SUMMARY: Tetrazolium (2:3:5-triphenyltetrazolium chloride) was used in a glucose nutrient medium to show the considerable differences in reducing properties between some of the Lancefield group D streptococci. The ability to reduce tetrazolium to formazan, in a glucose-containing medium, at an initial pH 6·0, distinguishes strains of from those of (Orla-Jensen) which for many years have been classified in the same species as . Differences in reducing properties correspond with many other tests now used to separate these species. Measurements of the oxidation-reduction potentials in growing cultures showed that produced an value about 150 mV. lower than . Confirmation of the differences in reducing properties of these species was obtained by testing 68 freshly isolated strains of and 34 strains of .


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