SUMMARY: Several criteria of normal rumen function which can be applied to studies with the whole rumen microbial population are suggested. These include: the maintenance of numbers and normal appearance of the bacteria, selenomonads and protozoa of the rumen; the maintenance of normal rates of digestion of cellulose, starch and protein, and of normal interactions between these; the ability to predict quantitative results . An ‘artificial rumen’ was constructed, consisting of a cellophan sac containing rumen liquor and substrate dialysing against a complex mineral solution whose composition was based on that found in rumen liquor, the whole being incubated at 39° in an atmosphere of nitrogen and carbon dioxide. This system was shown to meet the criteria which are suggested, with reasonable success for periods of about 8 hr.; over longer periods an increasing failure to meet the biological criteria was seen. For the microbial population to remain normal in numbers and activity it was shown to be necessary to use as test substrate only substances similar to the diet fed to the animal from which the rumen liquor inoculum was taken.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error