1887

Microbiology Society logo

Volume 139, Issue 9

Salt-stimulation of caesium accumulation in the euryhaline green microalga Chlorella salina: potential relevance to the development of a biological Cs-removal process Free

Abstract

SUMMARY: Accumulation of Cs by salina was 28-fold greater in cells incubated in the presence than in the absence of 0.5 M-NaCl. An approximate 70% removal of external Cs resulted after 15 h incubation of cells with 50 μ;M-CsCl and 0.5 M-NaCl. LiCl also had a stimulatory effect on Cs uptake, although mannitol did not. Cs influx increased with increasing external NaCl concentration and was maximal between 25-500 mM-NaCl at approximately 4 nmol Cs h (10 cells). Little effect on Cs uptake resulted from the presence of Mg or Ca or from varying the external pH, and Cs was relatively non-toxic towards At increasing cell densities (from 4 × 10 to 1 × 10 cells ml), decreasing amounts of Cs were accumulated per cell although the rate of Cs removal from the external medium was still greatest at the higher cell densities examined. Freely suspended and cell-loaded alginate microbeads accumulated similar levels of Cs, however, 46% of total Cs uptake was attributable to the calcium-alginate matrix in the latter case. When Cs-loaded cells were subjected to hypoosmotic shock, loss of cellular Cs occurred allowing easy Cs recovery. This loss exceeded 90% of cellular Cs when cells were washed with solutions containing ≤ 50 m-NaCl between consecutive Cs uptake periods; these cells subsequently lost their ability to accumulate large amounts of Cs. Maximal Cs uptake (approximately 85.1% removal after three 15 h incubations) occurred when cells were washed with a solution containing 500 m-NaCl and 200 m-KCl between incubations. The relevance of these results to the possible use of in a salt-dependent biological Cs-removal process is discussed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1993
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-9-2239
1993-09-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/9/mic-139-9-2239.html?itemId=/content/journal/micro/10.1099/00221287-139-9-2239&mimeType=html&fmt=ahah

