The operon encodes enzymes for the biosynthesis of uroporphyrinogen III from glutamyl-tRNA. The function of the hemX gene product was studied in this work. The deduced amino acid sequence suggests HemX to be an integral 32 kDa membrane protein. This was confirmed by experiments using minicells and gene fusions. Deletion of the gene from the chromosome demonstrated that this gene is not required for haem synthesis. However, the deletion strain was found to overexpress the gene product, glutamyl-tRNA reductase. A combination of results obtained with and in and shows that HemX negatively affects the steady-state cellular concentration of HemA protein. The mechanism by which HemX affects the HemA concentration is unclear.


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