1887

Abstract

SUMMARY: The effects of temperature and the surfactant Tween 80 on the secretion of invertase by the ascomycete fungus and its wall-less strain slime were investigated. Temperature acclimation dramatically affects the phospholipid fatty acid pattern in both strains. The levels of polyunsaturated fatty acids in membrane lipids of wild-type and slime increased as growth temperature decreased. Chromatogram analysis from cultures acclimated to 15 C showed high levels of linolenic acid (18:3), and low levels of oleic acid (18:1), suggesting desaturation. Reducing the temperature during growth to 15 C affected phospholipid fatty acid composition in both strains, which resulted in a higher level of invertase secretion. The wild-type showed no difference in invertase secretion in the presence of Tween 80. However, the addition of the surfactant to slime cultures caused a 60 % increase in invertase secretion, which was more evident after 48 h incubation.

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/content/journal/micro/10.1099/00221287-139-8-1885
1993-08-01
2021-05-14
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References

  1. Arnold W.N. 1974; Expression of cryptic fructofuranosidase in Saccharomyces rouxii.. Journal of Bacteriology 120:886–894
    [Google Scholar]
  2. Bell M.V., Henderson R.J., Sargent J.R. 1986; The role of polyunsaturated fatty acids in fish.. Comparative Biochemistry and Physiology 83B:711–719
    [Google Scholar]
  3. Bigger C.H., White M.R., Braymer H.D. 1972; Ultrastructure and invertase secretion of the slime mutant of Neurospora crassa.. Journal of General Microbiology 71:159–166
    [Google Scholar]
  4. Christie W.W. 1982; The isolation of lipids from tissues.. In Lipid Analysis, 2nd edn. pp. 17–23 Oxford:: Pergamon Press.;
    [Google Scholar]
  5. Folch J., Lees M., Stanley G.H. 1957; A simple method for the isolation and purification of total lipids from animal tissues.. Journal of Biological Chemistry 226:497–509
    [Google Scholar]
  6. Fukushima H., Martin C.E., Iida H., Kitajima Y., Thompson G.A. JR Nozawa Y. 1976; Changes in membrane lipid composition during temperature adaptation by a thermotolerant strain of Tetrahymena pyriformis.. Biochimica et Biophysica Acta 431:165–179
    [Google Scholar]
  7. Gratzner H.G., Sheenan D.N. 1969; Neurospora mutant exhibiting the hyperproduction of amylase and invertase.. Journal of Bacteriology 97:544–549
    [Google Scholar]
  8. Hunter K., Rose A.H. 1972; Lipid composition of Saccharomyces cerevisiae as influenced by growth temperature.. Biochimica et Biophysica Acta 260:639–653
    [Google Scholar]
  9. Lampen J.O. 1971; Yeast and Neurospora invertase.. In The Enzymes, 3rd edn. V pp. 291–305, Edited by. Boyer P.D. New York:: Academic Press.;
    [Google Scholar]
  10. Martin C.E., Siegel D., Aaronson L.R. 1981; Effects of temperature acclimation on Neurospora phospholipids.. Biochimica et Biophysica Acta 665:399–407
    [Google Scholar]
  11. Pietro R.C.L.R., Jorge J.A., Terenzi H.F. 1989; Pleiotropic deficiency in the control of carbon-regulated catabolic enzymes in the slime variant of Neurospora crassa.. Journal of General Microbiology 135:1375–1382
    [Google Scholar]
  12. Reese E.T., Maguire A. 1969; Surfactants as stimulants of enzyme production by microorganisms.. Applied Microbiology 17:242–245
    [Google Scholar]
  13. Skriver L., Thompson G.A.JR. 1979; Temperature-induced changes in fatty acid unsaturation of Tetrahymena membranes do not require induced fatty acid desaturase synthesis.. Biochimica et Biophysica Acta 572:376–381
    [Google Scholar]
  14. Vogel H.J. 1964; Distribution of lysine pathways among fungi: evolutionary implications.. American Naturalist 98:435–446
    [Google Scholar]
  15. Yazdi M.T., Woodward J.R., Radford A. 1990; The cellulase complex of Neurospora crassa : activity, stability and release.. Journal of General Microbiology 136:1313–1319
    [Google Scholar]
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