Molecular analysis of a gene encoding a serum-resistance-associated 76 kDa surface antigen of Haemophilus somnus Free

Abstract

SUMMARY: is a Gram-negative bacterial bovine pathogen which can cause disease or be carried asymptomatically. We previously showed that four serum-sensitive isolates from asymptomatic carriers lacked a 13.4 kb sequence of chromosomal DNA that was present in two virulent serum-resistant strains. We have since sequenced 5 kb of the 13.4 kb fragment from a serum-resistant strain, which contained an open reading frame (ORF) of at least 4.5 kb. From Western blot analysis, the ORF was shown to encode a 76 kDa protein (p76) that co-migrated with a 76 kDa surface protein. Both the recombinant and natural p76 reacted with convalescent-phase serum from a cow in an experimental abortion study. The translational start site for p76 was identified by deletion analysis of subclones of the 5 kb cloned sequence. The 4.5 kb ORF contained 1.2 kb tandem direct repeats (DRs), with 65% identity between the two repeats at the protein level. The 5' DR (DR1) included the start site for the 76 kDa protein, and DR2 had a flanking inverted repeat, suggestive of an insertion-sequence-like element.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-9-2135
1993-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/9/mic-139-9-2135.html?itemId=/content/journal/micro/10.1099/00221287-139-9-2135&mimeType=html&fmt=ahah

