1887

Abstract

SUMMARY: By extracting the capsular material of M with hot water, -glutamic acid polypeptide and a polysaccharide were isolated. The polypeptide absorbs the antibody from an anthrax immune serum, which reveals a homogenously distributed capsular material in M. The polysaccharide absorbs one of the homologous antibodies, which renders a characteristic structure visible in the capsule and gives a specific cell-wall reaction. By using lysozyme digestion for the dissolution of the capsule or of the cell-wall a mucoprotein was isolated. The mucoprotein gives serological reactions identical with those of the polysaccharide, and it corresponds to a less degraded form of the cellular substance participating in building up both the cell wall and the capsular structure.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-14-1-14
1956-02-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/14/1/mic-14-1-14.html?itemId=/content/journal/micro/10.1099/00221287-14-1-14&mimeType=html&fmt=ahah

References

  1. Alderton G., Fevold H.L. 1946; Direct crystallization of lysozyme from egg white and some crystalline salts of lysozyme. J. biol. Chem. 164:1
    [Google Scholar]
  2. Aubert J.P. 1949; De l’existence d’un polyoside chez Bac. megatherium. C.R. Acad. Sci. Paris 229:477
    [Google Scholar]
  3. Epstein L.A., Chain E. 1940; Some observations on the preparation and properties of the substrate of lysozyme. Brit. J. exp. Path. 21:339
    [Google Scholar]
  4. Feiner R.R., Meyer K., Steinberg A. 1946; Bacterial lysis by lysozyme. J. Bact. 52:375
    [Google Scholar]
  5. Fiske C.H., Subbarow Y. 1925; The colorimetric determination of phosphorus. J. biol. Chem. 66:375
    [Google Scholar]
  6. Folin O., Wu H. 1920; A simplified and improved method for determination of sugar. J. biol. Chem. 41:367
    [Google Scholar]
  7. Freudenberg K., Weber E. 1925; Mikroacetylbestimmung. Angew. Chem. 38:280
    [Google Scholar]
  8. Hanby W.E., Rydon H.N. 1946; The capsular substance of Bacillus anthracis. Biochem.J. 40:297
    [Google Scholar]
  9. Ivanovics G., Brückner G. 1937; Chemische und immunologische Studien über den Meehanismus der Milzbrandinfektion und Immunität. Z. Immun-Forsch. 90:304
    [Google Scholar]
  10. Koch F.C., McMeekin P.L. 1924; A new direct nesslerisationmicrokjeldahl method and a modification of the Nessler-Folin reagent for ammonia. J. Amer. chem. Soc. 46:2066
    [Google Scholar]
  11. Meyer K., Palmer J.W., Thompson R., Khorazo D. 1936; On the mechanism of lysozyme action. J. biol. Chem. 113:479
    [Google Scholar]
  12. Salton M.R.J. 1952a; Cell wall of Micrococcus lysodeikticus as the substrate of lysozyme. Nature; Lond.: 170746
    [Google Scholar]
  13. Salton M.R.J. 1952b; Studies of the bacterial cell wall. III. Preliminary investigation of the chemical constitution of the cell wall of Streptococcus faecalis Biochim. biophys. Acta 8:510
    [Google Scholar]
  14. Salton M.R.J. 1953; Studies of the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim. biophys. Acta 10:512
    [Google Scholar]
  15. Sevag M.G. 1951 Immuno-catalysis. Springfield, Illinois, U.S.A.: Charles C. Thomas;
    [Google Scholar]
  16. Strange R.E., Powell J.F. 1954; Hexosamine-containing peptides in spores of Bacillus subtilis, B. megatherium and B. cereus. Biochem.J. 58:80
    [Google Scholar]
  17. Tomcsik J. 1951; Complex structure of the bacterial capsule in the genus Bacillus. Experientia 7:459
    [Google Scholar]
  18. Tomcsik J., Guex-Holzer S. 1951; Anthrax-Polypeptid und anderespezies-spezifischeSubstanzen der Kapsel in der Bazillus-Gruppe. Schweiz. Z. Path. 14:515
    [Google Scholar]
  19. Tomcsik J., Guex-Holzer S. 1952; Änderung der Struktur der Bakterienzelleim Verlauf der Lysozym-Einwirkung. Schweiz. Z. Path. 15:517
    [Google Scholar]
  20. Tomcsik J., Guex-Holzer S. 1954a; Demonstration of the bacterial capsule by means of a pH-dependent, salt-like combination with proteins. J. gen. Microbiol. 10:97
    [Google Scholar]
  21. Tomcsik J., Guex-Holzer S. 1954b; A specific cell wall reaction in Bacillus sp. J. gen. Microbiol. 10:317
    [Google Scholar]
  22. Tomcsik J., Guex-Holzer S. 1954c; Genese der komplexenKapselstruktur. Schweiz. Z. Path. 17:221
    [Google Scholar]
  23. Tomcsik J., Guex-Holzer S. 1954d; Antikörperproduktion mit isolierter Bakterienzellwand und mit Protoplasten. Experientia 10:484
    [Google Scholar]
  24. Tomcsik J., Szongott H. 1933; Übereinspezifisches Protein der Kapsel des Milzbrandbazillus. Z. ImmunForsch. 77:86
    [Google Scholar]
  25. Webb M. 1948; The action of lysozyme on heat-killed Gram-positive microorganisms. J. gen. Microbiol. 2:260
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-14-1-14
Loading
/content/journal/micro/10.1099/00221287-14-1-14
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error