SUMMARY: The urease activity of has been studied in washed suspensions and in cell-free extracts of the micro-organism. The urease is constitutive, and whole organisms have a urease activity of 17,000-22,000μg. NH/mg. dry wt. organisms/hr. in 0·5-urea in phosphate buffer at pH 7·0. In a cell-free extract the enzyme is optimally active at pH 7·5, has a Michaelis constant of 0·030, and a temperature velocity constant of 7800 cal.; the activity is inhibited by atmospheric oxygen and by thiourea, but not by sixteen other analogues of urea tested.


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