1887

Abstract

Bacteriophage ø393-A2, isolated from an artisanal cheese whey sample, is a temperate phage able to generate stable lysogens through integration of its DNA into the bacterial genome. One-step growth kinetics of its lytic development revealed eclipse and latent periods of 100 and 140 min, respectively, with a burst size of about 200 p.f.u. per infected cell. ϕ393-A2 virions have an isometric head and a long, non-contractile tail terminating in a baseplate. The capsid is composed of two major and at least nine minor structural polypeptides. The phage genome consists of a double-stranded DNA molecule of 44 kbp bearing 3′-protruding cohesive ends. A physical map of the phage DNA has been constructed for six restriction enzymes. The whole ϕ393-A2 genome has been cloned in coli using plasmid- and phage-derived cloning vectors.

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1994-10-01
2024-03-29
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References

  1. Becker A., Murialdo H. Bacteriophage lambda DNA: the beginning of the end. J Bacterial 1990; 6:2819–2824
    [Google Scholar]
  2. Bimboim H.C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7:1513–1523
    [Google Scholar]
  3. Bolívar F., Rodríguez R.L., Greene P.J., Betlach M.C., Heynecker H.C., Boyer H.N. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 1977; 2:95–113
    [Google Scholar]
  4. Bradley D.E. Ultrastructure of bacteriophages and bacteriocins. Bacteriol Rev 1967; 31:230–314
    [Google Scholar]
  5. Bullock W.O., Fernández J.M., Short J.M. XL-1 Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques 1987; 5:376–378
    [Google Scholar]
  6. Chang A.C., Cohen S.N. Construction and character-ization of amplificable multicopy DNA cloning vehicles derived from the pl5 cryptic miniplasmid. J Bacteriol 1978; 134:1141–1156
    [Google Scholar]
  7. Dupont L., Boizet B., Mata M., Ritzenthaler P. Comparison of a late/early region in temperate and virulent phages of Eactobacillus delbrueckii 1994 EMBO-FEMS Meeting on Bacterial Viruses, Gargnano Italy
    [Google Scholar]
  8. Ellis D.M., Dean D.H. Nucleotide sequence of the cohesive single-stranded ends of Bacillus subtilis temperate bac-teriophage 105. J Virol 1985; 55:513–515
    [Google Scholar]
  9. Fitzmaurice W.P., Waldman A.S., Benjamin R.C., Huang P.C., Scocca J.J. Nucleotide sequence and properties of the cohesive DNA termini from bacteriophage HPlcl of Elaemophilus influenzae Rd. Gene 1984; 31:197–203
    [Google Scholar]
  10. González De Llano D., Ramos M., Rodriguez A., Montilla A., Juárez M. Microbiological and physicochemical character-istics of Gamonedo Blue Cheese during ripening. Int Dairy J 1992; 2:121–135
    [Google Scholar]
  11. Hayashi T., Matsumoto H., Ohnishi M., Terawaki Y. Molecular analysis of a cytotoxin-converting phage, CTX of Pseudomonas aeruginosa', structure of the attP-cos-ctx region and integration into the serine tRNA gene. Mol Microbiol 1993; 7:657–667
    [Google Scholar]
  12. Holmes D.S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 1981; 114:193–197
    [Google Scholar]
  13. Jarvis A.W. Bacteriophages of lactic acid bacteria. J Dairy Sci 1989; 72:3406–3428
    [Google Scholar]
  14. Jarvis A.W., Fitzgerald G.F., Mata M., Mercenier A., Neve H., Powell I.P., Ronda C., Saxelin M., Teuber M. Species and type phages of lactococcal bacteriophages. Intervirology 1991; 32:2–9
    [Google Scholar]
  15. Keogh B.P. Adsorption, latent period and burst size of phages of some strains of lactic streptococci. J Dairy Res 1973; 40:303–309
    [Google Scholar]
  16. Khosaka T. Physicochemical properties of virulent Lacto¬bacillus phage containing DNA with cohesive ends. J Gen Virol 1977; 37:209–214
    [Google Scholar]
