1887

Microbiology Society logo

Volume 140, Issue 3

A newly isolated lectin from the Plant pathogenic fungus : purification, characterization and role in mycoparasitism Free

Abstract

A novel lectin was isolated and purified from the culture filtrate of the soilborne plant pathogenic fungus by anion-exchange chromatography using a DEAE-Sepharose column. The lectin came through column with the flow-through, whereas all the non-agglutinating proteins present in the crude preparation remained bound to the column until elution in a NaCI gradient. SDS-PAGE analysis of the agglutinating fraction revealed single band corresponding to a protein with a molecular mass of approximately 45 kDa. Agglutination of cells by the purified lectin was not inhibited by any of the mono- or disaccharides tested, wheres the glycoproteins mucin and asialomucin did inhibit agglutination. Protease as well as 1,3--glucanase, were found to be totally destructive to agglutination activity, indicating that both protein and 1,3--glucan are necessary for agglutination. Using a biomimetic system based on binding of the lectin to the surface of inert nylon fibres revealed that the presence of purified agglutinin on the surface of the fibres specifically induced mycoparasitic behaviour in formed tightly adhering coils, which were significantly more frequent with the purified agglutinin-treated fibres than with untreated ones or with those treated with non-agglutinating extracellular proteins from Other mycoparasite-related structures, such as appressorium-like bodies and hyph loops, were only observed in the interaction between and the purified agglutinin-treated fibres.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): clerotium rolfsii , lectin , mycoparasitism and Trichoderma harzianum
© Society for General Microbiology 1994
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-140-3-651
1994-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/3/mic-140-3-651.html?itemId=/content/journal/micro/10.1099/00221287-140-3-651&mimeType=html&fmt=ahah

References

  1. Barak R., Chet I. Lectin of Sclerotium rolfsii: its purification and possible function in fungal interaction. J Appl Bacteriol 1990; 69:101–112
     [Google Scholar]
  2. Barak R., Elad Y., Mirelman D., Chet I. Lectins: a possible basis for specific recognition in Trichoderma-Sclerotium rolfsii interaction. Phytopathology 1985; 75:458–462
     [Google Scholar]
  3. Barak R., Elad Y., Chet I. The properties of L-fucose binding agglutinin associated with the cell wall of Rhizoctonia solani . Arch Microbiol 1986; 144:346–349
     [Google Scholar]
  4. Benhamou N., Chet I. Hyphal interactions between Trichoderma harvfanum and Rhizoctonia solani: ultrastructure and gold cytochemistry of the mycoparasitic process. Phytopathology 1993; 83:1062–1071
     [Google Scholar]
  5. Chet I. Trichoderma — application, mode of action, and potential as biocontrol agent of soilborne plant pathogenic fungi. In Innovative Approaches to Plant Disease Control 1987 Edited by Chet I. New York: John Wiley; pp 137–160
     [Google Scholar]
  6. Chet I. Mycoparasitism - recognition, physiology and ecology. In New Directions in Biological Control: Alternatives for suppressing Agricultural Pests and Diseases 1990 Edited by Baker R., Dunn P. New York: Alan R. Liss; pp 725–783
     [Google Scholar]
  7. Chet I., Harman G. E., Baker R. Trichoderma hamatum: its hyphal interactions with Rhizoctonia solani and Pythium spp. Microb Ecol 1981; 7:29–38
     [Google Scholar]
  8. Dennis C., Webster J. Antagonistic properties of species- groups of Trichoderma. III. Hyphal interaction. Trans Br Mycol Soc 1971; 57:363–369
     [Google Scholar]
  9. Elad Y., Barak R., 8c Chet I. Possible role of lectins in mycoparasitism. J Bacteriol (1983a); 154:1431–1435
     [Google Scholar]
  10. Elad Y., Chet I., Boyle P., 8c Henis Y. Parasitism of Trichoderma spp. on Rhiyoctonia solani and Sclerotium rolfsii- scanning electron microscopy and fluorescence microscopy. Phytopathology 1983b; 73:85–88
     [Google Scholar]
  11. Hamer J. E., Howard R. J., Chumley F. G., Valent B. A mechanism for surface attachment in spores of a plant pathogenic fungus. Science 1988; 239:288–290
     [Google Scholar]
  12. Hoch H. C., 8c Staples R. C. Structural and chemical changes among the rust fungi during appressorium development. Annu Rev Phytopathol 1987; 25:231–247
     [Google Scholar]
  13. Inbar J., 8c Chet I. Biomimics of fungal cell-cell recognition by use of lectin-coated nylon. J Bacteriol 1992; 174:1055–1059
     [Google Scholar]
  14. Kellens J. T., C., Allen A. K., 8c Peumans W. J. Isolation and characterization of lectins from Rhfoctonia crocorum and Athelia rolfsii . J Gen Microbiol 1989; 135:3127–3132
     [Google Scholar]
  15. Kellens J. T., C., Goldstein I. J., Peumans W. J. Lectins in different members of the Sclerotiniaceae. Mycol Res 1992; 96:495–502
     [Google Scholar]
  16. Kritzman G., Chet I., Henis Y. Isolation of extracellular polysaccharides from Sclerotium rolfsii . Can J Microbiol 1979; 57:1855–1859
     [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
     [Google Scholar]
  18. Manocha M. S., Chen Y. Specificity of attachment of fungal parasites to their hosts. Can J Microbiol 1990; 36:69–76
     [Google Scholar]
  19. Nordbring- Hertz B., Mattiasson B. Action of a nematode-trapping fungus shows lectin-mediated host-micro- organism interaction. Nature 1979; 281:477–479
     [Google Scholar]
  20. Nordbring- Hertz B., Chet I. Fungal lectins and agglutinins. In Microbial Lectins and Agglutinins: Properties and Biological Activity 1986 Edited by Mirelman D. New York: John Wiley; pp 393–408
     [Google Scholar]
  21. Okon Y., Chet I., Henis Y. Effect of lactose, ethanol and cycloheximide on translation pattern of radioactive compounds and on sclerotium formation in Sclerotium rolfsii . J Gen Microbiol 1973; 74:151–158
     [Google Scholar]
  22. Sivan A., Chet I. Microbial control of plant diseases. In New Concepts in environmental Microbiology 1992 Edited by Mitchell R. New York: Wiley-Liss; pp 335–354
     [Google Scholar]
  23. Tunlid A., Johansson T., Nordbring-Hertz B. Surface polymers of the nematode-trapping fungus Arthrobotrys oligospora . J Gen Microbiol 1991; 137:1231–1241
     [Google Scholar]
  24. Tunlid A., Jansson H. B., Nordbring-Hertz B. Fungal attachment to nematodes. Mycol Res 96 1992; italic> :401–412
     [Google Scholar]
  25. Vranken A. M., Van Damme E. J. M., Allen A. K., Peumans W. J. Purification and characterization of an N-acetyl- galactosamine specific lectin from the plant pathogenic fungus Rhizoctonia solani . FEBS Lett 1987; 216:67–72
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-140-3-651
Loading
A newly isolated lectin from the Plant pathogenic fungus Sclerotium roltsii: purification, characterization and role in mycoparasitism
Microbiology 140, 651 (1994); https://doi.org/10.1099/00221287-140-3-651
/content/journal/micro/10.1099/00221287-140-3-651
/content/journal/micro/10.1099/00221287-140-3-651
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error