1887

Abstract

SUMMARY: Five A strains and three B strains of influenza virus were purified and found to contain ribonucleic acid in amounts varying from 0·75 to 1·1 %. The proportion of the purine and pyrimidine derivatives in the nucleic acid of each strain was determined. When the ratio adenine + uracil : guanine + cytosine was evaluated, the following values were obtained: A strains —PR 8, 1·27 ± 0·02; MEL, 1·22 ± 0·01; WSE, 1·26 0±01; SWINE, 1·24 ± 0·04; CAM, 1·28 ± 0·01. B strains—LEE, 1·42 ± 0·04; MIL, 1·43 ± 0·05; ROB, 1·38 ± 0·01. The nucleic acid content of PR8 virus preparations of varying degrees of incompleteness was determined both by specific absorption at 260 m. and by estimation of phosphorus present in the nucleic acid extract. As measured by both methods, virus preparations of low infectivity were found to have a decreased nucleic acid content. The demonstration of specific differences in the nucleic acid of A and B strains and of the relationship between the infectivity and nucleic acid content of the virus affords strong evidence that the nucleic acid is an intrinsic part of the influenza virus particle.

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/content/journal/micro/10.1099/00221287-14-3-623
1956-07-01
2022-01-29
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References

  1. Ada G.L., French E.L. 1950; Purification of the receptor destroying enzyme of V. cholerae. Aust. J. Sci. 13:82
    [Google Scholar]
  2. Ada G.L., Gottschalk A. 1956; The component sugars of the influenza virus particle.. Biochem.J. in the Press
    [Google Scholar]
  3. Ada G.L., Perry B.T. 1954; The nucleic acid content of influenza virus.. Aust. J. exp. Biol. med. Sci. 32:453
    [Google Scholar]
  4. Ada G.L., Perry B.T. 1955a; Infectivity and nucleic acid content of influenza virus.. Nature; Lond.: 175209
    [Google Scholar]
  5. Ada G.L., Perry B.T. 1955b; Specific differences in the nucleic acids from A and B strains of influenza virus.. Nature; Lond.: 175854
    [Google Scholar]
  6. Burnet F.M. 1935; Influenza virus isolated from an Australian epidemic.. Med. J. Aust. ii:651
    [Google Scholar]
  7. Burnet F.M. 1955 Principles of Animal Virology pp. 135–138182–184 New York: Acad. Press. Inc.;
    [Google Scholar]
  8. Burnet F.M., Anderson S.G. 1947; Sporadic and minor epidemic incidence of influenza A in Victoria, 1945–46. I. Phase behaviour of influenza A strains in relation to epidemic characteristics.. Aust. J. exp. Biol. med. Sci. 25:235
    [Google Scholar]
  9. Burnet F.M., Lind P.E., Stevens K.M. 1955; Production of incomplete influenza virus in the de-embryonated egg. Aust. J. exp. Biol. med. Sci. 33:127
    [Google Scholar]
  10. Burnet F.M., Stone J.D., Anderson S.G. 1946; An epidemic of influenza B in Australia.. Lancet i:807
    [Google Scholar]
  11. Chargaff E., Zamenhof S. 1948; The isolation of highly polymerised desoxy-pentosenucleic acid from yeast cells.. J. biol. Chem. 173:327
    [Google Scholar]
  12. Epstein H.T. 1953; The properties of bacteriophages.. Advanc. Virus Res. 1:1
    [Google Scholar]
  13. Fazekas De St Groth S., Graham D.M. 1954; The production of incomplete virus particles among influenza strains: experiments in eggs.. Brit. J. exp. Path. 35:60
    [Google Scholar]
  14. Finter N.B., Liu O.C., Henle W. 1955; Studies on host-virus interactions in the chick embryo-influenza virus system. 10. An experimental analysis of the von Magnus phenomenon.. J. exp. Med. 101:461
