%0 Journal Article %A Buzzi, Marcelo %A Felipe, Maria Sueli Soares %A de Oliveira Azevedo, Maristella %A de Araujo Caldas, Ruy %T Membrane lipid composition and invertase secretion of Neurospova crassa and its wall-less mutant slime: effects of temperature and the surfactant Tween 80 %D 1993 %J Microbiology, %V 139 %N 8 %P 1885-1889 %@ 1465-2080 %R https://doi.org/10.1099/00221287-139-8-1885 %I Microbiology Society, %X SUMMARY: The effects of temperature and the surfactant Tween 80 on the secretion of invertase by the ascomycete fungus Newospora crassa and its wall-less strain slime were investigated. Temperature acclimation dramatically affects the phospholipid fatty acid pattern in both strains. The levels of polyunsaturated fatty acids in membrane lipids of wild-type Neurospova crassa and slime increased as growth temperature decreased. Chromatogram analysis from cultures acclimated to 15 C showed high levels of linolenic acid (18:3), and low levels of oleic acid (18:1), suggesting desaturation. Reducing the temperature during growth to 15 C affected phospholipid fatty acid composition in both strains, which resulted in a higher level of invertase secretion. The wild-type Newospora crassa showed no difference in invertase secretion in the presence of Tween 80. However, the addition of the surfactant to slime cultures caused a 60 % increase in invertase secretion, which was more evident after 48 h incubation. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-8-1885