1887

Abstract

Forty strains of and recovered from invasive and non-invasive infections were tested for their susceptibility to complement-mediated lysis by 65% pooled human serum (PHS). Based upon the results of this assay, two major populations could be defined. The first group (= 20) consisted of serogroup 0:11 strains, all of which possessed a paracrystalline surface layer (S layer); all of these strains were refractory to the bactericidal activity of 65% PHS with the exception of strain AH-121, which was composed of mixed subpopulations of serum-susceptible and serum-resistant clones. A second collection of isolates (= 20), all of which were S-layer-negative, contained a subgroup of strains (= 7) that were highly susceptible to complement-mediated lysis, showing a greater than 100-fold reduction of viable progeny within 30 min of exposure to 65% PHS. Serum-resistant strains from both groups could not be lysed by exposure of bacterial cells to polyclonal somatic or whole cell antisera or to 30 μg mlof polymyxin B nonapeptide prior to challenge with 65% PHS. Analysis of selected serum-resistant and serum-susceptible strains from both groups showed that all isolates activated the complement pathway and most bound C3b to the cell surface, indicating that the inability of complement to lyse serum-resistant strains was related to a defect in the terminal portions of the complement pathway. The major differences noticed between serum-resistant and serum-susceptible strains were a lack of a definable lipopolysaccharide side chain profile and higher 50% lethal dose values in strains that were susceptible to complement-mediated lysis.

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1994-10-01
2024-12-03
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References

