1887

Abstract

SUMMARY: Coronatine-producing and non-producing strains of pv. have been examined. We found a connection between copper resistance and synthesis of coronatine. Published data implied that these properties may be encoded on different plasmids. Production of coronatine and copper resistance were also found to be correlated for pv. in 19 field-isolates from leaf spots of plants in a soybean field and in 28 strains of a bacterial culture collection. Genomic diversity within pv. was investigated by plasmid profiling, DNA hybridization studies and PCR analysis. All strains unable to produce coronatine (cor) were sensitive to copper ions and showed no homology to DNA from plasmid pSAY1, which carries a gene cluster for steps in coronatine production. In addition, cor strains could be distinguished from coronatine-producing strains by a single unique band when amplified by random primer PCR. Plasmid profiles of strains isolated from field-populations during 1983, 1985 and 1990 showed that coronatine-producing and non-producing strains were present. The plasmid patterns also varied in 28 strains examined from a culture collection. No correlation between plasmid patterns and race specificity was observed. Cosmid pSAY1 proved to be an effective probe for detection of the coronatine synthesis genes and also revealed polymorphisms in coronatine producing strains of pv.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-8-1927
1993-08-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/8/mic-139-8-1927.html?itemId=/content/journal/micro/10.1099/00221287-139-8-1927&mimeType=html&fmt=ahah

