@article{mbs:/content/journal/micro/10.1099/00221287-140-10-2585, author = "Herrero, Mónica and de los Reyes-Gavilán, Clara G and Caso, José L. and Suárez, Juan E.", title = "Characterization of ø393-A2, a bacteriophage that infects Lactobacillus casei", journal= "Microbiology", year = "1994", volume = "140", number = "10", pages = "2585-2590", doi = "https://doi.org/10.1099/00221287-140-10-2585", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-140-10-2585", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "bacteriophage", keywords = "cloning", keywords = "lysogenization", keywords = "Lactobacillus casei", keywords = "development", abstract = "Bacteriophage ø393-A2, isolated from an artisanal cheese whey sample, is a temperate phage able to generate stable lysogens through integration of its DNA into the bacterial genome. One-step growth kinetics of its lytic development revealed eclipse and latent periods of 100 and 140 min, respectively, with a burst size of about 200 p.f.u. per infected cell. ϕ393-A2 virions have an isometric head and a long, non-contractile tail terminating in a baseplate. The capsid is composed of two major and at least nine minor structural polypeptides. The phage genome consists of a double-stranded DNA molecule of 44 kbp bearing 3′-protruding cohesive ends. A physical map of the phage DNA has been constructed for six restriction enzymes. The whole ϕ393-A2 genome has been cloned in Escherichiacoli using plasmid- and phage-derived cloning vectors.", }