1887

Abstract

The genetic diversity in isolates from mushroom compost was assessed using various molecular techniques. Restriction fragment length polymorphism (RFLP) analysis of ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) divided the 81 isolates into three major groups, 1, 2 and 3. There was no variation within a group in rDNA, while a low degree of polymorphism was detected in mtDNA. Random amplified polymorphic DNA (RAPD) analysis of 30 randomly chosen isolates, with six primers, in general confirmed the RFLP groups. Nucleotide sequence determination of rDNA internal transcribed spacer (ITS) 1 revealed three distinct ITS types, 1, 2 and 3, possessed by isolates from the respective groups 1, 2 and 3. Based on these molecular data, group 2 isolates, which are aggressive colonizers of mushroom compost, could be clearly distinguished from the isolates belonging to the other two groups.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-140-4-769
1994-04-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/4/mic-140-4-769.html?itemId=/content/journal/micro/10.1099/00221287-140-4-769&mimeType=html&fmt=ahah

References

  1. Bissett J. A revision of the genus Trichoderma. II. Infrageneric classification. Can J Bot 1991; 69:2357–2372
    [Google Scholar]
  2. Braithwaite K.S., Irwin J.A.G., Manners J.M. Restriction fragment length polymorphisms in Colletotrichum gloeo-sporioides infecting Stylosanthes spp. in Australia. Mycol Ret 1990; 94:1129–1137
    [Google Scholar]
  3. Buchko J., Klassen G.R. Detection of length heterogeneity in the ribosomal DNA of Pythium ultimum by amplification of the intergenic region. Curr Genet 1990; 18:203–205
    [Google Scholar]
  4. Chen W., Hoy W., Schneider R.W. Species-specific polymorphisms in transcribed ribosomal DNA of five Pythium species. Exp Mycol 1992; 16:22–34
    [Google Scholar]
  5. Doyle O. Trichoderma green mould-update. Irish Mushroom Rev 1991; 3:13–17
    [Google Scholar]
  6. Fletcher J.T. Trichoderma and PeniciIlium diseases of Agaricus bisporus A literature review for the Horticultural Development Council 1990 London: ADAS;
    [Google Scholar]
  7. Förster H., Kinscherf T.G., Leong S.A., Maxwell D.P. Estimation of relatedness between Phytophthora species by analysis of mitochondrial DNA. Mycologia 1988; 80:466–478
    [Google Scholar]
  8. Förster H., Coffey M.D., Ellwood H., Sogin M.L. Sequence analysis of the small subunit ribosomal RNAs of three zoosporic fungi and implications for fungal evolution. Mycologia 1990; 82:306–312
    [Google Scholar]
  9. Garber R.C., Yoder O.C. Isolation of DNA from filamentous fungi and separation into nuclear, mitochondrial, ribosomal and plasmid components. Anal Biochem 1983; 135:416–422
    [Google Scholar]
  10. Gaudet J., Julien J., Lafay J.F., Brygoo Y. Phylogeny of some Fusarium species, as determined by large-subunit rRNA sequence comparison. Mol Biol Evol 1989; 6:227–242
    [Google Scholar]
  11. Gyllensten U.B., Erlich H.A. Generation of singlestranded DNA by the polymerase chain reaction and its application to direct sequencing of the HEA-DQA locus. Proc Natl Acad Sci USA 1988; 85:7652–7656
    [Google Scholar]
  12. Higgins D.G., Bleasby A.J., Fuchs R. clustal v: improved software for multiple sequence alignment. Comput Appl Biosci 1992; 8:189–191
    [Google Scholar]
  13. Hodson A., Mills P.R., Brown A.E. Ribosomal and mitochondrial DNA polymorphisms in Colletotrichum gloeosporioides isolated from tropical fruits. Mycol Res 1993; 97:329–335
    [Google Scholar]
  14. Hultman T.S., Shl S., Hornes E., Uhlén M. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Rm 1989; 17:4937–4946
    [Google Scholar]
  15. Illingworth C.A., Andrews J.H., Bibeau C., Sogin M.L. Phylogenetic placement of Athelia bombacina, Aureobasidium pullulans and Colletotrichum gloeosporioides inferred from sequence comparisons of small-subunit ribosomal RNAs. Exp Mycol 1991; 15:65–75
