1887

Abstract

SUMMARY: in a motile Gram-negative bacterium that can adapt its flagellation to different environments. Cells growing in a liquid culture possess only a single polar flagellum; growth on a solid surface additionally induces multiple lateral flagella. The polar flagellum is primarily used for swimming, i.e. locomotion of the bacterium in a liquid environment, whereas the lateral flagella allow the bacteria to swarm over a solid surface. We have previously described a completely non-motile mutant (Sp7 p90D084), and shown that this mutant has a drastically reduced adsorption capacity to wheat roots. In the present work, we present several lines of evidence demonstrating that adsorption to wheat roots is mediated by the polar flagellum of First, the non-adsorbing mutant Sp7 p90D084 forms no polar and no lateral flagella, but is otherwise undistinguishable from wild-type Second, disintegration of the flagella by heat or acid eliminates adsorption. Third, using a polyclonal antiserum against the polar flagellum filament protein (Fla1), we have isolated out of a collection of 3000 Tn5-B30-induced mutants, three additional and genetically different non-flagellate mutants. Like Sp7 p90D084, these mutants show a severely reduced adsorption capacity to wheat roots. Finally, purified polar flagella bind to wheat roots

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-9-2261
1993-09-01
2019-10-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-9-2261
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error