%0 Journal Article %A Wong, Cheryl M. %A Dilworth, Michael J. %A Glenn, Andrew R. %T Cloning and sequencing show that 4-hydroxybenzoate hydroxylase (PobA) is required for uptake of 4-hydroxybenzoate in Rhizobium leguminosarum %D 1994 %J Microbiology, %V 140 %N 10 %P 2775-2786 %@ 1465-2080 %R https://doi.org/10.1099/00221287-140-10-2775 %K aromatic substrate uptake %K sequence of pobA %K 4-hydroxybenzoate %K Rhizobium leguminosarum %I Microbiology Society, %X Mutants of Rhizobium leguminosarumbv. viciaeMNF300 and R. leguminosarumbv. trifoliiWU95 unable to accumulate 4-hydroxybenzoate lack 4-hydroxybenzoate hydroxylase. The capacity of these mutants to take up and grow on 4-hydroxybenzoate was restored by a 2.0 kb EcoRI-PstIDNA fragment. This contained only one ORF which had over 60% DNA sequence similarity with the structural gene for 4-hydroxybenzoate hydroxylase (pobA) from Pseudomonasspp. and Acinetobacter.Reported effects of metabolic inhibitors and substrate analogues on the apparent uptake of 4-hydroxybenzoate have now been shown to be due to their direct effect on 4-hydroxybenzoate hydroxylase. We propose that uptake of 4-hydroxybenzoate is via a metabolic ‘drag’ mechanism dependent on the activity of the pobAgene product. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-140-10-2775