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Microbiology Society logo Microbiology

Volume 139, Issue 9

Salt-stimulation of caesium accumulation in the euryhaline green microalga Chlorella salina: potential relevance to the development of a biological Cs-removal process Free

Abstract

SUMMARY: Accumulation of Cs by salina was 28-fold greater in cells incubated in the presence than in the absence of 0.5 M-NaCl. An approximate 70% removal of external Cs resulted after 15 h incubation of cells with 50 μ;M-CsCl and 0.5 M-NaCl. LiCl also had a stimulatory effect on Cs uptake, although mannitol did not. Cs influx increased with increasing external NaCl concentration and was maximal between 25-500 mM-NaCl at approximately 4 nmol Cs h (10 cells). Little effect on Cs uptake resulted from the presence of Mg or Ca or from varying the external pH, and Cs was relatively non-toxic towards At increasing cell densities (from 4 × 10 to 1 × 10 cells ml), decreasing amounts of Cs were accumulated per cell although the rate of Cs removal from the external medium was still greatest at the higher cell densities examined. Freely suspended and cell-loaded alginate microbeads accumulated similar levels of Cs, however, 46% of total Cs uptake was attributable to the calcium-alginate matrix in the latter case. When Cs-loaded cells were subjected to hypoosmotic shock, loss of cellular Cs occurred allowing easy Cs recovery. This loss exceeded 90% of cellular Cs when cells were washed with solutions containing ≤ 50 m-NaCl between consecutive Cs uptake periods; these cells subsequently lost their ability to accumulate large amounts of Cs. Maximal Cs uptake (approximately 85.1% removal after three 15 h incubations) occurred when cells were washed with a solution containing 500 m-NaCl and 200 m-KCl between incubations. The relevance of these results to the possible use of in a salt-dependent biological Cs-removal process is discussed.

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© Society for General Microbiology 1993
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1993-09-01
2025-01-14
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Salt-stimulation of caesium accumulation in the euryhaline green microalga Chlorella salina: potential relevance to the development of a biological Cs-removal process
Microbiology 139, 2239 (1993); https://doi.org/10.1099/00221287-139-9-2239
/content/journal/micro/10.1099/00221287-139-9-2239
/content/journal/micro/10.1099/00221287-139-9-2239
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