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Three nitrogen-fixing bacteria, Azotobacter vinelandii, Beijerinckia indicaand Beijerinckia mobilis, were shown to contain large amounts of triterpenoids of the hopane series. In A. vinelandii, the major compound was a novel bacteriohopanepentol ether accompanied by a similar bacteriohopanetetrol derivative: in both compounds, the hopanoids are linked via an ether bond to a carbapseudopentose moiety often found in bacterial hopanoids. In the two Beijerinckiaspecies, diplopterol and 2β-methyldiplopterol were accumulated in much larger amounts than those usually recorded in hopanoid-producing eubacteria, while 2β-methyldiploptene was isolated for the first time from B. mobilis.Whereas in B. mobilisaminobacteriohopanetriol was the only C35hopanoid, the simultaneous presence of bacteriohopanetetrol and aminobacteriohopanetriol in B. indicais a rather unusual feature.
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