  1. Abbott S.L., Cheung W. K., Kroske-Bystrom S., Malekzadeh T., Janda J.M. 1992; Identification of Aeromonas strains to genospecies level in the clinical laboratory.. J Clin Microbiol 30:1262–1266
    [Google Scholar]
  2. Aguero M. E., Aron L., DeLuca A. G., Timmis K.N., Cabello F.C. 1984; A plasmid-encoded outer membrane protein, Tra T enhances resistance of Escherichia coli to phagocytosis. Infect Immun 46:740–746
    [Google Scholar]
  3. Akalin H. E., Laleli Y, Telafar H. 1985; Serum bactericidal and opsonic activities in patients with non-alcoholic cirrhosis.. Q J Med 56:431–437
    [Google Scholar]
  4. Blaser M. J., Smith P. F., Hopkins J. A., Heinzer I., Bryner J. H., Wang W.-L. L. . 1987; Pathogenesis of Campylobacter fetus infections: serum resistance associated with high-molecular-weight surface proteins. J Infect Dis 155:696–706
    [Google Scholar]
  5. Brenden R.A., Janda J. M. 1986; The interaction of complement components with Aeromonas species.. Can J Microbiol 32:1–3
    [Google Scholar]
  6. Figueroa J.E., Densen P. 1991; Infectious diseases associated with complement deficiencies. Clin Microbiol Rev 4:359–395
    [Google Scholar]
  7. Hitchcock P. J., Leive L., Makela P. H., Rietschel E. T., Strittmatter W, Morrison D. C. 1986; Lipopolysaccharide nomenclature - past, present, and future. J Bacteriol 166:699–705
    [Google Scholar]
  8. Horwitz M.A., Silverstein S. C. 1980; Influence of Escherichia coli capsule on complement fixation and on phagocytosis and killing by human phagocytes. J Clin Invest 65:82–94
    [Google Scholar]
  9. Janda J.M., Duffey P. S. 1988; Mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum. Rev Infect Dis 10:980–997
    [Google Scholar]
  10. Janda J.M., Kokka R. P. 1991; The pathogenicity of Aeromonas strains relative to genospecies and phenospecies identification. FEMS Microbiol Lett 90:29–34
    [Google Scholar]
  11. Janda J. M., Brenden R., Bottone E. J. 1984; Differential susceptibility to human serum by Aeromonas spp. Curr Microbiol 11:325–328
    [Google Scholar]
  12. Janda J. M., Guthertz L. S., Kokka R. P., Shimada T. 1994; Laboratory characteristics and clinical observations on the role of Aeromonas species in septicemia. Clin Infect Dis 19:77–83
    [Google Scholar]
  13. Kay W.W., Trust T. J. 1991; Form and functions of the regular surface array (S-layer) of Aeromonas salmonicida. Experientia 47:412–414
    [Google Scholar]
  14. Kobayashi Y., Ohta H., Kokeguchi S., Murayama Y., Kato K., Kurihara H., Fukui K. 1993; Antigenic properties of Campylobacter rectus (Wolinella recta) major S-layer proteins. FEMS Microbiol Lett 108:275–280
    [Google Scholar]
  15. Kokka R.P., Janda J. M. 1990; Isolation and identification of autoagglutinating serogroup 0:11 Aeromonas strains in the clinical laboratory. J Clin Microbiol 28:1297–1299
    [Google Scholar]
  16. Kokka R. P., Vedros N. A., Janda J. M. 1990; Electrophoretic analysis of the surface components of autoagglutinating surface array protein-positive and surface array protein-negative Aeromonas hydrophila and Aeromonas sobria. J Clin Microbiol 28:2240–2247
    [Google Scholar]
  17. Kokka R. P., Janda J. M., Oshiro L. S., Altwegg M., Shimada T., Sakazaki R., Brenner D. J. 1991a; Biochemical and genetic characterization of autoagglutinating phenotypes of Aeromonas species associated with invasive and noninvasive disease. J Infect Dis 163:890–894
    [Google Scholar]
  18. Kokka R. P., Vedros N. A., Janda J. M. 1991b; Characterization of classic and atypical serogroup O:11 Aeromonas: evidence that the surface array protein is not directly involved in mouse pathogenicity. Microb Fathog 10:71–79
    [Google Scholar]
  19. Kokka R. P., Vedros N. A., Janda J. M. 1992; Immunochemical analysis and possible biological role of an Aeromonas hydrophila surface array protein in septicaemia. J Gen Microbiol 138:1229–1236
    [Google Scholar]
  20. Kostrzynska M., Dooiey J. S. G., Shimojo T., Sakata T., Trust T.J. 1992; Antigenic diversity of the S-layer proteins from pathogenic strains of Aeromonas hydrophila and Aeromonas veronii biotype sobria. J Bacteriol 174:40–47
    [Google Scholar]
  21. Lachmann P.J. 1990; Complement genetics and host defense. Zentralbl Bakteriol 274:316–324
    [Google Scholar]
  22. Massad G., Arceneaux J. E. L., Byers B. R. 1991; Acquisition of iron from host sources by mesophilic Aeromonas species. J Gen Microbiol 137:237–241
    [Google Scholar]
  23. Messner P., Sleytr U. B. 1992; Crystalline bacterial cell-surface layers. Adv Microb Physiol 33:213–275
    [Google Scholar]
  24. Munn C. B., Ishiguro E. E., Kay W. W., Trust T. J. 1982; Role of surface components in serum resistance of virulent Aeromonas salmonicida. Infect Immun 36:1069–1075
    [Google Scholar]
  25. Paula S. J., Duffey P. S., Abbott S. L., Kokka R. P., Oshiro L. S., Janda J. M., Shimada T., Sakazaki R. 1988; Surface properties of autoagglutinating mesophilic aeromonads. Infect Immun 56:2658–2665
    [Google Scholar]
  26. Reed L.J., Muench H. 1938; A simple method of estimating fifty percent endpoints. Am J Hyg 27:493–497
    [Google Scholar]
  27. Tamplin M. L., Specter S., Rodrick G. E., Friedman H. 1983; Differential complement activation and susceptibility to human bactericidal action by Vibrio species. Infect Immun 42:1187–1190
    [Google Scholar]
  28. Taylor P.W. 1983; Bactericidal and bacteriolytic activity of serum against Gram-negative bacteria.. Microbiol Rev 47:46–83
    [Google Scholar]
  29. Viljanen P., Kayhty H., Vaara T. 1986; Susceptibility of Gram-negative bacteria to the syngergistic bactericidal action of serum and polymyxin B nonapeptide. Can J Microbiol 32:66–69
    [Google Scholar]
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