1887

Abstract

SUMMARY: -AcetyI-D-[1-C]glucosamine ([C]GlcNAc) was used to label specifically the cell wall in Lysozyme solubilized 86.5% of the radioactivity present in the trichloroacetic acid-precipitated and RNAase plus trypsin-digested fraction of hyphae pulse-labelled with [C]GlcNAc for 3 min in a medium containing glucose, yeast extract and a mixture of nine amino acids. Experiments with [C]GlcNAc-labelled hyphae revealed that growth of occurs without turnover of peptidoglycan. Autoradiographic analysis of hyphae indicated that cell wall elongation occurs by intercalation of newly synthesized wall polymers at the tip, but also over a relatively broad zone that, depending on the hyphal length, extends up to 6-10 μm from the tip.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-8-1915
1993-08-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/8/mic-139-8-1915.html?itemId=/content/journal/micro/10.1099/00221287-139-8-1915&mimeType=html&fmt=ahah

References

  1. Boothby D., Daneo-Moore L., Higgins M.L., Coyette J., Shockman G.D. 1973; Turnover of bacterial cell wall pepti- doglycans.. Journal of Biological Chemistry 248:2161–2169
    [Google Scholar]
  2. BraÑa A.F., Manzanal M.B., Hardisson C. 1982; Mode of cell wall growth of Streptomyces antibioticus. . FEMS Microbiology Letters 13:231–235
    [Google Scholar]
  3. Caro L.G., Van Tubergen R.P. 1962; High resolution autoradiography.. Journal of Cell Biology 15:173–188
    [Google Scholar]
  4. Doyle R.J., Chaloupka J., Vinter V. 1988; Turnover of cell walls in microorganisms.. Microbiological Reviews 52:554–567
    [Google Scholar]
  5. Frehel C., Ryter A. 1979; Peptidoglycan turnover during growth of a Bacillus megaterium DAP LYS mutant.. Journal of Bacteriology 137:947–955
    [Google Scholar]
  6. Gray D.I., Gooday G.W., Prosser J.I. 1990; Apical hyphal extension in Streptomyces coelicolor A3(2).. Journal of General Microbiology 136:1077–1084
    [Google Scholar]
  7. Hardisson C., Manzanal M.B., Salas J.A., Suarez J.E. 1978; Fine structure and biochemistry of arthrospore germination in Streptomyces antibioticus. . Journal of General Microbiology 105:203–214
    [Google Scholar]
  8. Kretschmer S. 1982; Dependence of the mycelial growth pattern on the individually regulated cell cycle in Streptomyces granaticolor. . Zeitschrift für allgemeine Mikrobiologie 22:335–347
    [Google Scholar]
  9. Mobley H.L.T., Doyle R.J., Streips U.N., Langemeier S.O. 1982; Transport and incorporation of N-acetyl-d-glucosamine in Bacillus subtilis. . Journal of Bacteriology 150:8–15
    [Google Scholar]
  10. Mobley H.L.T., Koch A.L., Doyle R.J., Streips U.N. 1984; Insertion and fate of the cell wall in Bacillus subtilis. . Journal of Bacteriology 158:169–179
    [Google Scholar]
  11. Naumova I.B., Kuznetsov V.D., Kudrina K.S., Bezzubenkova A.P. 1980; The occurrence of teichoic acids in streptomycetes.. Archives of Microbiology 126:71–75
    [Google Scholar]
  12. Pooley H.M. 1976; Turnover and spreading of old wall during surface growth of Bacillus subtilis. . Journal of Bacteriology 125:1127–1138
    [Google Scholar]
  13. Pooley H.M., Karamata D. 1984; Flagellation and the control of autolysin activity in Bacillus subtilis. . In Microbial Cell Wall Synthesis and Autolysis pp. 13–19 Edited by Nombela C. Amsterdam: Elsevier Science Publishers:
    [Google Scholar]
  14. Schuhmann E., Bergter F. 1976; Mikroskopische untersuchungen zur wachstumskinetik von Streptomyces hygro- scopicus. . Zeitschrift für allgemeine Mikrobiologie 16:201–215
    [Google Scholar]
  15. Shirling G.G., Gottlieb D. 1966; Methods for characterization of Streptomyces species.. International Journal of Systematic Bacteriology 16:313–340
    [Google Scholar]
  16. Trentini W.C., Murray R.G.E. 1975; Ultrastructural effects of lysozyme on the cell wall of Caryophanon latum. . Canadian Journal of Microbiology 21:164–172
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-8-1915
Loading
/content/journal/micro/10.1099/00221287-139-8-1915
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error