SUMMARY: A method for isolating and identifying high acid-yielding mutants of is described. This involves cultivation of the organisms on absorbent paper soaked in an indicator medium. The advantages of the technique and the criteria used for selecting biochemically interesting mutants are briefly described. The greater acid production of mutants selected by paper culture has been confirmed by comparing yields of citric acid in surface and submerged fermentations with those given by the wild type strain.


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  1. Fisher R. A. 1950 Statistical Methods for Research Workers, 11th ed.. revised London: Oliver and Boyd;
    [Google Scholar]
  2. Foster J. W., Davis H. 1949; Detection and occurrence of acid-producing fungi. Bull. Torrey bot. Cl. 76:174
    [Google Scholar]
  3. Gardner J. F., James L. V., Rubbo S. D. 1956; Production of citric acid by mutants of Aspergillus niger. J. gen. Microbiol. 14:228
    [Google Scholar]
  4. Quilico A., Panizzi L., Visconti N. 1949; Richerche sulla fermentazione citrica con mutanti di Aspergillus niger. R.C. Accad. Lincei 6:40
    [Google Scholar]
  5. Shu P., Johnson M. J. 1947; Effect of the composition of the sporulation medium on citric acid production of Aspergillus niger in submerged culture. J. Bact. 54:161
    [Google Scholar]

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