1887

Abstract

In stress proteins are induced in response to different environmental conditions such as heat shock, salt stress, glucose and oxygen limitation or oxidative stress. These stress proteins have been previously grouped into general stress proteins (Gsps) and heat-specific stress proteins (Hsps). In this investigation the N-terminal sequences of 13 stress proteins of were determined. The quantification of the mRNA and the analysis of the protein synthesis pattern support the initial hypothesis that the chaperones DnaK and GroEL are Hsps in . In contrast, the recently described proteins GsiB, Ctc and RsbW belong to a class of Gsps that are induced by various stresses including heat shock. The main part of the Gsps described in this study failed to be induced in the deletion mutant in response to heat shock. However, all the five Hsps were induced in this mutant in response to heat shock. These data indicate that SigB plays a crucial role in the induction of general stress genes, but is dispensable for the induction of Hsps.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-140-4-741
1994-04-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/4/mic-140-4-741.html?itemId=/content/journal/micro/10.1099/00221287-140-4-741&mimeType=html&fmt=ahah

References

  1. Benson A.K., Haldenwang W.G. Characterization of a regulatory network that controls αB expression in Bacillus subtilis. J Bacteriol 1992; 174:749–757
    [Google Scholar]
  2. Benson A.K., Haldenwang W.G. The αB-dependent promoter of the Bacillus subtilis sigB operon is induced by heat shock. J Bacteriol 1993; 175:1929–1935
    [Google Scholar]
  3. Bhagwat A.A., Apte S.K. Comparative analysis of proteins induced by heat shock, salinity, and osmotic stress in the nitrogen-fixing cyanobacterium Anabaena sp strain L-31. J Bacteriol 1989; 171:5187–5189
    [Google Scholar]
  4. Boylan S.A., Rutherford A., Thomas S.M., Price C.W. Activation of Bacillus subtilis transcription factor SigmaB by a regulatory pathway responsive to stationary-phase signals. J Bacteriol 1992; 174:3695–3706
    [Google Scholar]
  5. Boylan S.A., Redfield A.R., Price C.W. Transcription factor αB of Bacillus subtilis controls a large stationary-phase regulon. J Bacteriol 1993; 175:3957–3963
    [Google Scholar]
  6. Craig E.A., Gross C.A. Is hsp70 the cellular thermometer. Trends Biochem Sci 1991; 16:135–140
    [Google Scholar]
  7. Duncan M.L., Kalman S.S., Thomas S.M., Price C.W. Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal location, and cryptic function. J Bacteriol 1987; 169:771–778
    [Google Scholar]
  8. Haldenwang W.G., Losick R. A modified RNA polymerase transcribes a cloned gene under sporulation control in Bacillus subtilis. Nature 1979; 282:256–260
    [Google Scholar]
  9. Hecker M., Völker U. General stress proteins in Bacillus subtilis. FEMS Microbiol Ecol 1990; 74:197–214
    [Google Scholar]
  10. Hecker M., Heim C., Völker U., Wölfel L. Induction of stress proteins by sodium chloride treatment in Bacillus subtilis. Arch Microbiol 1988; 150:564–566
    [Google Scholar]
  11. Hengge-Aronis R. Survival of hunger and stress-the role of rpoS in early stationary phase gene regulation in E. coli. Cell 1993; 72:165–168
    [Google Scholar]
  12. Igo M., Losick R. Regulation of a promoter that is utilized bv minor forms of RNA polymerase holoenzyme in Bacillus subtilis. J Mol Biol 1986; 191:615–624
    [Google Scholar]
  13. Igo M., Lampe M., Ray C., Schafer W., Moran C.P., Losick R. Genetic studies of a secondary RNA polymerase sigma factor in Bacillus subtilis. J Bacteriol 1987; 169:3464–3469
    [Google Scholar]
  14. Jenkins D.E., Auger E.A., Matin A. Role of RpoH, a heat shock regulator protein, in Escherichia coli carbon starvation protein synthesis and survival. J Bacteriol 1991; 173:1992–1996
    [Google Scholar]
  15. Kalman S., Duncan M.L., Thomas S.M., Price G.W. Similar organization of the sigB and spoIIA operons encoding alternate sigma factors of Bacillus subtilis RNA polymerase. J Bacteriol 1990; 172:5575–5585
    [Google Scholar]
  16. Li M., Wong S.L. Cloning and characterization of the groESL operon from Bacillus subtilis. J Bacteriol 1992; 174:3981–3992
    [Google Scholar]
  17. Lindner G.S., Ulke J., Hecker M. Regulation of xylanolytic enzymes in Bacillus subtilis 1994 Microbiology, in press
    [Google Scholar]
  18. Majumdar D., Avissar Y.J., Wyche J.H. Simultaneous and rapid isolation of bacterial and eucaryotic DNA and RNA: a new approach for isolating DNA. BioTechniques 1991; 11:94–96
