SUMMARY: The closely related strains BR, S21 and Thr, isolated from the genus , Allo2 and Dec from the genus , and G80 from the genus , could be distinguished by their esterase zymograms after ultra-low gelling point agarose-polyacrylamide electrophoresis. The kanamycin-resistant derivatives S21-kR and BR-kR could also be differentiated from their kanamycin-sensitive parental strains. Different patterns of intracellular esterases could be obtained by using β-naphthyl propionate or 3-indoxyl acetate as substrates. The substrate 5-bromo-4-chloro-3-indoxyl acetate proved particularly useful, revealing a variety of esterases from isolates. Zymograms of aminopeptidases from all strains were found to be quite similar. Nevertheless, they allowed differentiation of strains BR and S21 from their kanamycin-resistant derivatives. Aminopeptidase and esterase zymograms obtained from isolates were markedly different from all others. Zymograms of esterases and aminopeptidases may prove useful for the identification of some other strains.


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