1887

Abstract

SUMMARY: Three isolates of showing differing sensitivities to phosphonate were grown in the presence of a uniform concentration of the anion (1 mM), together with concentrations of phosphate which varied from zero to 3 mM. The phosphonate-sensitive isolate was inhibited by phosphonate at all levels of phosphate, but the resistant isolates were inhibited by phosphonate only when phosphate was limiting to growth and were able to exclude phosphonate more effectively than the sensitive isolate at higher concentrations of phosphate. The percentage inhibition in each strain was proportional to the internal concentration of phosphonate, but the slope of the plot of inhibition against internal phosphonate varied between strains. Differences in the capacity to discriminate between phosphate and phosphonate therefore provide part of, but not the whole explanation for differences in sensitivity between isolates. There was a significant increase in the internal concentration of orthophosphate in the mycelia which were inhibited by phosphonate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-9-2109
1993-09-01
2021-04-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/9/mic-139-9-2109.html?itemId=/content/journal/micro/10.1099/00221287-139-9-2109&mimeType=html&fmt=ahah

References

  1. Barchietto T., Saindrenan P., Bompeix G. 1988; Uptake and utilisation of phosphite by Phytophthora citrophthora and Nectria haemutococca in relation to its selective toxicity. Pesticide . . Science 22:54–58
    [Google Scholar]
  2. Barchietto T., Saindrenan P., Bompfix G. 1989; Characterisation of phosphonate uptake in two Phytophthora spp. and its inhibition by phosphate.. Archives of Microbiology 151:54–58
    [Google Scholar]
  3. Barchietto T., Saindrenan P., Bompeix G. 1992; Physiological responses of Phytophthora citrophthora to a sub-inhibitory concentration of phosphonate.. Pesticide Chemistry and Physiology 42:151–166
    [Google Scholar]
  4. Bompeix G. 1984; In vitro antifungal activity of Fosetyl Al and phosphorous acid on Phytophthora species.. Fruits 39:777–786
    [Google Scholar]
  5. Caten C.E., Jinks J.L. 1968; Spontaneous variation in single isolates of Phytophthora infestans. 1. Cultural variation.. Canadian Journal of Botany 46:329–348
    [Google Scholar]
  6. Coffey M.D., Outmette D.G. 1989; Phosphonates: Antifungal compounds against Oomycetes.. In Nitrogen, Phosphorus and Sulphur Utilisation by Fungi British Mycological Society Symposium pp 107–129 Boddy L., Marchant R., Read D. J. Edited by Cambridge: Cambridge University Press.;
    [Google Scholar]
  7. Cohen Y., Coffey M.D. 1986; Systemic fungicides and the control of oomycetes.. Annual Review of Phytopathology 24:311–338
    [Google Scholar]
  8. Davis A.J., Say M., Snow A.J., Grant B.R. 1993; Sensitivity of Fusarium oxysporum f. sp. cubense to phosphonate.. Plant Pathology (in the press).
    [Google Scholar]
  9. Dolan T.E., Coffey M.D. 1988; Correlative in vitro and in vivo behaviour of mutant strains of Phytophthora palmivora expressing diiferent resistances to phosphorous acid and fosetyl-Na.. Phyto-pathology 78:974–978
    [Google Scholar]
  10. Dunstan R.H., Smillie R.H., Grant B.R. 1990; The effects of subtoxic levels of phosphonate on the metabolism and potential virulence factors of Phytophthora palmivora. . Physiological and Molecular Plant Pathology 36:205–220
    [Google Scholar]
  11. Fenn M.E., Coffey M.D. 1984; Studies on the in vitro and in vivo antifungal activity of fosetyl-Al and phosphorous acid.. Phytopathology 74:606–611
    [Google Scholar]
  12. Grant B.R., Griffith J.M., Irving H.R., Radda M. 1984; An improved method for the synchronous production of zoospores from Phytophthora palmivora. . Experimental Mycology 8:382–385
    [Google Scholar]
  13. Griffith J.M., Akins L.A., Grant B.R. 1989a Properties of the phosphate and phosphite transport systems of Phytophthora palmivora. . Archives of Microbiology 152:430–436
    [Google Scholar]
  14. Griffith J.M., Smillie R. H., Niere J.O., Grant B.R. 1989b; Effect of phosphate on the toxicity of phosphite in Phytophthora palmivora. . Archives of Microbiology 152:425–429
    [Google Scholar]
  15. Griffith J.M., Smillie R.H., Grant B.R. 1990; Alterations in nucleotide and pyrophosphate levels in Phytophthora palmivora following exposure to the antifungal agent potassium phosphonate (phosphite).. Journal of General Microbiology 136:1285–1291
    [Google Scholar]
  16. Griffith J.M., Davis A.J., Grant B.R. 1992; Target sites of fungicides to control Oomycetes.. In Target Sites of Fungicide Action pp. 69–100 Roller W. Edited by Boca Raton Florida: CRC Press.;
    [Google Scholar]
  17. Guest D., Grant B. 1991; The complex action of phosphonates as antifungal agents.. Biological Reviews 66:159–187
    [Google Scholar]
  18. Heaton J.B., Dullahide S.R. 1990; Efficacy of phosphonic acid in other host pathogenic systems.. Australasian Plant Pathology 19:133–134
    [Google Scholar]
  19. Hendrix J.W. 1965; Influence of sterols on growth and reproduction of Pythium and Phytophthora spp.. Phytopathology 55:790–797
    [Google Scholar]
  20. Hendrix J.W., Apple J.L. 1964; Fats and fatty acid derivatives as growth stimulants and carbon sources for Phytophthora parasitica var nicotianae. . Phytopathology 54:987–994
    [Google Scholar]
  21. Irving H.R., Griffith J.M., Grant B.R. 1984; Calcium efflux associated with encystment of Phytophthora palmivora zoospores.. Cell Calcium 5:487–500
    [Google Scholar]
  22. Niere J.O., Griffith J.M., Grant B.R. 1990; 31P NMR studies on the effect of phosphite on Phytophthora palmivora. . Journal of General Microbiology 136:147–156
    [Google Scholar]
  23. Rawn C.D., Vaneiten J.L. 1974; A shake culture method for Pythiaceae applicable to rapid small scale assay of vegetative physiology.. Phytopathology 68:1384–1388
    [Google Scholar]
  24. Ribeiro O.K., Erwin D.C., Zentmyer G.A. 1975; An improved synthetic medium for oospore production and germination of several Phytophthora species.. Mycologia 67:1012–1019
    [Google Scholar]
  25. Rosenberg H., Lanauze J.M. 1968; Isolation of a mutant of Bacillus cereus deficient in phosphate transport.. Biochimica et Biophysica Acta 156:381–388
    [Google Scholar]
  26. Smillie R.H., Grant B.R., Guest D.I. 1989; The mode of action of phosphite : evidence for both the direct and indirect modes of action on three species of plants.. Phytopathology 79:921–926
    [Google Scholar]
  27. Tokunaga J., Bartnicki-Garcia S. 1971; Cyst wall formation and endogenous carbohydrate utilisation during synchronous encystment of Phytophthora palmivora zoospores.. Archiv für Microbiologie 79:283–292
    [Google Scholar]
  28. Zweck S., HüTterman A., Chet I. 1978; A convenient method of preparing inocula of homogenised mycelia.. Experimental Mycology 2:377–378
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-9-2109
Loading
/content/journal/micro/10.1099/00221287-139-9-2109
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error