Characterization of an IS-like element from Free

Abstract

SUMMARY: A DNA sequence, present in members of the complex, has been identified and characterized. The distribution of this DNA sequence among mycobacterial species was analysed by DNA hybridization and PCR experiments. As the sequence was detected only in bacteria belonging to the complex, it may be useful for the rapid discrimination of mycobacteria. Interestingly, the sequence has some characteristics of an insertion element (IS) and codes for a hypothetical protein with significant homologies to proteins encoded by several IS elements of other organisms, namely IS and IS from IS from Tn from and IS from Together, these elements form a previously unrecognized family of transposable elements. This finding suggests the possibility of horizontal gene transfer between pathogenic mycobacteria and other organisms including Gram-negative plant-pathogenic bacteria.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-8-1767
1993-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/8/mic-139-8-1767.html?itemId=/content/journal/micro/10.1099/00221287-139-8-1767&mimeType=html&fmt=ahah

References

  1. Cave M.D., Eisenach K.D., Mcdernott P.F., Bates J.H., Crawford J.T. 1991; IS6110:conservation of sequences in theMycobacterium tuberculosiscomplex and its utilization in DNA fingerprinting.. Molecular and Cellular Probes 5:73–80
    [Google Scholar]
  2. Chen C.W., Yu T.-W., Chung H.-M., Chou C.-F. 1992; Discovery and characterization of a new transposable element, Tn4811, Streptomyces lividans66.. Journal of Bacteriology 174:7762–7769
    [Google Scholar]
  3. Cirillo J.F., Barletta R.G., Bloom B.R., Jacobs W.R. JR 1991; A novel transposon trap for mycobacteria: isolation and characterization of IS1096. . Journal of Bacteriology 173:7772–7780
    [Google Scholar]
  4. Clark-Curtiss J.E., Docherty M.A. 1989; A species-specific repetitive sequence in Mycobacterium leprae DNA.. Journal of Infectious Diseases 159:7–15
    [Google Scholar]
  5. De Meirsman C., Van Soom C., Verreth C., Van Gool A., Vanderleyden J. 1990; Nucleotide sequence analysis of IS427and its target sites inAgrobacterium tumefaciensT37.. Plasmid 24:227–234
    [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Research 12:387–395
    [Google Scholar]
  7. Eisenach K.D., Cave M.D., Bates J.H., Crawford J.T. 1990; Polymerase Chain reaction amplification of a repetitive DNA sequence specific forMycobacterium tuberculosis. . Journal of Infectious Diseases 161:977–981
    [Google Scholar]
  8. Van Embden J.D.A., Cave M.D., Crawford J.T., Dale J.W., Eisenach K.D., Gicquel B., Hermans P., Martin C., Mcadam R., Shinnick T.M., Small P.M. 1993; Strain identification ofMycobacterium tuberculosisby DNA fingerprinting: Recommendations for a standardized methology.. Journal of Clinical Microbiology 31:406–409
    [Google Scholar]
  9. Ferrante A.A., Lessie R.G. 1991; Nucleotide sequence ofIS402fromPseudomonas cepacia. . Gene 102:143–144
    [Google Scholar]
  10. Gormley E.P., Davies J. 1991; Transfer of plasmid RSF1010 by conjugation fromEscherichia colitoStreptomyces lividansandMycobacterium smegmatis. . Journal of Bacteriology 173:6705–6708
    [Google Scholar]
  11. Green E.P., Tizard M.L., Thompson J., Winterbourne J.D., Mcfadden J.J., Hermon-Taylor J. 1989; Sequence and characteristics of IS900, an insertion element identified in a human Crohn̓s disease isolate ofMycobacterium paratuberculosis. . Nucleic Acids Research 17:9063–9073
    [Google Scholar]
  12. Grosskinsky C.M., Jacobs W.R. JR Clark-Curtiss J.E., Bloom B.R. 1989; Genetic relationships amongMycobacterium leprae,Mycobacterium tuberculosis,and candidate leprosy vaccine strains determined by DNA hybridization: identification of anM. leprae-specific repetitive sequence.. Infection and Immunity 57:1535–1541
    [Google Scholar]
  13. Hoover T.A., Vodkin M.H., Williams J.C. 1992; ACoxiella burnetiirepeated DNA element resembling a bacterial insertion sequence.. Journal of Bacteriology 174:5540–5548
