1887

Abstract

SUMMARY: β-Glucanase synthesis in was repressed by glucose and other substrates of glycolysis. Experiments with different mutants showed that the phosphoenolpyruvate:sugar phosphotransferase system is not involved in carbon catabolite repression of β-glucanase synthesis. Carbon catabolite repression of β-glucanase synthesis was completely abolished in a mutant. An operator structure similar to those upstream of and the operon was found and was shown by site-directed mutagenesis to be the target for carbon catabolite repression of β-glucanase synthesis. The presence of this operator on a multi-copy plasmid resulted in a reduced repression of both β-glucanase and α-amylase synthesis. It seems likely that the gene encoding these enzymes are part of one regulon with respect to catabolite repression.

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1993-09-01
2021-04-22
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