1887

Abstract

SUMMARY: Mixtures of 2-aminobenzenesulphonate (trivial name orthanilic acid, OA) and 3-methylbenzoic acid (3-MB), which are degraded by enzymes of plasmid-encoded pathways, can exert inhibition of growth and respiration in sp. O-1 and mt-2 depending on the ratio of their concentrations. The pronounced inhibition of sp. O-1 growing on OA by the addition of equimolar amounts of 3-MB is characterized by a rapid inactivation of the OA-converting desulphonation activity. The exconjugant sp. O/T was selected for simultaneous breakdown of OA and 3-MB by assembling the catabolic pathways from the plasmids pSAH (OA) and pWW0 (3-MB) of the above strains. The transpositional insertion of the TOL catabolic genes (Tn) from pWW0 into the recombinant plasmid of the exconjugant O/T was detected by Southern blot hybridization using the TOL plasmid as a probe. The exconjugant showed a rapid inactivation of OA desulphonation activity similar to the parent strain. However, following induction of the TOL catabolic genes and mineralization of 3-MB, the exconjugant O/T recovered and displayed high desulphonation activity, thus allowing sequential breakdown of both substrates. Our results clearly extend the expression range of the TOL catabolic genes, but not the replication ability of the plasmid, to the genus

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1993-09-01
2024-04-23
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References

  1. Arvin E., Jensen B.K., Gundersen A.T. 1989; Substrate interactions during aerobic degradation of benzene.. Applied and Environmental Microbiology 55:33221–33225
    [Google Scholar]
  2. Assinder S.J., Williams P.A. 1990; The TOL plasmids: determinants of the catabolism of toluene and xylenes.. Advances in Microbiology and Physiology 31:1–69
    [Google Scholar]
  3. Auling G. 1993; Pseudomonads.. In Biotechnology 1, 2nd edn.. Rehm H.-J., Reed G., Reed G., Puhler A., Stadler P. Edited by Weinheim: VCH;401–431
    [Google Scholar]
  4. Auling G., Busse H.-J., Pilz F., Webb L., Kneifel H., Claus D. 1991; Rapid differentiation, by polyamine analysis, of Xanthomonas strains from phytopathogenic pseudomonads and other members of the class Proteobacteria interacting with plants.. International Journal of Systematic Bacteriology 55:223–228
    [Google Scholar]
  5. Bauer J.E., Capone D.G. 1988; Effects of co-occurring aromatic hydrocarbons on the degradation of individual polycyclic aromatic hydrocarbons in marine slurries.. Applied and Environmental Microbiology 54:1649–1655
    [Google Scholar]
  6. Benson S., Shapiro J. 1978; TOL is a broad-host range plasmid.. Journal of Bacteriology 135:278–280
    [Google Scholar]
  7. Bradford M. M. 1976; A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.. Andytircdl Biocktrmistry 135:278–280
    [Google Scholar]
  8. Burlage R.S., Hooper S.W., Sayler G.S. 1989; The TOL (pWWO) catabolic plasmid.. Applied and Environmental Microbiology 55:1323–1328
    [Google Scholar]
  9. Busse H., Auling G. 1988; Polyamine pattern as a chemo- taxonomic marker within the Proteobacteria. . Systematic and Applied Microbiology 11:1–8
    [Google Scholar]
  10. Busse H.J., Auling G. 1992; The genera Alcaligenes and “ Achromobacter’. . In The Prokaryotes. A Handbook on the Biology of Bacteria. Ecophysiology, Isolation, Identification, Application,, 2nd edn. pp. 2544–2555 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. Edited by New York: Springer-Verlag.;
    [Google Scholar]
  11. Busse H.J., El-Banna T., Auling G. 1989; Evaluation of different approaches for identification of xenobiotic-degrading pseudomonads.. Applied and Environmental Microbiology 55:1578–1583
    [Google Scholar]
  12. Hughes E.J.L., Bayly R.C., Skurray R.A. 1984; Characterization of a TOL-Iike plasmid from Alcaligenes eutrophusthat controls expression of a chromosomally encoded p-cresot pathway.. Journal of Bacteriology 158:73–78
    [Google Scholar]
  13. Jahnke M. 1990 Plasmidvermittelte Mineralisierung von aromat¬ischen Sulfonsäuren in Alcaligenes sp. 0-1 und Erweiterung der Abbauleistung durch Konjugation im terrestrischen Mikrokosmos. Ramamurthy T, Bhattacharya SK. PhD thesis University of Hannover; Germany:
    [Google Scholar]
  14. Jahnke M., El-BANNA T., Kuntworth R., Auling G. 1990; Mineralization of orthanilic acid is a plasmid-associated trait in Alcaligenes sp.O-1. Journal of General Microbiology 1362241–2249
    [Google Scholar]
  15. Johnston J.B., Murray K., Cain R.B. 1975; Microbial metabolism of aryl sulphonates. A re-assessment of colorimetric methods for the determination of sulphite and their use in measuring desulphonation of aryl and alkylbenzene sulphonates.. Antonie van Leeuwenhoek 41:493–511
    [Google Scholar]
  16. Keil H., Keil S., Pickup R.W., Williams P.A. 1985; Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWWO and pWW53.. Journal of Bacteriology 164:887–895
    [Google Scholar]
  17. Lehrbach P.R., Mcgregor I., Ward J.M., Broda P. 1983; Molecular relationships between Pseudomonas Inc P-9 degradative plasmids TOL, NAH, and SAL.. Plasmid 10:164–174
    [Google Scholar]
  18. Rojo F., Knackmuss H.-J., Timmis K.N. 1987; Assemblage of ortho cleavage route for simultaneous degradation of chloro- and methylaromatics.. Science 238:1395–1398
    [Google Scholar]
  19. Schmidt E., Hellwig M., Knackmuss H.-J. 1983; Degradation of chlorophenols by a defined mixed microbial community.. Applied and Environmental Microbiology 46:1083–1044
    [Google Scholar]
  20. Siegmund D., Diekmann H. 1989; Estimation of fermentation biomass concentration by measuring oxygen uptake off-line with an oxygen electrode.. Applied Microbiology and Biotechnology 32:32–36
    [Google Scholar]
  21. Smith M.R. 1990; The biodegradation of aromatic hydrocarbons by bacteria.. Biodegradation 1:191–206
    [Google Scholar]
  22. Thurnheer T., KÖHLER T., Cook A.M., Leisinger T. 1986; Orthanilic acid and analogues as carbon sources for bacteria: growth physiology and enzymic desulphonation.. Journal of General Micro¬biology 132:1215–1220
    [Google Scholar]
  23. Thurnheer T., ZÜRRER D., HÖGLINGER O., Leisinger T., Cook A.M. 1990; Initial steps in the degradation of benzene sulfonic acids, 4-toluene sulfonic acid, and orthanilic acid in Alcaligenes sp. strain O-l.. Biodegradation 1:55–64
    [Google Scholar]
  24. Weide H. 1983; Mikrobielle Verwertung von Mischsubstraten.. Zeitschrift fur Allgemeine Mikrobiologie 23:37–70
    [Google Scholar]
  25. Williams P.A., Murray K. 1974; Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid.. Journal of Bacteriology 120:416–423
    [Google Scholar]
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