1887

Abstract

Summary: Apple pectin in a complex organic medium partly protected strain B from lysis by T phage. It was not bactericidal or virucidal. The rate of adsorption of the phage was unaltered, but part of the initially adsorbed phage could be eluted with distilled water at 0°, as the second irreversible step of adsorption was inhibited by pectin. It was shown in one-step growth and single cell burst experiments that phage multiplication was reduced. The release of any formed phage from the host was not affected. The protective effect of the pectin resulted from the failure of some of the phage particles to penetrate into the host cell and from its action in decreasing phage synthesis in those cells where penetration did take place. It is suggested that this non-specific polysaccharide may exert its protective action because of its polymeric electrolyte nature.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-14-2-323
1956-04-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/14/2/mic-14-2-323.html?itemId=/content/journal/micro/10.1099/00221287-14-2-323&mimeType=html&fmt=ahah

References

  1. Adams M.H. 1950; Methods of study of bacterial viruses. Meth. med. Res. 2:3
    [Google Scholar]
  2. Ashenburg N. J., Sandholzer L. A., Sherp H. W., Berry G. P. 1940; The influence of bacterial and non-bacterial polysaccharides upon bacteriophage. J. Bact. 39:71
    [Google Scholar]
  3. Ashenburg N.J., Sandholzer L.A., Sherp H.W., Berry G.P. 1950; The inhibition of bacteriophage and non-bacterial polysaccharides. J. Bact. 59:681
    [Google Scholar]
  4. Bichovsky-Slomnitzky L. 1953; Data cited by Katchalsky, A. Polyelectrolytes. Endeavour 12:90
    [Google Scholar]
  5. Burnet F.M. 1934; The bacteriophage inactivating agent of bacterial extracts. J. Path. Bact. 38:285
    [Google Scholar]
  6. Garen A., Puck T.T. 1951; The first two steps of the invasion of host cells by bacterial viruses. II. J. exp. Med. 94:177
    [Google Scholar]
  7. Gough G.A.C., Burnet F.M. 1934; The chemical nature of the bacteriophage inactivating agent in bacterial extracts. J. Path. Bact. 38:301
    [Google Scholar]
  8. D’Herelle F. 1926 The Bacteriophage and its Behaviour p. 63 English translation London:: Baillière, Tindall and Cox.;
    [Google Scholar]
  9. Kay D. 1952; The intracellular multiplication of coli bacteriophage T5st. Brit. J. exp. Path. 33:236
    [Google Scholar]
  10. Kleczkowski J., Kleczkowski A. 1952; Effect of specific polysaccharides from the host bacteria and of ribonuclease on the multiplication of rhizobium phages. J. gen. Microbiol. 7:340
    [Google Scholar]
  11. Levine P., Frisch A.W. 1933; Specific inhibition of phage by bacterial extracts. Proc. Soc. exp. Biol. N.Y.: 30:993
    [Google Scholar]
  12. Maurer F.D., Woolley D.W. 1948; Protection of Escherichia coli against bacteriophage with citrus pectin. Proc. Soc. exp. Biol. N.Y.: 67:379
    [Google Scholar]
/content/journal/micro/10.1099/00221287-14-2-323
Loading
/content/journal/micro/10.1099/00221287-14-2-323
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error