References

  1. Bailie W.E., Coles E.H., Weide K.D. 1973; Deoxyribonucleic acid characterization of a microorganism isolated from infectious thromboembolic meningoencephalomyelitis of cattle.. International Journal of Systematic Bacteriology 23:231–237
    [Google Scholar]
  2. Barbour A.G., Carter C.J., Burman N., Freitag C.S., Garon C.F., Bergstrom S. 1991; Tandem insertion sequence-like elements define the expression site for variable antigen genes of Borrelia hermsii. . Infection and Immunity 59:390–397
    [Google Scholar]
  3. Cole S.P., Guiney D.G., Corbeil L.B. 1992; Two linked genes for outer membrane proteins are absent in four non-disease strains of Haemophilus somnus. . Molecular Microbiology 6:1895–1902
    [Google Scholar]
  4. Corbeil L.B., Blau K., Prieur D.J., Ward A.C.S. 1985; Serum susceptibility of Haemophilus somnus from bovine clinical cases and carriers.. Journal of Clinical Microbiology 22:192–198
    [Google Scholar]
  5. Corbeil L.B., Chikami G., Yarnall M., Smith J., Guiney D.G. 1988; Cloning and expression of genes encoding Haemophilus somnus antigens.. Infection and Immunity 56:2736–2742
    [Google Scholar]
  6. Dunn J.J, Studier F.W. 1983; Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.. Journal of Molecular Biology 166:477–535
    [Google Scholar]
  7. Eisenberg S., Finer M. 1980; Cleavage and circularization of single-stranded DNA: a novel enzymatic activity of ɸX174 A* protein.. Nucleic Acids Research 22:5305–5315
    [Google Scholar]
  8. Fulford W., Model P. 1988; Regulation of bacteriophage fl DNA replication. I. New functions for genes II and X. . Journal of Molecular Biology 203:49–62
    [Google Scholar]
  9. Gilmore R.D. JR Joste N., Mcdonald G.A. 1989; Cloning, expression and sequence analysis of the gene encoding the 120 kDa surface-exposed protein of Rickettsia rickettsii. . Molecular Microbiology 3:1579–1586
    [Google Scholar]
  10. Gilmore R.D. JR Cieplak N. JR Policastro P.F., Hackstadt T. 1991; The 120 kilodalton outer membrane protein (rOmpB) of Rickettsia rickettsii is encoded by an unusually long open reading frame: evidence for protein processing from a large precursor.. Molecular Microbiology 5:2361–2370
    [Google Scholar]
  11. Gogolewski R.P., Kania S.A., Inzana T.J., Widders P.R., Liggitt H.D., Corbeil L.B. 1987; Protective ability and specificity of convalescent serum from calves with Haemophilus somnus pneumonia.. Infection and Immunity 55:1403–1411
    [Google Scholar]
  12. Groom S.C., Little P.B., Rosendal S. 1988; Virulence differences among three strains of Haemophilus somnus following intratracheal inoculation of calves.. Canadian Journal of Veterinary Research 52:349–354
    [Google Scholar]
  13. Harris F.W., Janzen E.D. 1989; The Haemophilus somnus disease complex (Hemophilosis): a review.. Canadian Veterinary Journal 30:816–820
    [Google Scholar]
  14. Holmes D.S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids.. Analytical Biochemistry 114:193–197
    [Google Scholar]
  15. Humphrey J.D. 1982 Haemophilus somnus: colonization of the bovine reproductive tract. [As quoted in Groom et al. (1988) and Humphrey & Stephens (1983).] PhD thesis University of Guelph, Ont., Canada.:
    [Google Scholar]
  16. Humphrey J.D., Stephens L.R. 1983; Haemophilus somnus: a review.. Veterinary Bulletin 53:987–1004
    [Google Scholar]
  17. Inzana T.J., Corbeil L.B. 1987; Further characterization of the growth requirements of Haemophilus somnus : development of a defined medium.. American Journal of Veterinary Research 48:366–369
    [Google Scholar]
  18. Inzana T.J. 1992; Immune response of cattle to Haemophilus somnus lipid A-protein conjugate vaccine and efficacy in a mouse abortion model.. American Journal of Veterinary Research 53:175–179
    [Google Scholar]
  19. Isberg R.R., Laazaar A.L., Syvanen M. 1982; Regulation of Tn5 by the right-repeat proteins: control at the level of the transposition reaction.. Cell 30:883–892
    [Google Scholar]
  20. Johnson R.C., Reznikoff W.S. 1984; Role of the IS50R proteins in the promotion and control of Tn5 transposition.. Journal of Molecular Biology 177:645–661
    [Google Scholar]
  21. Kwiecien J.M., Little P.B. 1991; Haemophilus somnus and reproductive disease in the cow: a review.. Canadian Veterinary Journal 32:595–601
    [Google Scholar]
  22. Kyte J., Doolittle R.F. 1982; A simple method for displaying the hydropathic character of a protein.. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  23. Lo R.Y. 1992; An analysis of the codon usage of Pasteurella haemolytica Al.. FEMS Microbiology Letters 19:125–131
    [Google Scholar]
  24. Mills J.A., Venkatesan M.M., Baron L.S., Buysse J.M. 1992; Spontaneous insertion of an ISi-like element into the virF gene is responsible for avirulence in opaque colonial variants of Shigella ftexneri 2a.. Infection and Immunity 60:175–182
    [Google Scholar]
  25. Notarnicola S.M., Mcintosh M.A. 1990; Multiple translational products from a Mycoplasma hyorhinis gene expressed in Escherichia coli. . Journal of Bacteriology 172:2986–2995
    [Google Scholar]
  26. Pritchard A.E., Vasil M.L. 1990; Possible insertion sequences in a mosaic genome organization upstream of the exotoxin A gene in Pseudomonas aeruginosa. . Journal of Bacteriology 172:2020–2028
    [Google Scholar]
  27. Ross T.K., Achberger E.C., Braymer H.D. 1989; Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus.. Journal of Bacteriology 171:1974–1981
    [Google Scholar]
  28. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  29. Sharp P.M., Cowe E., Higgins D.G., Shields D.C., Wolfe K.H., Wright F. 1988; Codon usage patterns in Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Drosophila melanogaster and Homo sapiens; a review of the considerable within-species diversity.. Nucleic Acids Research 16:8207–8211
    [Google Scholar]
  30. Theisen M., Potter A. A. 1992; Cloning, sequencing, expression, and functional studies of a 15000-molecular-weight Haemophilus somnus antigen similar to Escherichia coli ribosomal protein S9.. Journal of Bacteriology 174:17–23
    [Google Scholar]
  31. Theisen M., Rioux C.R., Potter A.A. 1992; Molecular cloning, nucleotide sequence, and characterization of a 40000-molecular-weight lipoprotein of Haemophilus somnus. . Infection and Immunity 60:826–831
    [Google Scholar]
  32. Von Heijne G. 1983; Patterns of amino acids near signal-sequence cleavagesites.. European Journal of Biochemistry 133:17–21
    [Google Scholar]
  33. Widders P.R., Paisley L.G., Gogolewski R.P., Evermann J.F., Smith J.W., Corbeil L.B. 1986; Experimental abortion and the systemic immune response in cattle to Haemophilus somnus. . Infection and Immunity 54:555–560
    [Google Scholar]
  34. Widders P.R., Dorrance L.A., Yarnall M., Corbeil L.B. 1989; Immunoglobulin-binding activity among pathogenic and carrier isolates of Haemophilus somnus. . Infection and Immunity 57:639–642
    [Google Scholar]
  35. Yarnall M., Gogolewski R. P., Corbeil L. B. 1988a; Characterization of two Haemophilus somnus Fc receptors.. Journal of General Microbiology 134:1993–1999
    [Google Scholar]
  36. Yarnall M., Widders P. R., Corbeil L.B. 1988b; Isolation and characterization of Fc receptors from Haemophilus somnus. . Scandinavian Journal of Immunology 28:129–137
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-9-2135
Loading
/content/journal/micro/10.1099/00221287-139-9-2135
Loading

Data & Media loading...

Most cited Most Cited RSS feed