    [Google Scholar]
  19. Matin A. The molecular basis of carbon-starvation-induced general resistance in Escherichia coli. Mol Microbiol 1991; 5:3–10
    [Google Scholar]
  20. Meury J., Kohiyama M. Role of heat shock protein DnaK in osmotic adaptation of Escherichia coli. J. Bacteriol 1991; 173:4404–4410
    [Google Scholar]
  21. Miller B.S., Kennedy T.E., Streips U.N. Molecular characterization of specific heat shock proteins in Bacillus subtilis. Curr Microbiol 1991; 22:231–236
    [Google Scholar]
  22. Morita R.Y. Bioavailability of energy and its relationship to growth and starvation survival in nature. Can J Microbiol 1988; 34:436–441
    [Google Scholar]
  23. Mueller J.P., Bukusoglu G., Sonenshein A.L. Transcriptional regulation of Bacillus subtilis glucose starvation-in-ducible genes control of gsiA by the ComP-ComA signal transduction system. J Bacteriol 1992; 174:4361–4373
    [Google Scholar]
  24. Naberhaus F., Bahl H. Cloning, sequencing, and molecular analysis of the groESL operon of Clostridium aceto-butylicum. J Bacteriol 1992; 174:3282–3289
    [Google Scholar]
  25. Neidhardt F.G., Van Bogelen R.A. Heat shock response. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 1987 Edited by Neidhardt F.C., Ingraham J.L., Low K.B., Magasanik B., Schaechter M., Umbarger H.E. Washington, DC: American Society for Microbiology; pp 1334–1345
    [Google Scholar]
  26. Nyström T., Neidhardt F.C. Cloning, mapping and nucleotide sequencing of a gene encoding a universal stress protein in Escherichia coli. Mol Microbiol 1992; 6:3187–3198
    [Google Scholar]
  27. Ray G., Igo M., Shafer W., Losick R., Moran C.P. Suppression of ctc promoter mutations in Bacillus subtilis. J Bacteriol 1988; 170:900–907
    [Google Scholar]
  28. Richter A., Hecker M. Heat-shock proteins in Bacillus subtilis. A two-dimensional electrophoresis study. FEMS Microbiol Eett 1986; 36:69–71
    [Google Scholar]
  29. Roszak D.B., Colwell R.R. Survival strategies of bacteria in natural environments. Microbiol Rep 1987; 51:365–379
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA 1977; 74:5463–5467
    [Google Scholar]
  31. Schmidt A., Schiesswohl M., Völker U., Hecker M., Schumann W. Cloning, sequencing, mapping and transcriptional analysis of the groESL operon from Bacillus subtilis. J Bacteriol 1992; 174:3993–3999
    [Google Scholar]
  32. Schön U., Schumann W. Molecular cloning, sequencing, and transcriptional analysis of the groESL operon from Bacillus stearothermophilus. J Bacteriol 1993; 175:2465–2469
    [Google Scholar]
  33. Segal G., Ron E.Z. Heat shock transcription of the groESL operon of Agrobacterium tumefaciens may involve a hairpin-loop structure. J Bacteriol 1993; 175:3083–3088
    [Google Scholar]
  34. Smith I., Paress P., Cabane K., Dubnau E. Genetics and physiology of the re I system of Bacillus subtilis. Mol & Gen Genet 1980; 178:271–279
    [Google Scholar]
  35. Sonenshein A.L. Metabolic regulation and sporulation and other stationary-phase phenomena. In Regulation of Prokaryotic Development 1989 Edited by Smith I., Slepecky R.A., Setlow P. Washington, DC: American Society for Microbiology; pp 109–130
    [Google Scholar]
  36. Stülke J., Hanschke R., Hecker M. Temporal activation of β-glucanase synthesis in Bacillus subtilis is mediated by the GTP pool. J Gen Microbiol 1993; 139:2041–2045
    [Google Scholar]
  37. Van Bogelen R., Acton M.A., Neidhardt F.C. Induction of the heat shock regulon does not produce thermotolerance in Escherichia coli. Genes & Dev 1987; 1:525–531
    [Google Scholar]
  38. Varon D., Boylan S.A., Okamoto K., Price C.W. Bacillus subtilis gtaB encodes UDP-glucose pyrophosphohydrolase and is controlled by stationary-phase transcription factor αB . J Bacteriol 1993; 175:3964–3971
    [Google Scholar]
  39. Völker U., Mach H., Schmid R., Hecker M. Stress proteins and cross-protection by heat shock and salt stress in Bacillus subtilis. J Gen Microbiol 1992; 138:2125–2135
    [Google Scholar]
  40. Wetzstein M., Völker U., Dedio J., Lttbau S., Zuber U., Schiesswohl M., Herget C., Hecker M., Schumann W. Cloning, sequencing and molecular analysis of the dnaK locus from Bacillus subtilis. J Bacteriol 1992; 174:3300–3310
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-140-4-741
Loading
/content/journal/micro/10.1099/00221287-140-4-741
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error