References

  1. Abo-Moch F., Mavridis A. 1991; Occurrence of races of Pseudomonas syringaepv. glycineain Europe and their capability to produce coronatine.. Proceedings of the 4th International Working Group on Pseudomonas syringae Pathovars pp. 227–233
    [Google Scholar]
  2. Bender C.L., Cooksey D.A. 1986; Indigenous plasmids in Pseudomonas syringaepv. tomato :conjugative transfer and role in copper resistance.. Journal of Bacteriology 165:534–541
    [Google Scholar]
  3. Bender C.L., Cooksey D.A. 1987; Molecular cloning of copper resistance genes from Pseudomonas syringaepv. tomato. . Journal of Bacteriology 169:470–474
    [Google Scholar]
  4. Bender C.L., Stone H.E., Sims J.J., Cooksey D.A. 1987; Reduced pathogen fitness of Pseudomonas syringaepv. tomatoTn5 mutants defective in coronatine production.. Physiological and Molecular Plant Pathology 30:273–283
    [Google Scholar]
  5. Bender C.L., Malvick D.K., Mitchell R.E. 1989; Plasmidmediated production of the phytotoxin coronatine in Pseudomonas syringaepv.tomato.. Journal of Bacteriology 171:807–812
    [Google Scholar]
  6. Bender C.L., Young S.A., Mitchell R.E. 1991; Conservation of plasmid DNA sequences in coronatine producing pathovars of Pseudomonas syringae. . Applied and Environmental Microbiology 57:993–999
    [Google Scholar]
  7. Bradbury J.F. 1988; Identification of cultivable bacteria from plants and plant tissue cultures by use of simple classical methods.. Acta Horticulture 225:27–37
    [Google Scholar]
  8. Cha J.S., Cooksey D.A. 1991; Copper resistance in Pseudomonas syringaemediated by periplasmic and outer membrane proteins.. Proceedings of the National Academy of Sciences of the United States of America 88:8915–8919
    [Google Scholar]
  9. Cook A.A., Stall R.E. 1982; Distribution of races of Xanthomonas campestrispathogenic on pepper.. Plant Disease 66:388–389
    [Google Scholar]
  10. Cross J.E., Kennedy B.W., Lampbert J.W., Cooper R.L. 1966; Pathogenic races of the bacterial blight pathogen of soybean, Pseudomonas glycinea. . Plant Disease Reports 50:557–560
    [Google Scholar]
  11. Denny T.P. 1988; Phenotypic diversity in Pseudomonas syringaepv. tomato. . Journal of General Microbiology 134:1939–1948
    [Google Scholar]
  12. Fett W.F., Sequeira L. 1981; Further characterization of the physiologic races of Pseudomonas glycinea. . Canadian Journal of Botany 59:283–287
    [Google Scholar]
  13. Gnanamanickam S.S., Starratt A.N., Ward E.W.B. 1982; Coronatine production in vitroand in plantaand its relation to symptom development in bacterial blight of soybean.. Canadian Journal of Botany 60:645–650
    [Google Scholar]
  14. Hugh R., Leifson R. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria.. Journal of Bacteriology 66:24–26
    [Google Scholar]
  15. Keen N.T., Staskawicbz B. 1988; Host range determinants in plant pathogens and symbionts.. Annual Review of Microbiology 42:421–440
    [Google Scholar]
  16. King E.O., Ward M.K., Raney D.E. 1954; Two simple media for the demonstration of pyocyanin and fluorescin.. Journal of Laboratory and Clinical Medicine 44:301–307
    [Google Scholar]
  17. King G.J. 1988; A DNA fingerprinting system for phytopathogenic pseudomonads.. Mededelingen van de Faculteit Landbouwwetenschap-pen Rijksuniversiteit Gent 53:1679–1684
    [Google Scholar]
  18. Leary J.V., Willis J.W., Trollinger D. 1987; Molecular genetics of coronatine production in Pseudomonas syringaepv. glycinea. . In Plant Pathogenic Bacteria pp. 498–508 Civerolo E.L., Colmer D., Davis R.E., Gillaspie A.G. Edited by Dordrecht: Martinus Nijhof Publications.;
    [Google Scholar]
  19. Lelliott R.A., Billing E., Hayward A.C. 1966; A determinative scheme for the fluorescent plant pathogenic Pseudomonads.. Journal of Applied Bacteriology 29:470–480
    [Google Scholar]
  20. Ma S.W., Morris V.L., Cuppels D.A. 1991; Characterization of a DNA region required for production of the phytotoxin coronatine by Pseudomonas syringaepv tomato. . Molecular Plant- Microbe Interactions 4:69–74
    [Google Scholar]
  21. Maniate T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  22. Martin G.B., Williams J.G.K., Tanksley S.D. 1991; Rapid identification of markers linked to a Pseudomonasresistance gene in tomato by using random primers and near-isogenic lines.. Proceedings of the National Academy of Sciences of the United States of America 88:2336–2340
    [Google Scholar]
  23. Mellano A.M., Cooksey D.A. 1988; Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringaepv. tomato. . Journal of Bacteriology 170:2879–2883
    [Google Scholar]
  24. Mitchell R.E. 1982; Coronatine production by some phytopathogenic pseudomonads.. Physiological Plant Pathology 20:83–89
    [Google Scholar]
  25. Mitchell R.E. 1985; Coronatine biosynthesis: incorporation of L-[U-14C]isoleucine and L-[U-14C]threonine into the 1-amino-l- carboxy-2-ethylcyclopropyl moiety.. Phytochemistry 24:247–249
    [Google Scholar]
  26. Mitchell R.E. 1991; Coronatine analogs produced by Xanthomonas campestrispv. phormicola. . Phytochemistry 30:3917–3920
    [Google Scholar]
  27. Nishiyama K., Sakai R., Ezuka A., Ichihara A., Shiraishi K., Ogasawara M., Sato H., Sakamura S. 1976; Phytotoxic effect of coronatine produced by Pseudomonas coronafaciensvar. atropurpreaon leaves of Italian ryegrass.. Annals of the Phytopathological Society of Japan 42:613–614
    [Google Scholar]
  28. Parry R.J., Mafoti R. 1986; Biosynthesis of coronatine, a novel polyketide.. Journal of the American Chemical Society 108:4681–4682
    [Google Scholar]
  29. Parry R.J., Lin M.T., Walker A.E., Mhaskar S. 1991; The biosynthesis of coronatine: investigations of the biosynthesis of coronamic acid.. Journal of the American Chemical Society 113:1849–1850
    [Google Scholar]
  30. Sakai R., Nishiyama K., Ichihara A., Shiraishi K., Sakamura S. 1979; Studies on the mechanism of physiological activity of coronatine. Effect of coronatine on cell wall extensibility and expansion of potato tuber tissue.. Annals of the Phytopathological Society of Japan 45:645–653
    [Google Scholar]
  31. Sato M., Nishiyama K., Shirata A. 1983; Involvement of plasmid DNA in the productivity of coronatine by Pseudomonas syringaepv.atropurpurea.. Annals of the Phytopathological Society of Japan 49:522–528
    [Google Scholar]
  32. SchÄfer C., WÖstemeyer J. 1992; Random primer dependent PCR differentiates aggressive from non-aggressive isolates of the oilseed rape Phoma lingam( Leptosphaeria maculans). . Journal of Phytopathology 136:124–136
    [Google Scholar]
  33. Stall R.E., Loschke D.C., Jones J.B. 1986; Linkage of copper resistance and avirulence loci on a self-transmissable plasmid in Xanthomonas campestris pv. vesicatoria. . Phytopathology 76:240–243
    [Google Scholar]
  34. Staskawicz B.J., Panopoulos N.J. 1979; A rapid and sensitive microbiological assay for phaseolotoxin.. Phytopathology 69:663–666
    [Google Scholar]
  35. Ullrich M., Geider K., Fritsche W. 1991; Plasmid patterns of Pseudomonas syringaepv. glycineastrains with or without coronatine production.. Proceedings of the 4th International Working Group of Phytopathogenic Pseudomonads pp. 324–329
    [Google Scholar]
  36. Volksch B., Bublitz F., Fritsche W. 1989; Coronatine production by Pseudomonas syringaepathovars: screening method and capacity of production formation.. Journal of Basic Microbiology 29:463–468
    [Google Scholar]
  37. Welsh J., Mcclelland M. 1990; Fingerprinting genomes using PCR with arbitrary primers.. Nucleic Acids Research 18:7213–7218
    [Google Scholar]
  38. Welsh J., Petersen C., Mcclelland M. 1991; Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping.. Nucleic Acids Research 19:303–306
    [Google Scholar]
  39. Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A., Tingey S.V. 1990; DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.. Nucleic Acids Research 18:6531–6535
    [Google Scholar]
  40. Young S.A. 1991 Isolation of genes involved in coronatine biosynthesis from P. syringae pv. tomato and P. syringae pv. glycinea PG4180. M.S. thesis Oklahoma State University; USA.:
    [Google Scholar]
  41. Young S.A., Park S.K., RODGERS C., Mitchell R.E., Bender C.L. 1992; Physical and functional characterization of the gene cluster encoding the polyketide phytotoxin coronatine in Pseudomonas syringaepv. glycinea. . Journal of Bacteriology 174:1837–1843
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-8-1927
Loading
/content/journal/micro/10.1099/00221287-139-8-1927
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error