1887

Abstract

SUMMARY: As described previously, in sp. (formerly ) strains MR11 and MR22, the ability to grow as an aerobic hydrogen bacterium (the Aut character) is located on giant conjugative linear plasmids - on pHG201 (270 kb) in strain MR11 and on pHG205 (280 kb) in strain MR22. In an autotrophic transconjugant originating from MR22 a smaller plasmid, pHG207 (225 kb), was detected and shown to be a recombination product of the wild-type plasmids pHG204 and pHG205. A donor carrying pHG207 as the sole plasmid transferred the Aut marker at a 1000-fold frequency compared to the wild-type plasmid pHG205. Analysis of the plasmid ends revealed that plasmid pHG207 carries proteins at both ends; the proteins are linked to the 5' ends of the strands. The cloned end fragments of about 2 kb were sequenced and found to contain highly homologous sequences within the terminal 583 bp (left end part) and 560 bp (right end part). Several potential reading frames were detected, but database searching gave no indication about possible functions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-9-2055
1993-09-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/9/mic-139-9-2055.html?itemId=/content/journal/micro/10.1099/00221287-139-9-2055&mimeType=html&fmt=ahah

References

  1. Anand R. 1986; Pulsed field gelelectrophoresis: a technique for fractionating large DNA molecules.. Trends in Genetics 2:278–283
    [Google Scholar]
  2. Ausubel F.M., Brent R., Kingston R.E., More D.D., Seidman J.G., Smith J.A., Struhl K. 1987 Current Protocols in Molecular Biology. New York: J. Wiley and Sons.;
    [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Research 12:387–395
    [Google Scholar]
  4. Ecker C., Reh M., Schlegel H.G. 1986; Enzymes of the autotrophic pathway in mating partners and transconjugants of Nocardia opaca lb and Rhodococcus erythropolis. . Archives of Microbiology 145:280–286
    [Google Scholar]
  5. Fawcett T.W., Bartlett S.G. 1990; An effective method for eliminating ‘artifact banding’ when sequencing double-stranded DNA templates.. BioTechniques 9:46–49
    [Google Scholar]
  6. Hirochika H., Nakamura K., Sakaguchi K. 1984; A linear DNA plasmid from Streptomyces rochei with inverted terminal repetition of 614 base pairs.. EMBO Journal 3:761–766
    [Google Scholar]
  7. Holmes D.S., Quigley M. 1981; A rapid boiling method for preparation of bacterial plasmids.. Analytical Biochemistry 114:193–197
    [Google Scholar]
  8. Ito J. 1978; Bacteriophage Phi29 terminal protein: its association with the 5’ termini of the Phi29 genome.. Journal of Virology 28:895–904
    [Google Scholar]
  9. Kalkus J., Reh M., Schlegel H.G. 1990; Hydrogen autotrophy of Nocardia opaca strains is encoded by linear megaplasmids.. Journal of General Microbiology 136:1145–1151
    [Google Scholar]
  10. Khandjian E.W. 1987; Optimized hybridization of DNA blotted and fixed to nitrocellulose and nylon membranes.. Bio/Technology 5:165–167
    [Google Scholar]
  11. Kinashi H., Shimaji-Murayama M. 1991; Physical character�ization of SCP1, a giant linear plasmid from Streptomyces coelicolor. . Journal of Bacteriology 173:1523–1529
    [Google Scholar]
  12. Kinashi H., Shimaji-Murayama M., Hanafusa T. 1991; Nucleotide sequence analysis of the unusually long terminal inverted repeats of a giant linear plasmid, SCP1.. Plasmid 26:123–130
    [Google Scholar]
  13. Mcclelland M., Jones R., Patel Y. 1987; Restriction endonucleases for pulsed field mapping of bacterial genomes.. Nucleic Acids Research 15:5985–6005
    [Google Scholar]
  14. Marusyk R., Sergeant A. 1980; A simple method for dialysis of small-volume samples.. Analytical Biochemistry 105:403–404
    [Google Scholar]
  15. Oelmüller U., Krüger N., Steinbüchel A., Friedrich C.G. 1990; Isolation of prokaryotic RNA and detection of specific messenger-RNA with biotinylated probes.. Journal of Microbiological Methods 11:72–81
    [Google Scholar]
  16. Reh M. 1981; Chemolithoautotrophy as an autonomous and transferable property of Nocardia opaca lb.. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Abteilung 1, Supplement) 11:577–583
    [Google Scholar]
  17. Reh M., Schlegel H.G. 1975; Chemolithoautotrophie als eine übertragbare, autonome Eigenschaft von Nocardia opaca 1b.. Nachrichten der Akademie der Wissenschaften Güttingen 12:1–10
    [Google Scholar]
  18. Reh M., Schlegel H.G. 1981; Hydrogen autotrophy as a transferable genetic character of Nocardia opaca lb.. Journal of General Microbiology 126:327–336
    [Google Scholar]
  19. Rohe M., Schrage K., Meinhardt F. 1991; The linear plasmid pMC3-2 from Morchella conica is structurally related to adenovirus.. Current Genetics 20:527–533
    [Google Scholar]
  20. Sakaguchi K. 1990; Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of adeno-type viruses.. Microbiological Reviews 54:66–74
    [Google Scholar]
  21. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  23. Schneider K., Cammack R., Schlegel H.G. 1984; Content and localization of FMN, Fe-S clusters and nickel in the NAD-linked hydrogenase of Nocardia opaca 1 b.. European Journal of Biochemistry 142:75–84
    [Google Scholar]
  24. Sensfuβ C., Reh M., Schlegel H.G. 1986; No correlation exists between the conjugative transfer of the autotrophic character and that of plasmids in Nocardia opaca strains.. Journal of General Microbiology 132:997–1007
    [Google Scholar]
  25. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  26. Stillman B. 1989; Initiation of eukaryotic DNA replication in vitro. . Annual Review of Cell Biology 5:197–245
    [Google Scholar]
  27. Vieira J., Messing J. 1987; Production of single-stranded DNA.. Methods in Enzymology 153:3–11
    [Google Scholar]
  28. Wahl G.M., Stern M., Stark G.R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl- paper and rapid hybridization by using dextran sulfate.. Proceedings of the National Academy of Sciences of the United States of America 76:3683–3687
    [Google Scholar]
  29. Waterbury P.G., Lane M.J. 1987; Generation of lambda phage concatemers for use as pulsed field electrophoresis size markers.. Nucleic Acids Research 15:3930.
    [Google Scholar]
  30. Wu X., Roy K.L. 1993; Complete nucleotide sequence of a linear plasmid from Streptomyces clavuligerus and characterization of its RNA transcripts.. Journal of Bacteriology 175:37–52
    [Google Scholar]
  31. Zaborosch C., Schneider K., Schlegel H.G., Kratzin H. 1989; Comparison of the NH2-terminal amino acid sequences of the four non-identical subunits of the NAD-linked hydrogenases from Nocardia opaca lb and Alcaligenes eutrophus HI6.. European Journal of Biochemistry 181:175–180
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-9-2055
Loading
/content/journal/micro/10.1099/00221287-139-9-2055
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error