrn gene copy number in Helicobacter pylori and its application to molecular typing. J Appl Bacteriol 1992; 73:501–506
    [Google Scholar]
  14. Paster B.J., Dewhirst F.E. Phylogeny of Campylobacters, Wolinellas, Bacteroides gracilis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int J Syst Bacteriol 1988; 38:56–62
    [Google Scholar]
  15. Patton C.M., Shaffer N., Edmonds P., Barret T.J., Lambert M.A., Perlman D.M., Brenner D.J. Human disease associated with “Campylobacter upsaliensis” (catalase-negative or weakly positive Campylobacter species) in the United States. J Clin Microbiol 1989; 27:66–73
    [Google Scholar]
  16. Saitou N., Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
    [Google Scholar]
  17. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning, a Laboratory Manual 2nd edn 1989 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.;
    [Google Scholar]
  18. Sanstedt K., Ursing J., Walder M. Thermotolerant Campylobacter with no or weak catalase activity isolated from dogs. Curr Microbiol 1983; 8:209–213
    [Google Scholar]
  19. Skurnik M., Toivanen P. Intervening sequences (IVSs) in the 23S ribosomal RNA genes of pathogenic Yersinia enterocolitica strains. The IVSs in Y. enterocolitica and Salmonella typhimurium have a common origin. Mol Microbiol 1991; 5:585–593
    [Google Scholar]
  20. Stanley J., Burnens A.P., Linton D., On S.L.W., Costas M., Owen R.J. Campylobacter helveticus sp. nov. a new thermophilic species from domestic animals: characterization and cloning of a species specific DNA probe. J Gen Microbiol 1992; 138:2293–2303
    [Google Scholar]
  21. Stanley J., Linton D., Burnens A.P., Dewhirst F.E., Owen R.J., Porter A., On S.L.W., Costas M. Helicobacter canis sp. nov. a new species from dogs: an integrated study of phenotype and genotype. J Gen Microbiol 1993; 139:2495–2504
    [Google Scholar]
  22. Taylor D., Hiratsuka K., Mueller L. Isolation and characterization of catalase-negative and catalase-weak strains of Campylobacter species, including “Campylobacter upsaliensis", from humans with gastroenteritis. J Clin Microbiol 1989; 27:2042–2045
    [Google Scholar]
  23. Taylor D.E., Eaton M., Yan W., Chang N. Genome maps of Campylobacter jejuni and Campylobacter coli. J Bacteriol 1992; 174:2332–2337
    [Google Scholar]
  24. Wesley I.V., Wesley R.D., Cardella M., Dewhirst F.E., Paster B.J. Oligodeoxynucleotide probes for Campylobacter fetus and Campylobacter hyointestinalis based on 16S rRNA sequences. J Clin Microbiol 1991; 29:1812–1817
    [Google Scholar]
  25. Woese C.R. Bacterial evolution. Microbiol Rev 1987; 51:221–271
    [Google Scholar]
  26. Zuker M., Stiegler P. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Res 1981; 9:133–148
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-140-4-847
Loading
/content/journal/micro/10.1099/00221287-140-4-847
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error