    [Google Scholar]
  15. Francis T. 1934; Transmission of influenza by filterable virus.. Science 80:457
    [Google Scholar]
  16. Francis T. 1940; New type of virus from epidemic influenza.. Science 92:405
    [Google Scholar]
  17. Hanes C.S., Hird F.J.R., Isherwood F.A. 1952; Enzymic transpeptidation reactions involving γ-glutamvl peptides and α amino-acyl peptides.. Biochem. J. 51:25
    [Google Scholar]
  18. Horsfall F.L. 1954; On the reproduction of influenza virus. Quantitative studies with procedures which enumerate infective and haemagglutinating virus particles.. J. exp. Med. 100:135
    [Google Scholar]
  19. Jeener R., Lemoine P. 1953; Occurrence in plants infected with tobacco- mosaic virus of a crystallizable antigen devoid of ribonucleic acid.. Nature; Lond.: 171935
    [Google Scholar]
  20. Kay E.R.M., Dounce A.L. 1953; The preparation of sodium ribonucleate with the use of sodium dodecyl sulphate.. J. Amer. chem. Soc. 75:4041
    [Google Scholar]
  21. Knight C.A. 1947; The nucleic acid and carbohydrate of influenza virus.. J. exp. Med. 85:99
    [Google Scholar]
  22. Ledinko N., Perry B. 1955; Studies with influenza virus B of recent human origin. 1. Adaptation to the mouse lung.. J. Immunol. 74:371
    [Google Scholar]
  23. Liu O.C., Blank H., Spizizen J., Henle W. 1954; The incorporation of radioactive phosphorus into influenza virus.. J. Immunol. 73:415
    [Google Scholar]
  24. Magnus P.Von. 1946; Studies on interference in experimental influenza. I. Biological observations.. Arkiv. Kemi., Swed. Acad. Sci. 24 B:7
    [Google Scholar]
  25. Magnus P.Von. 1954; Incomplete forms of influenza virus.. Advanc. Virus Res. 2:59
    [Google Scholar]
  26. Markham R. 1951; Physico-chemical studies of the turnip yellow mosaic virus.. Disc. Faraday Soc. 11:221
    [Google Scholar]
  27. Markham R., Smith J.D. 1951; Chromatographic studies of nucleic acids. 4. The nucleic acid of the turnip yellow mosaic virus, including a note on the nucleic acid of the tomato bushy stunt virus.. Biochem. J. 49:401
    [Google Scholar]
  28. Reed L.J., Muench H. 1938; Simple method of estimating 50 per cent end points.. Amer. J. Hyg. 27:493
    [Google Scholar]
  29. Rich A., Dunitz J.D., Newmark P. 1955; Structure of polymerized tobacco plant protein and tobacco mosaic virus.. Nature; Lond.: 1751074
    [Google Scholar]
  30. Schmidt G., Thannhauser S.J. 1945; A method for the detection of desoxyribonucleic acid, ribonucleic acid, and phospho-proteins in animal tissues.. J. biol. Chem. 161:83
    [Google Scholar]
  31. Shack J., Jenkins R.J., Thompsett J.M. 1953; The interaction of ions and desoxypentose nucleic acid of calf thymus.. J. biol. Chem. 203:373
    [Google Scholar]
  32. Shope R.E. 1931; Swine influenza; experimental transmission and pathology.. J. exp. Med. 54:349
    [Google Scholar]
  33. Smith J.D., Markham R. 1950; Chromatographic studies on nucleic acids. 2. The quantitative analysis of ribonucleic acids.. Biochem J. 46:509
    [Google Scholar]
  34. Smith W., Andrewes C.H., Laidlaw P.P. 1933; Virus obtained from influenza patients.. Lancet ii:66
    [Google Scholar]
  35. Wyatt G.R. 1951; The purine and pyrimidine composition of deoxypentose nucleic acids.. Biochem. J. 48:584
    [Google Scholar]
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