1887

Abstract

SUMMARY: No -galactosidase was formed when nitrogen-depleted cells of 1433 were treated with lactose unless a source of nitrogen was added to the cells. In the presence of an exogenous nitrogen source there was an appreciable delay between the addition of lactose and the appearance of induced -galactosidase activity. In contrast, induced increases of nitrate- and tetrathionate-reductase activities were developed without appreciable delay when depleted cells were treated with nitrate and tetrathionate, respectively, in the absence of additional nitrogen sources. The total amounts of the reductase activities developed in depleted cells were less than those obtained with non-depleted cells. The total amount of nitrate reductase activity formed was only slightly increased in the presence of ammonium sulphate, whereas normal degrees of activity were attained in the presence of casein hydrolysate. Ammonium sulphate markedly stimulated the formation of tetrathionate reductase activity.

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/content/journal/micro/10.1099/00221287-14-1-47
1956-02-01
2021-10-27
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References

  1. Cohn M., Torriani A-M. 1953; The relationships in biosynthesis of the β-galacto-sidase and Pz-proteins in Escherichia coli. Biochim. biophys. Acta 10:280
    [Google Scholar]
  2. Gale E.F., Folkes J.P. 1953; The assimilation of amino-acids by bacteria. 15. Actions of antibiotics on nucleic acid and protein synthesis in Staphylococcus aureus. . Biochem. J. 53:493
    [Google Scholar]
  3. Gale E.F., Folkes J.P. 1954; Effect of nucleic acids on protein synthesis and amino-acid incorporation in disrupted staphylococcal cells. Nature; Lond.: 1731223
    [Google Scholar]
  4. Halvorson H.O., Spiegelman S. 1952; The inhibition of enzyme formation by amino acid analogues. J. Bact. 64:207
    [Google Scholar]
  5. Halvorson H.O., Spiegelman S. 1953a; The effect of free amino acid pool levels on the induced synthesis of enzymes. J. Bact. 65:496
    [Google Scholar]
  6. Halvorson H.O., Spiegelman S. 1953b; Net utilization of free amino acids during the induced synthesis of maltozymase in yeast. J. Bact. 65:601
    [Google Scholar]
  7. Hogness D.S., Cohn M., Monod J. 1955; Studies on the induced synthesis of β-galactosidase in Escherichia coli: The kinetics and mechanism of sulphur incorporation. Biochim. biophys. Acta 16:99
    [Google Scholar]
  8. Lederberg J. 1950; .The beta-d-galactosidase of Escherichia coli, strain K-12. J. Bact. 60:381
    [Google Scholar]
  9. Monod J., Cohen-Bazire G., Cohn M. 1951; Sur la biosynthèse de la β-galacto-sidase (lactase) chez Esch. coli. La spécificité de l’induction. Biochim. biophys. Acta 7:585
    [Google Scholar]
  10. Monod J., Cohn M. 1953; Sur le mécanisme de la synthèse d’une protéine bacteérienne. La β-galactosidase d’E. coli. In Microbial Metabolism, Symp. Vlth int. Congr. Microbiol. p. 42
    [Google Scholar]
  11. Nicholas D.J.D., Nason A. 1954; Molybdenum and nitrate reductase. II. Molybdenum as a constituent of nitrate reductase. . J. biol. Chem. 207:353
    [Google Scholar]
  12. Nicholas D.J.D., Nason A. 1955; Diphosphopyridine nucleotide-nitrate reductase from Escherichia coli. . J. Bact. 69:580
    [Google Scholar]
  13. Pollock M.R. 1946; Adaptation of ‘nitratase’ in washed suspensions of bacteria. . Brit. J. exp. Path. 27:419
    [Google Scholar]
  14. Rotman B., Spiegelman S. 1954; On the origin of the carbon in the induced synthesis β-galactosidase in Escherichia coli. . J. Bact. 68:419
    [Google Scholar]
  15. Spiegelman S., Dunn R. 1947; Interactions between enzyme-forming systems during adaptation. J. gen. Physiol. 31:153
    [Google Scholar]
  16. Spiegelman S., Halvorson H.O. 1953; The nature of the precursor in the induced synthesis of enzymes. Adaptation in Micro-organisms. Third Symp. Soc. gen. Microbiol p. 98 Cambridge University Press;
    [Google Scholar]
  17. Wainwright S.D. 1950 Ph.D. Thesis, London University:
  18. Wainwright S.D., Pollock M.R. 1949; Enzyme adaptation in bacteria: Fate of nitratase in nitrate-adapted cells grown in the absence of substrate. . Brit. J. exp. Path. 30:190
    [Google Scholar]
  19. Wisseman C.L. Jun. Smadel J.E., Hahn F.E., Hopfs H.E. 1954; Mode of action of chloramphenicol. I. Action of chloramphenicol on assimilation of ammonia and on synthesis of proteins and nucleic acids in Escherichia coli. J. Bact. 67:662
    [Google Scholar]
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