1887

Abstract

The white-rot basidiomycete produces two families of ligninolytic enzymes, namely manganese-dependent peroxidases (MnPs) and laccases, when growing in liquid cultures of defined composition. In medium containing 11 p.p.m. of Mn(II), up to seven isoenzymes of MnP and four isoenzymes of laccase were resolved by isoelectrofocusing (IEF), with pl values in the range 4.10-4.60 and 3.45-3.65, respectively. Occasionally, a fifth laccase isoform of pl 4.70 was also detected. In cultures with 25 and 40 p.p.m. of Mn(II). mainly the MnPs with higher pl values are produced. The isoenzyme pattern of MnP is not altered throughout the growth period of the fungus. MnP and laccase are also produced by when growing on wood chips of Highest levels of both enzymes were obtained during the first week of incubation. A second peak of MnP activity was observed during the fourth week, whereas very low levels of laccase were extracted from the chips after the second week of growth. IEF analysis showed that the pl values of these laccases are similar to those of laccases produced in liquid cultures, being in the range 3.45-3.65. In contrast, four isoforms of MnP were resolved during the first week of incubation on wood chips, with pl values of 4.40, 4.17, 4.04 and 3.53. This profile underwent a transition during the second week of growth, at the end of which isoforms of MnP with pl values of 3.53, 3.40, 3.30 and 3.20 were resolved by IEF. Immunoblotting studies showed that the molecular mass of MnP isoenzymes from liquid cultures was about 52.5 kDa, whereas the molecular masses of MnPs extracted from wood varied from 52.5 kDa to 62.5 kDa upon ageing of the cultures. The amino terminal sequences of seven MnP isoenzymes were determined. The consensus sequences of MnPs from liquid and solid cultures were clearly distinct, although both showed homology to MnPs from related white-rot fungi.

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1994-10-01
2022-01-25
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References

