1887

Abstract

Monoclonal antibodies (mAbs) with high specificity for Sp7, and which bind to different antigenic determinants, were characterized using Western blot techniques applied to one- and two-dimensional fingerprints of the outer-membrane components, and by immunogold labelling combined with transmission electron microscopy. One class of mAbs, which bound to Sp7 and the closely related strain Cd, recognized a 100 kDa protein subunit of the polar flagellum. Two classes of strain-specific mAbs for Sp7 bound, respectively, to a 85 kDa outer-membrane protein and to polysaccharide.

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1994-04-01
2021-10-25
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