RT Journal Article SR Electronic(1) A1 Herrero, Mónica A1 de los Reyes-Gavilán, Clara G A1 Caso, José L. A1 Suárez, Juan E.YR 1994 T1 Characterization of ø393-A2, a bacteriophage that infects Lactobacillus casei JF Microbiology, VO 140 IS 10 SP 2585 OP 2590 DO https://doi.org/10.1099/00221287-140-10-2585 PB Microbiology Society, SN 1465-2080, AB Bacteriophage ø393-A2, isolated from an artisanal cheese whey sample, is a temperate phage able to generate stable lysogens through integration of its DNA into the bacterial genome. One-step growth kinetics of its lytic development revealed eclipse and latent periods of 100 and 140 min, respectively, with a burst size of about 200 p.f.u. per infected cell. ϕ393-A2 virions have an isometric head and a long, non-contractile tail terminating in a baseplate. The capsid is composed of two major and at least nine minor structural polypeptides. The phage genome consists of a double-stranded DNA molecule of 44 kbp bearing 3′-protruding cohesive ends. A physical map of the phage DNA has been constructed for six restriction enzymes. The whole ϕ393-A2 genome has been cloned in Escherichiacoli using plasmid- and phage-derived cloning vectors., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-140-10-2585