1887

Abstract

Pediocin A, a bacteriocin produced by FBB61, was purified and partially characterized. The purification was achieved by dialysis against PEG 20000, butanol extraction and electroendosmotic preparative electrophoresis. The protocol led to a 7843-fold increase in the specific activity, with 3·9% activity recovered. SDS-PAGE of pediocin A resulted in a single 80 kDa protein band. The antimicrobial compound was sensitive to proteolytic enzymes and heat (10 min at 100 °C). It exhibited inhibition against species of and .

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/content/journal/micro/10.1099/00221287-140-4-697
1994-04-01
2021-10-20
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References

  1. Barefoot S.F., Klaenhammer T.R. Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B. Antimicrob Agents Chemother 1984; 26:328–334
    [Google Scholar]
  2. Van Belkum M.J., Kok J., Venema G., Holo H., Nes I.F., Konings W.N., Abee T. The bacteriocin lactococcin A specifically increases permeability of lactococcal cytoplasmic membranes in a voltage-independent, protein-mediated manner. J Bacteriol 1991; 173:7934–7941
    [Google Scholar]
  3. Bhunia A.K., Johnson M.C., Ray B. Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Ind Microbiol 1987; 2:319–322
    [Google Scholar]
  4. Bhunia A.K., Johnson M.C., Ray B. Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol 1988; 65:261–268
    [Google Scholar]
  5. Costilow R.N., Coughlin F.M., Robach D.L., Ragheb H.S. A study of the acid-forming bacteria from cucumber fermentations in Michigan. Food Res 1956; 21:27–33
    [Google Scholar]
  6. Curioni A., dal Belin Peruffo A., Nuti M.P. Purification of cellulases from Streptomyces strain A20 by electroendosmotic preparative electrophoresis. Electrophoresis 1988; 9:327–330
    [Google Scholar]
  7. Curioni A., dal Belin Peruffo A., Pogna N.E. Electroendosmotic preparative electrophoresis as a one-step method for purification of high molecular weight subunits of wheat glutenin. Cereal Chem 1989; 66:133–135
    [Google Scholar]
  8. Daeschel M.A., Klaenhammer T.R. Association of a 13·6-megadalton plasmid in Pediococcus pentosaceus with bacteriocin activity. Appl Environ Microbiol 1985; 50:1538–1541
    [Google Scholar]
  9. De Man J.C., Rogosa M., Sharpe M.E. A medium for the cultivation of lactobacilli. J Appl Bacteriol 1960; 23:130–135
    [Google Scholar]
  10. Etchells J.L., Costilow R.N., Anderson T.E., Bell T.A. Pure culture fermentation of brined cucumbers. Appl Microbiol 1964; 12:523–535
    [Google Scholar]
  11. Food and Drug Administration Nisin preparation: affirmation of GRAS status as a direct human food ingredient. Fed Reg April 1988; 6:1988, 53, 11247–11250
    [Google Scholar]
  12. Fleming H.P., Etchells J.L., Costilow R.N. Microbial inhibition by an isolate of Pedicococcus from cucumber brines. Appl Microbiol 1975; 30:1040–1042
    [Google Scholar]
  13. Henderson J.T., Chopko A.L., van Wassenaar P.D. Purification and primary structure of pediocin PA-1 produced by Pediococcus pentosaceus PAC 1.0. Arch Biochem Biophjs 1992; 295:5–12
    [Google Scholar]
  14. Joerger M.C., Klaenhammer T.R. Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J Bacteriol 1986; 167:439–446
    [Google Scholar]
  15. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  16. Motlagh A.M., Bhunia A.K., Szostek F., Hansen T.R., Johnson M.C., Ray B. Nucleotide and aminoacid sequence of PAP-gene pediocin AcH production in Pediococcus acidilactici H. Lett Appl Microbiol 1992; 15:245–48
    [Google Scholar]
  17. Mortvedt C.I., Nissen-Meyer J., Sletten K., Nes I.F. Purification and aminoacid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45. Appl Environ Microbiol 1991; 57:1829–1834
    [Google Scholar]
  18. Muriana P.M., Klaenhammer T.R. Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl Environ Microbiol 1991; 57:114–121
    [Google Scholar]
  19. Piard J.C., Muriana P.M., Desmazeaud M.J., Klaenhammer T.R. Purification and partial characterization of lacticin 481, a lanthionine-containing bacteriocin produced by Lactococcus lactis subs, lactis CNRZ 481. Appl Environ Microbiol 1992; 58:279–284
    [Google Scholar]
  20. Pucci M.J., Vedamuthu E.R., Kunka B.S., Vandenbergh P.A. Inhibition of Listeria monocytogenes by using bacteriocin PA-1 produced by Pediococcus acidilactici PAC 1.0. Appl Environ Microbiol 1988; 54:2349–2353
    [Google Scholar]
  21. Rammelsberg M., Radler F. Antibacterial polypeptides of Lactobacillus species. J Appl Bacteriol 1990; 69:177–184
    [Google Scholar]
  22. Reniero R., Cocconcelli P.S., Bottazzi V., Morelli L. High frequency of conjugation in Lactobacillus mediated by an aggregation-promoting factor. J Gen Microbiol 1992; 138:763–768
    [Google Scholar]
  23. Reniero R., Piva A., Morelli L., Bottazzi V., Cocconcelli P.S. Purification of Lactobacillus secreted proteins. Biotechnol Tech 1993; 7:401–406
    [Google Scholar]
  24. Rueckert P.W. Studies on a bacteriocin-like activity produced by Pediococcus pentosaceus effective against gram-positive organisms 1979 East Lansing: MS thesis, Michigan State University.;
    [Google Scholar]
  25. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Laboratory Manual 2nd edn 1989 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  26. Spelhaug S.R., Harlander S.K. Inhibition of foodborne bacterial pathogens by bacteriocins from Lactococcus lactis and Pediococcus pentosaceus. J Food Prot 1989; 52:856–862
    [Google Scholar]
  27. Wilson K., Goulding K.H. Purificazione degli enzimi. In Biochimica Applicata 1986 Edited by Pilone Simonetta M., Hanozet G. Milan: R. Cortina; pp 90–93
    [Google Scholar]
  28. Yousef A.E., Luchansky J.B., Degnan A.J., Doyle M.P. Behavior of Listeria monocytogenes in weiner exudates in the presence of Pediococcus acidilactici H or pediocin AcH during storage at 4 or 25°C. Appl Environ Microbiol 1991; 57:1461–1467
    [Google Scholar]
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