  1. Akhtar M., Attridge M.C., Myers G.C., Kirk T.K., Blanchette R.A. Biomechanical pulping of loblolly pine with different strains of the white-rot fungus Ceriporiopsis subvermispora. Tappi J 1992; 75:105–109
    [Google Scholar]
  2. Akhtar M., Attridge M.C., Myers G.C., Blanchette R.A. Biomechanical pulping of loblolly pine chips with selected white-rot fungi. Holdorscbtlng 1993; 47:36–40
    [Google Scholar]
  3. Archibald F.S. A new assay for lignin-type peroxidases employing the dye Azure B. Appl Environ Microbiol 1992; 58:3110–3116
    [Google Scholar]
  4. Blanchette R.A., Burnes T.A., Eerdmands M.M., Akhtar M. Evaluating isolates of Phanerochaete chrjsosporium and Ceriporiopsis subvermispora for use in biological pulping processes. Holdorscbtlng 1992; 46:109–115
    [Google Scholar]
  5. Bonnarme P., Jeffries T.W. Mn(II) regulation of lignin peroxidases and manganese-dependent peroxidases from lignin- degrading white rot fungi. Appl Environ Microbiol 1990; 56:210–217
    [Google Scholar]
  6. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248–254
    [Google Scholar]
  7. Coll P.M., Tabernero C., Santa-Marfa R., Perez P. Characterization and structural analysis of the laccase I gene from the newly isolated ligninolytic basidiomycete PM1 (CECT 2971). Appl Environ Microbiol 1993; 59:4129–4135
    [Google Scholar]
  8. Cullen D., Kersten P. Fungal enzymes for lignocellulose degradation. In Applied Molecular Genetics of Filamentous Fungi 1992 Edited by Kinghorn J.R., Turner G. London: Chapman and Hall; pp 100–131
    [Google Scholar]
  9. Das S.B., Reddy C.A. Characterization of extracellular peroxidases produced by acetate-buffered cultures of the lignin- degrading basidiomycete Phanerochaete chrjsosporium. FEMS Microbiol Lett 1990; 69:221–224
    [Google Scholar]
  10. Datta A., Bettermann A., Kirk T.K. Identification of a specific manganese peroxidase among ligninolytic enzymes secreted by Phanerochaete chrjsosporium during wood decay. Appl Environ Microbiol 1991; 57:1453–1460
    [Google Scholar]
  11. Forrester I.T., Grabski A.C., Mishra C., Kelley B.L., Strickland W.N., Leatham G.F., Burgess R.R. Characteristics and N-terminal aminoacid sequence of a manganese peroxidase purified from Eentinula edodes cultures grown on commercial wood substrate. Appl Microbiol Biotecbnol 1990; 33:359–365
    [Google Scholar]
  12. Galliano H., Gas G., Seris J.L., Boudet A.M. Lignin degradation by Rigidoporus lignosus involves synergistic action oftwo oxidizing enzymes: Mn peroxidase and laccase. Enz Microb Technol 1991; 13:478–482
    [Google Scholar]
  13. Geiger J.P., Rio B., Nandris D., Nicole M. Laccases of Rigidoporus lignosus and Phellinus noxius. Appl Biochem Biotechnol 1986; 12:121–133
    [Google Scholar]
  14. limura Y., Takenouchi K., Nakamura M., Kawai S., Katayama Y., Morohoshi M. Cloning and sequence analysis of laccase genes and its use for a expression vector in Coriolus versicolor. In Biotechnology in the Pulp and Paper Industry 1992 Edited by Kuwahara M., Shimada M. Tokyo: UNI Publishers; pp 427–431
    [Google Scholar]
  15. Johansson T., Nyman P.O. Isozymes of the lignin peroxidase and manganese (II) peroxidase from the white-rot basidiomycete Trametes versicolor. I. Isolation of enzyme forms and characterization of physical and catalytic properties. Arch Biochem Biophys 1993; 300:49–56
    [Google Scholar]
  16. Johansson T., Welinder K.G., Nyman P.O. Isozymes of the lignin peroxidase and manganese (II) peroxidase from the white-rot basidiomycete Trametes versicolor. II. Partial sequences peptide maps and aminoacid and carbohydrate compositions.. Arch Biochem Biophys 1993; 300:57–62
    [Google Scholar]
  17. Karhunen E., Kantelinen A., Niku-Paavola M.-L. Mn-dependent peroxidase from the lignin-degrading white-rot fungus Phlebia radiata. Arch Biochem Biophys 1990; 279:25–31
    [Google Scholar]
  18. Kojima Y., Tsukuda Y., Kawai Y., Tsukamoto A., Sugiura J., Sakaino M., Kita Y. Cloning, sequence analysis and expression of ligninolytic phenol oxidase genes of the white-rot basidiomycete Coriolus hirsutus. J Biol Chem 1990; 265:15224–15230
    [Google Scholar]
  19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  20. Leatham G.F. A chemically defined medium for the fruiting of Lentinus edodes. Mycologia 1983; 75:5905–908
    [Google Scholar]
  21. Leisoia M.S.A., Kozulic B., Meussdoerffer F., Fiechter A. Homology among multiple extracellular peroxidases from Phanerochaete chrysosporium. J Biol Chem 1987; 262:419–424
    [Google Scholar]
  22. Maltseva O.V., Niku-Paavola M.-L., Leontievsky A.A., Myasoedova N.M., Golovleva L.A. Ligninolytic enzymes of the white-rot fungus Panus tigrinus. Biotechnol Appl Biochem 1991; 13:291–302
    [Google Scholar]
  23. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 1987; 262:10035–10038
    [Google Scholar]
  24. Morohoshi N. Laccases from the ligninolytic fungus Coriolus versicolor. In Enzymes in Biomass Conversion 1991 Edited by Leatham G., Himmel M.E. Washington, DC: American Chemical Society; ACS Symposium Series 460, pp 207–204
    [Google Scholar]
  25. Niku-Paavola M.-L., Karhunen E., Salola P., Raunio V. Ligninolytic enzymes of the white-rot fungus Phlebia radiata. Biochem J 1988; 254:877–884
    [Google Scholar]
  26. Orth A., Royse D.J., Tien M. Ubiquity of lignin- degrading peroxidases among various wood-degrading fungi. Appl Environ Microbiol 1993; 59:4017–4023
    [Google Scholar]
  27. Otjen L., Blanchette R., Effland M., Leatham G. Assessment of 30 white-rot basidiomycetes for selective lignin degradation. Holzforschung 1987; 41:343–349
    [Google Scholar]
  28. Pease E.A., Tien M. Heterogeneity and regulation of manganese peroxidases from Phanerochaete chrysosporium. J Bacterial 1992; 174:3532–3540
    [Google Scholar]
  29. Pease E.A., Andrawis A., Tien M. Manganese- dependent peroxidases from Phanerochaete chrysosporium-primary structure deduced from cDNA sequence. J Biol Chem 1989; 264:13531–13535
    [Google Scholar]
  30. Perie F.H., Gold M.H. Manganese regulation of manganese peroxidase expression and lignin degradation by the white-rot fungus Dichomitus squalens. Appl Environ Microbiol 1991; 57:2240–2245
    [Google Scholar]
  31. Pribnow D., Mayfield M.B., Nipper V.J., Brown J.A., Gold M.H. Characterization of cDNA encoding a manganese peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium. J Biol Chem 1989; 264:5036–5040
    [Google Scholar]
  32. Ruttimann C., Schwember E., Salas L., Cullen D., Vicuna R. Ligninolytic enzymes of the white-rot basidiomycetes Phlebia brevispora and Ceriporiopsis subvermispora. Biotechnol Appl Biochem 1992a; 16:64–76
    [Google Scholar]
  33. Ruttimann C., Salas L., Vicuna R. Studies on the ligninolytic system of the white-rot fungus Ceriporiopsis subver mispora. In Biotechnology in the Pulp and Paper Industry 1992b Edited by Kuwahara M., Shimada M. Tokyo: UNI Publishers; pp 243–248
    [Google Scholar]
  34. Ruttimann-Johnson C., Salas L., Vicuna R., Kirk T.K. Extracellular enzyme production and synthetic lignin minerali zation by Ceriporiopsis subvermispora. Appl Environ Microbiol 1993; 59:1792–1797
    [Google Scholar]
  35. Ruttimann-Johnson C., Cullen D., Lamar R. Manganese peroxidases of the white-rot fungus Phanerochaete sordida. Appl Environ Microbiol 1994; 60:599–605
    [Google Scholar]
  36. Saloheimo M., Niku-Paavola M.-L., Knowles J.K.C. Isolation and structural analysis of the laccase gene from the lignin- degrading fungus Phlebia radiata. J Gen Microbiol 1991; 137:1537–1544
    [Google Scholar]
  37. Tien M., Kirk T.K. Lignin-degrading enzyme from Phanerochaete chrysosporium-purification characterization and cata lytic properties of a unique H2Oa requiring oxygenase. Proc Natl Acad Sci USA 1984; 81:2280–2284
    [Google Scholar]
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