    [Google Scholar]
  16. Lee S.B., Taylor J.W. Phylogeny of five fungus-like protoctistan Phytophthora species inferred from the internal transcribed spacers of ribosomal DNA. Mol Biol Evol 1992; 9:636–653
    [Google Scholar]
  17. Levy M., Romao J., Marchetti M.A., Hamer I.E. DNA fingerprinting with a dispersed repeated sequence resolves patho-type diversity in the rice blast fungus. Plant Cell 1991; 3:95–102
    [Google Scholar]
  18. Martin F.N., Kistler H.C. Species-specific banding patterns of restriction endonuclease-digested mitochondrial DNA from the genus Pythium. Exp Mycol 1990; 14:32–46
    [Google Scholar]
  19. Meyer W., Morawetz R., Bbrner T., Kubicek C.P. The use of DNA-fingerprint analysis in the classification of some species of the Trichoderma aggregate. Curr Genet 1992; 21:27–30
    [Google Scholar]
  20. Moody S.F., Tyler B.M. Restriction enzyme analysis of mitochondrial DNA of the Aspergillus flavus group: A. flavus, A. parasiticus and A. nomius. Appl Environ Microbiol 1990a; 56:2441–2452
    [Google Scholar]
  21. Moody S.F., Tyler B.M. Use of nuclear DNA restriction fragment length polymorphisms to analyze the diversity of the Aspergillus flavus group: A flavus, A parasiticus and A nomius. Appl Environ Microbiol 1990b; 56:2453–2461
    [Google Scholar]
  22. Nei M., Li W.H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979; 76:5269–5273
    [Google Scholar]
  23. Nei M., Tajima F. Maximum likelihood estimation of the number of nucleotide substitutions from restriction sites data. Genetics 1983; 105:207–217
    [Google Scholar]
  24. O’Donnell K. Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum (Gibberella pulicaris). Curr Genet 1992; 22:213–220
    [Google Scholar]
  25. Olsen G.J., Lane D.J., Giovannoni S.J., Pace N.R., Stahl D.A. Microbial ecology and evolution: a ribosomal RNA approach. Annu Rev Microbiol 1986; 40:337–365
    [Google Scholar]
  26. Raeder U., Broda P. Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1985; 1:17–20
    [Google Scholar]
  27. Reynolds D.R., Taylor J.W. DNA specimens and the ‘International code of botanical nomenclature’. Taxon 1991; 40:311–315
    [Google Scholar]
  28. Rifai M.A. A revision of the genus Trichoderma. Commonw Mycol Inst Mycol Pap no 1969; 116: pp. 1–56
    [Google Scholar]
  29. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Eaboratory Manual 2nd edn 1989 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  30. Sanger F.S., Nicklen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 1977; 74:5463–5467
    [Google Scholar]
  31. Seaby D.A. Infection of mushroom compost by Trichoderma species. Mushroom J 1987; 179:355–361
    [Google Scholar]
  32. Seaby D.A. Further observations on Trichoderma. Mushroom J 1989; 197:147–151
    [Google Scholar]
  33. Sreenivasa-prasad S., Brown A.E., Mills P.R. DNA sequence variation and interrelationships among Colletotrichum species causing strawberry anthracnose. Physiol Mol Plant Pathol 1992; 41:265–281
    [Google Scholar]
  34. Vaillancourt L.J., Hanau R.M. Genetic and morphological comparisons of Glomerella (Colletotrichum) isolates from maize and from sorghum. Exp Mycol 1992; 16:219–229
    [Google Scholar]
  35. Verbeet M.Ph., Klootwijk J., Heerikhuizen H., Fontijn R., Vreugdenhil E., Planta R.J. Molecular cloning of the rDNA of Saccharomyces rosei and comparison of its transcription initiation region with that of Saccharomyces carlsbergensis. Gene 1983; 23:53–63
    [Google Scholar]
  36. White T.J., Bruns T., Lee S., Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols. A Guide to Methods and Applications 1990 Edited by Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. San Diego: Academic Press; pp 315–322
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-140-4-769
Loading
/content/journal/micro/10.1099/00221287-140-4-769
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error