  17. De Klerk H.C., Coetzee J.N. The characterization of a series of Lactobacillus bacteriophages. J Gen Microbiol 1963; 32:61–67
    [Google Scholar]
  18. Kobler L., Schwertfirm G., Schmieger H., Bolotin A., Sladkova I. Construction and transduction of a shuttle vector bearing the cos site of Streptomyces phage C31 and determination of its cohesive ends. FEMS Microbiol Lett 1991; 78:347–354
    [Google Scholar]
  19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  20. Lillehaug D., Lindqvist B.H., Birkeland N.K. Character¬ization of LC3, a Lactococcus lactis subsp. cremoris temperate bacteriophage with cohesive single-stranded DNA ends. Appi Environ Microbiol 1991; 57:3206–3211
    [Google Scholar]
  21. Matthews R.E.F. The classification and nomenclature of viruses. Intervirology 1981; 16:53–60
    [Google Scholar]
  22. Mercenier A., Lemoine Y. Genetics of Streptococcus thermophilus: a review. J Dairy Sci 1989; 72:3444–3454
    [Google Scholar]
  23. Morrisey J.M. Silver stain for proteins in polyacrylamide gels : a modified procedure with enhanced uniform sensitivity. Anal Biochem 1981; 117:307–310
    [Google Scholar]
  24. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Laboratory Manual, 2nd edn 1989 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Scheirlink T., Mahillon J., Joos H., Dhaese P., Michiels F. Integration and expression of a-amylase and endoglucanase genes in the Lactobacillus plantarum chromosome. Appi Environ Microbiol 1989; 55:2130–2137
    [Google Scholar]
  26. Séchaud L., Cluzel P.-J., Rousseau M., Baumgartner A., Accolas J.-P. Bacteriophages of lactobacilli. Biochimie 1988; 70:401–410
    [Google Scholar]
  27. Sechaud L., Rousseau M., Fayard B., Callegari M.L., Quenee P., Accolas J.-P. Comparative study of 35 bacteriophages of Lactobacillus helveticus\ morphology and host range. Appl Environ Microbiol 1992; 58:1011–1018
    [Google Scholar]
  28. Shimizu-Kadota M., Tsuchida N. Physical mapping of the virion and the prophage DNAs of a temperate Lactobacillus phage FSW. J Gen Microbiol 1984; 130:423–430
    [Google Scholar]
  29. Shimizu-Kadota M., Sakurai T., Tsuchida N. Prophage origin of a virulent phage appearing on fermentations of Lacto¬bacillus casei S-l. Appl Environ Microbiol 1983; 45:669–674
    [Google Scholar]
  30. Short J.M., Fernandez J.M., Sorge J.A., Huse W.D. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res 1988; 16:7583–7590
    [Google Scholar]
  31. Sing W.D., Klaenhammer T.R. A strategy for rotation of different bacteriophage defenses in a lactococcal single strain starter culture system. Appl Environ Microbiol 1993; 59:365–372
    [Google Scholar]
  32. Sozzi T., Poulin J.M., Maret R. Effect of incubation temperature on the development of lactic acid bacteria and their phages. J Dairy Res 1978; 45:259–265
    [Google Scholar]
  33. Sozzi T., Watanabe K., Stetter K., Smiley M. Bacteriophages of the genus Lactobacillus. Intervirology 1981; 16:129–135
    [Google Scholar]
  34. Stetter K.O., Priess H., Delius H. Lactobacillus casei phage PL-1. Molecular properties and first transcription studies in vivo and in vitro. Virology 1978; 87:1–12
    [Google Scholar]
  35. Suarez J.E., Chater K.F. Development of a DNA cloning system in Streptomyces using phage vectors. Cienc Biol 1981; 6:99–110
    [Google Scholar]
  36. Watanabe K., Takesue S., Ishibashi K. DNA of phage PL-1 active against Lactobacillus casei ATCC 27092. Agric Biol Chem 1980; 44:453–455
    [Google Scholar]
  37. Watanabe K., Takesue S., Ishibashi K., Iwamoto T., Kondo T.I. Fine structure of PL-1 phage active against Lactobacillus casei. J Gen Microbiol 1982; 28:1–6
    [Google Scholar]
  38. Yanisch-Perron C., Vieira J., Messing J. Improved Ml 3 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 1985; 33:103–119
    [Google Scholar]
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