1887

Abstract

SUMMARY: The open reading frame of the OprI lipoprotein gene from was amplified by polymerase chain reaction (PCR) starting from purified DNA or colony lysates. A fragment of the expected size (249 bp) was detected in all strains from various clinical and geographical origins. The gene could only be amplified in pseudomonads of rRNA group I which are considered to be the authentic genus Digestions with III, II and of the amplified fragments demonstrated a sequence variation in the gene. Colony, dot and Western blots with two monoclonal antibodies (mAbs) against the lipoprotein I confirmed our PCR results. These findings open interesting perspectives for the molecular taxonomy of the genus and the development of diagnostic tools.

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1993-09-01
2021-10-20
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References

  1. Bodey G.P., Bolivar R., Fainstein V., Jadeja L. 1983; Infections caused by Pseudomonas aeruginosa.. Reviews of Infectious Diseases 5:279–313
    [Google Scholar]
  2. Bodey G.P., Jadeja L., Elting L. 1985; Pseudomonasbacteremia: retrospective analysis of 410 episodes.. Archives of Internal Medicine 145:1621–1629
    [Google Scholar]
  3. Botzenhart K., RÜden H. 1987; Hospital infections caused by Pseudomonas aeruginosa.. Antibiotics and Chemotherapy 39:1–15
    [Google Scholar]
  4. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.. Analytical Biochemistry 76:248–254
    [Google Scholar]
  5. Cornelis P., Bouia A., Guyonvarch A., Kammerer B., Hannaert V., Hubert J.C. 1989; Cloning and analysis of the gene for the major outer membrane lipoprotein from Pseudomonas aeruginosa.. Molecular Microbiology 3:421–428
    [Google Scholar]
  6. Davison J. 1988; Plant beneficial bacteria.. Bio/Technology 6:282–286
    [Google Scholar]
  7. De Vos P., De Ley J. 1983; Intra- and intergeneric similarities of Pseudomonasand Xanthomonasribosomal ribonucleic acid cistrons.. International Journal of Systematic Bacteriology 33:487–509
    [Google Scholar]
  8. De Vos P., Goor M., Gillis M., De Ley J. 1985; Ribosomal ribonucleic acid cistron similarities of phytopathogenic Pseudomonasspecies.. International Journal of Systematic Bacteriology 35:169–184
    [Google Scholar]
  9. De Vos P., Van Landschoot A., Segers P., Tytgat R., Gillis M., Bauwens M., Rossau R., Goor M., Pot B., Kersters K., Lizzaraga P., De Ley J. 1989; Genotypic relationships and taxonomic localization of unclassified Pseudomonasand Pseudomonas-like strains by deoxyribonucleic acid: ribosomal ribonucleic acid hybridizations.. International Journal of Systematic Bacteriology 39:35–49
    [Google Scholar]
  10. DuchÊne M., Barron C., Schweizer A., Von Specht B.U., Domdey H. 1989; Pseudomonas aeruginosaouter membrane lipoprotein I gene: molecular cloning, sequence, and expression in Escherichia coli.. Journal of Bacterialogy 171:4l30–4137
    [Google Scholar]
  11. Filip C., Fletcher G., Wulff J.L., Earhart C.F. 1973; Solubilisation of the cytoplasmic membrane of Escherichia coliby the ionic detergent sodium-lauryl-sarcosinate.. Journal of Bacteriology 115:717–722
    [Google Scholar]
  12. Finke M., Muth G., Reichelm T., Thoma M., DuchÊne M., Hungerer K.D., Domdey H., Von Specht B.U. 1991; Protection of immunosuppressed mice against infection with Pseudomonas aeruginosaby recombinant P. aeruginosalipoprotein I and lipoprotein I-specific monoclonal antibodies.. Infection and Immunity 59:1251–1254
    [Google Scholar]
  13. Govan J.R.W. 1986; Pseudomonas aeruginosaand cystic fibrosis: unusual bacterial adaptation and pathogenesis.. Microbiological Science 3:302–308
    [Google Scholar]
  14. Hoiby N., DÖring G., Schiotz P.O. 1987; Pathogenic mechanisms of chronic Pseudomonas aeruginosainfections in cystic fibrosis patients.. Antibiotics and Chemotherapy 39:60–76
    [Google Scholar]
  15. Holmdahl R., Moran T., Andersson M. 1985; A rapid and efficient immunization protocol for production of monoclonal antibodies reactive with autoantigens.. Journal nf Immunological Methods 83:379–384
    [Google Scholar]
  16. Mondino B.J., Weissman B.A., Farb M.D., Pettit T.H. 1986; Corneal ulcers associated with daily wear and extended wear contact lenses.. American Journal of Ophthaltnology 102:58–65
    [Google Scholar]
  17. Palleroni N.J. 1984; Genus I. PseudomonasMigula 1894.. In Bergey̓s Manual of Systematic Bacteriology 1 pp. 141–199, Edited by. Krieg N.R., Holt J.G. Baltimore:: Williams & Wilkins.;
    [Google Scholar]
  18. Palleroni N.J., Ballard R.W., Ralston E., Doudoroff M. 1972; Deoxyribonucleic acid homologies among some Pseudomonasspecies.. Journal of Bacteriology 110:1–11
    [Google Scholar]
  19. Reinhold-Hurek B., Hurek T., Gillis M., Hoste B., Vancanneyt M., Kersters K., De Lay J. 1993; Azoarcusgen. nov., nitrogen-fixing Proteobacteria associated with roots of Kallar Grass (Leptochloa fusca(L.) Kunth), and description of two species Azoarcus indigenssp. nov. and Azoarcus commenissp. nov.. International Journal of Systematic Bacteriology 43:574–584
    [Google Scholar]
  20. Saint-Onge A., Romeyer F., Lebel P., Masson L., Brousseau R. 1992; Specificity of the PseudomonasPAO1 lipoprotein 1 gene as a DNA probe and PCR target region within the Pseudomonadaceae.. Journal of General Microbiology 138:733–741
    [Google Scholar]
  21. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning, a Laboratory Manual. Cold Spring Harbor, NY :: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  22. Siehnel R., Martin N.L., Hancock R.E.W. 1990; Sequence and relatedness in other bacteria of the Pseudomonas aeruginosaOprP gene coding for the phosphate-specific porin P.. Molecular Microbiology 4:831–838
    [Google Scholar]
  23. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications.. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  24. Ullstrom C.A., Siehnel R., Woodruff W., Steinbach S., Hancock R.E. 1991; Conservation of the gene for outer membrane protein OprF in the family Pseudomonadaceae:sequence of the Pseudomonas syringaeOprF gene.. Journal of Barteriology 173:768–775
    [Google Scholar]
  25. Weller D.M. 1988; Biological control of soilborne plant pathogens in the rhizosphere with bacteria.. Annual Review of Phytopathology 26:379–407
    [Google Scholar]
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