References

  1. Avery S.V., Tobin J.M. 1992; Mechanisms of strontium uptake by laboratory and brewing strains of Saccharomyces cerevisiae.. Applied and Environmental Microbiology 58:3883–3889
     [Google Scholar]
  2. Avery S.V., Codd G.A., Gadd G.M. 1991; Caesium accumulation and interactions with other monovalent cations in the cyanobacterium SynechocystisPCC 6803.. Journal of General Microbiology 137:405–413
     [Google Scholar]
  3. Avery S.V., Codd G.A., Gadd G.M. 1992a; Interactions of cyanobacteria and microalgae with caesium.. In Impact off Heavy Metals on the Environment pp. 133–182, Edited by. Vernet J.-P. Amsterdam:: Elsevier.;
     [Google Scholar]
  4. Avery S.V., Codd G.A., Gadd G.M. 1992b; Replacement of cellular potassium by caesium in Chlorella emersonii:differential sensitivity of photoautotrophic and chemoheterotrophic growth.. Journal of General Microbiology 138:69–76
     [Google Scholar]
  5. Avery S.V., Codd G.A., Gadd G.M. 1993; Transport kinetics, cation inhibition and intracellular location of accumulated caesium in the green microalga Chlorella salina.. Journal of General Microbiology 139:827–834
     [Google Scholar]
  6. Blackwell J.R., Gilmour D.J. 1991; Physiological response of the unicellular green alga Chlorococcum submarinumto rapid changes in salinity.. Archives of Microbiology 157:86–9l
     [Google Scholar]
  7. Brierley J.A., Goyak G.M., Brierley C.L. 1986; Considerations for commercial use of natural products for metal recovery.. In Immobilization of Ions by Biosorption pp. 105–117, Edited by. Eccles H., Hunt S. Chichester:: Ellis Horwood.;
     [Google Scholar]
  8. Brown L.M., Hellebust J.A. 1978; Ionic dependence of deplasmolysis in the euryhaline diatom Cyclotella cryptica.. Canadian Journal of Microbiology 56:408–412
     [Google Scholar]
  9. Ehrenfeld J., Cousin J.-L. 1982; Ionic regulation of the unicellular green alga Dunaliella tertiolecta.. Journal of Membrane Biology 70:47–57
     [Google Scholar]
  10. Ehrenfeld J., Cousin J.-L. 1984; Ionic regulation of the unicellular green alga Dunaliella tertiolecta:response to hypertonic shock.. Journal of Membrane Biology 77:45–55
     [Google Scholar]
  11. Gadd G.M. 1990; Fungi and yeasts for metal accumulation.. In Microbial Mineral Recovery pp. 249–275, Edited by. Ehrlich H.L., Brierley C.L., Brierley J.A. NewYork:: McGraw-Hill.;
     [Google Scholar]
  12. Gadd G.M. 1992; Microbial control of heavy metal pollution.. In Microbial Control of Pollution pp. 59–88, Edited by. Fry J.C., Gadd G.M., Herbert >R.A., Jones C.W., Watson-Craik I. Cambridge:: Cambridge University Press.;
     [Google Scholar]
  13. Garnham G.W., Codd G.A., Gadd G.M. 1991; Effect of salinity and pH on cobalt biosorption by the estuarine microalga Chlorella salina.. Biology of Metals 4:151–157
     [Google Scholar]
  14. Garnham G.W., Codd G.A., Gadd G.M. 1992; Uptake of cobalt, zinc and manganese by the estuarine green microalga Chlorella salina immobilized in alginate microbeads.. Environmental Science and Technology 26:1764–1770
     [Google Scholar]
  15. Gilmour D. 1990; Halotolerant and halophilic microorganisms.. In Microbiology of Extreme Environments pp. 147–177, Edited by. Edwards C. Milton Keynes:: Open University Press.;
     [Google Scholar]
  16. Ginzburg M., Ginzburg B.-Z. l986; The osmotic components of halotolerant algae: unanswered questions.. Trends in Biochemial Sciences 11:359–360
     [Google Scholar]
  17. Macaskie L.E. 1991; The application of biotechnology to the treatment of wastes produced from the nuclear fuel cycle: biodegradation and bioaccumulation as a means of treating radionuclide-containing streams.. Critical Reviews in Biotechnology 11:41–112
     [Google Scholar]
  18. Meikle A.J., Gadd G.M., Reed R.H. 1990; Manipulation of yeast for transport studies: critical assessment of cultural and experimental procedures.. Enzyme and Microbial Technology 12:865–872
     [Google Scholar]
  19. Pick U., Ben-Amotz A., Karni L., Seebergts C.J., Avron M. 1986; Partial characterization of K+ and Ca2+ uptake systems in the halotolerant alga Dunaliella salina.. Plant Physiology 81:875–881
     [Google Scholar]
  20. Plato P., Denovan J.T. 1974; The influence of potassium on the removal of 137Cs by live Chlorellafrom low level radioactive wastes.. Radiation Botany 14:37–41
     [Google Scholar]
  21. Springer-Lederer H., Rosenfeld D.L. 1968; Energy sources for the absorption of rubidium by Chlorella.. Physiologia Plantarum 21:435–444
     [Google Scholar]
  22. Tomioka N., Uchiyama H., Yagi 0. 1992; Isolation and characterization of cesium-accumulating bacteria.. Applied and Environmental Microbiology 58:1019–1023
     [Google Scholar]
  23. Walderhaug M.O., Dosch D.C., Epstein W. 1987; Potassium transport in bacteria.. In Ion Transport in Prokaryotes pp. 85–130, Edited by. Rosen B.P., Silver S. NewYork:: Academic Press.;
     [Google Scholar]
  24. Wong K.H., Chan K.Y., Ng S.L. 1979; Cadmium uptake by the unicellular green alga Chlorella salinaCU-1 from culture media with high salinity.. Chemosphere 11/12:887–891
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-9-2239
Loading
Salt-stimulation of caesium accumulation in the euryhaline green microalga Chlorella salina: potential relevance to the development of a biological Cs-removal process
Microbiology 139, 2239 (1993); https://doi.org/10.1099/00221287-139-9-2239
/content/journal/micro/10.1099/00221287-139-9-2239
/content/journal/micro/10.1099/00221287-139-9-2239
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error