1887

Abstract

SUMMARY: HPLC analysis revealed that WA-C produced two substances under iron-limiting conditions one of which was identified as 2,3-dihydroxybenzoyl-L-serine. The other compound had iron-complexing activity and was called yersiniabactin. The mutant H1852 was shown to produce yersiniabactin constitutively in an iron-independent manner. Yersiniabactin was isolated by ethyl acetate extraction from the spent medium of H1852, size-fractionation chromatography and preparative HPLC. A catechol function was demonstrated with different chemical assays and by UV-visible spectroscopy. The molecular mass of yersiniabactin was determined to be 482 Da. Purified yersiniabactin stimulated growth of and coli Ø under iron-limiting conditions and apparently served as an iron carrier. Transport of Fe-yersiniabactin was TonB-dependent, indicating a receptor-mediated uptake across the outer membrane. A pesticin-resistant mutant missing the receptor protein FyuA was unable to transport and use yersiniabactin as a siderophore.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-9-2159
1993-09-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/9/mic-139-9-2159.html?itemId=/content/journal/micro/10.1099/00221287-139-9-2159&mimeType=html&fmt=ahah

References

  1. Aharonowitz Y. 1980; Nitrogen metabolite regulation of antibiotic biosynthesis.. Annual Review of Microbiology 34:209–233
    [Google Scholar]
  2. Arnow L.E. 1937; Colorimetric determination of the components of 3,4-dihydroxy-phenylalanine-tvrosine mixtures.. Journal of Biological Chemistry 118:531–537
    [Google Scholar]
  3. BÄumler A., Koebnik R., Stojiljkovic I., Heesemann J., Braun V., Hantke K. 1993; Survey on newly characterized iron uptake systems of Yersinia enterocolitica. . Zentralblatt für Mikrobiologie 278:416–424
    [Google Scholar]
  4. Braun V., Hantke K. 1991; Genetics of bacterial iron transport.. In Handbook of Bacterial Iron Chelates pp. 107–183 Winkelmann G. Edited by Boca Raton: CRC Press.;
    [Google Scholar]
  5. Cove J.H., Holland K.T., Cunliff W.J. 1980; The vitamin requirements of staphylococci isolated from the human skin.. Journal of Applied Bacteriology 49:29–37
    [Google Scholar]
  6. Crosa J.H. 1989; Genetics and molecular biology of siderophore-mediated iron transport in bacteria.. Microbiological Reviews 53:517–530
    [Google Scholar]
  7. Czaky T.Z. 1948; On the estimation of bound hydroxylamine in biological materials.. Acta Chemica Scandinavica 2:450–454
    [Google Scholar]
  8. Ferber D.M, Brubacker R.R. 1981; Mutations to tolerance and resistance to pesticin and colicins in Escherichia coli ɸ. . Journal of Bacteriology 146:506–511
    [Google Scholar]
  9. Gaden E.L. 1959; Fermentation process kinetics.. Journal of Biochemical and Microbiological Techniques and Engineering 1:413–442
    [Google Scholar]
  10. Heesemann J. 1987; Chromosomal encoded siderophores are required for mouse virulence of enteropathogenic Yersinia species.. FEMS Microbiology Letters 48:229–233
    [Google Scholar]
  11. Heesemann J., Vocke T., Hantke K., Saken E., Stojiljkovic I., Rakin A., Berner R. 1993; Virulence of Yersinia enterocoliticais closely associated with siderophore production, expression of an iron repressible outer membrane polypeptide of 65000 daltons and pesticin-sensitivity.. Molecular Microbiology 8:397–408
    [Google Scholar]
  12. Koebnik R., BÄumler A., Heesemann J., Braun V., Hantke K. 1993; The TonB protein of Yersinia enterocolitica and its interactions with TonB-box proteins.. Molecular and General Genetics 237:152–160
    [Google Scholar]
  13. Miles A.A., Khimji P.L. 1975; Enterobacteria] chelators of iron: their occurrence, detection, and relation to pathogenicity.. Journal of Medical Microbiology 8:477–490
    [Google Scholar]
  14. Miller J.H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  15. Perry R.D., Brubaker R.R. 1979; Accumulation of iron by Yersiniae. . Journal of Bacteriology 137:1290–1298
    [Google Scholar]
  16. Plaga A., StÜmpfel J., Fiedler H.P. 1989; Determination of carbohydrates in fermentation processes by high performance liquid chromatography.. Applied Microbiology and Biotechnology 32:45–49
    [Google Scholar]
  17. Rioux C, Jordan D.C., Rattray J.B.M. 1983; Colorimetric determination of catechol siderophores in microbial cultures.. Analytical Biochemistry 133:163–169
    [Google Scholar]
  18. Robins-Browne R.M., Prpic J.K. 1985; Effects of iron and desferrioxamine on infections with Yersinia enterocolitica. . Infection and Immunity 47:779–794
    [Google Scholar]
  19. Rogers H.J. 1973; Iron-binding catechols and virulence in Escherichia coli. . Infection and Immunity 7:445–456
    [Google Scholar]
  20. Schwyn B., Neilands J.B. 1987; Universal chemical assay for the detection and determination of siderophores.. Analytical Biochemistry 160:47–56
    [Google Scholar]
  21. Staggs T.M., Perry R.D. 1992; Fur regulation in Yersinia species.. Molecular Microbiology 6:2507–2516
    [Google Scholar]
  22. Stojiljkovic I., Hantke K. 1992; Hemin uptake of Yersinia enterocolitica-. similarities with other Ton B dependent systems in gram-negative bacteria.. EMBO Journal 11:4359–4367
    [Google Scholar]
  23. Stuart S., Prpic J.K., Robins-Browne R.M. 1986; Production of aerobactin by some species of the genus Yersinia. . Journal of Bacteriology 166:1131–1133
    [Google Scholar]
  24. Wake A., Misawara M., Matsui A. 1975; Siderochrome production in Yersinia pestis and its relation to virulence.. Infection and Immunity 12:1211–1213
    [Google Scholar]
  25. Weinberg E.D. 1984; Iron and infection.. Microbiological Reviews 42:45–66
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-9-2159
Loading
/content/journal/micro/10.1099/00221287-139-9-2159
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error