    [Google Scholar]
  14. Iida S., Meyer J., Arber W. 1983; Prokaryotic IS elements.. In Mobile Genetic Elements, pp. 159–221 Shapiro J.A. Edited by New York: Academic Press;
    [Google Scholar]
  15. Kuehn M., Lent K., Haas J., Hagenzieker J., Cervin M., Smith A.L. 1992; Fimbriation ofPseudomonas cepacia. . Infection and Immunity 60:2002–2007
    [Google Scholar]
  16. Kunze Z.M., Wall S., Appelberg R., Silva M.T., Portaels F., Mcfadden J.J. 1991; IS901, a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity inMycobacterium avium. . Molecular Microbiology 5:2265–2272
    [Google Scholar]
  17. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  18. Martin C., Timm J., Rauzier J., Gomez-Lus R., Davies J., Gicquel B. 1990; Transposition of an antibiotic resistance element in mycobacteria.. Nature; London: 345739–743
    [Google Scholar]
  19. Mcadam R.A., Hermans P.W.M., Van Soolingen D., Zainuddin Z.F., Catty D., Van Embden J.D.A., Dale J.W. 1990; Characterization of aMycobacterium tuberculosisinsertion sequence belonging to the IS3 family.. Molecular Microbiology 4:1607–1613
    [Google Scholar]
  20. Mcfadden J.J., Butcher P.D., Chiodini R., Hermon-Taylor J. 1987; Crohn̓s disease-isolated mycobacteria are identical toMycobacterium paratuberculosis,as determined by DNA probes that distinguish between mycobacterial species.. Journal of Clinical Microbiology 25:796–801
    [Google Scholar]
  21. Moss M.T., Malik Z.P., Tizard M.L., Green E.P., Sanderson E.D., Hermon-Taylor J. 1992; IS902, an insertion element of the chronic-enteritis-causingMycobacterium aviumsubsp.silvaticum.. Journal of General Microbiology 138:139–145
    [Google Scholar]
  22. Patel R.J., Fries J.W.U., Piessens W.F., Wirth D.F. 1990; Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification ofMycobacterium tuberculosis. . Journal of Clinical Microbiology 28:513–518
    [Google Scholar]
  23. Paulus I., Canady J., Vincent F., Bonnard G., Kares C., Otten L. 1991; Sequence of theiaaandiptregion of differentAgrobacterium tumefaciensbiotype III octopine strains: reconstruction of octopine Ti plasmid evolution.. Plant Molecular Biology 16:601–614
    [Google Scholar]
  24. Rappuoli R., Gross R. 1990; attsites,toxgene and insertion elements as tools for the diagnosis and molecular epidemiology ofCorynebacterium diphtheriae. . In Gene Probes for Bacteria pp. 205–232 Macario A.J.L., Conway de Macario E. Edited by New York: Academic Press;
    [Google Scholar]
  25. Runyon E.H. 1959; Anonymous mycobacteria in pulmonary disease.. Medical Journal of Northern America 43:273–290
    [Google Scholar]
  26. Scordilis G.E., Ree H., Lessie T.G. 1987; Identification of transposable elements which activate gene expression inPseudomonas cepacia. . Journal of Bacteriology 169:8–13
    [Google Scholar]
  27. Soto M.J., Zarzano A., Olivares J., Toro N. 1992; Unpublished sequence. GenEMBL accession number X65471..
    [Google Scholar]
  28. Thierry D., Brisson-Noel A., Levy-Frebault V., Nguyen S., Guesdon J., Gicquel B. 1990; Characterization of aMycobacterium tuberculosisinsertion sequence, IS6110and its application in diagnosis.. Journal of Clinical Microbiology 28:2668–2673
    [Google Scholar]
  29. Vanderleyden J., Desair J., De Meirsman C., Michiels K., Van Gool A.P., Chilton M.D., Jen G.C. 1986; Nucleotide sequence of an insertion sequence element of the Ti-plasmid Ti37.. Nucleic Acids Research 14:6699–6709
    [Google Scholar]
  30. Vismara D., Filippone Mezzopreti M., Gilardini M., Del Porto P., Lombardi G., Piccolella E., Damiani G., Rappuoli R., Colizzi V. 1990; Identification of a 35-KilodaltonMycobacterium tuberculosisprotein containing B- and T-cell epitopes.. Infection and Immunity 58:245–251
    [Google Scholar]
  31. Woods S.A., Cole S.T. 1990; A family of dispersed repeats inMycobacterium leprae. . Molecular Microbiology 4:1745–1751
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-8-1767
Loading
/content/journal/micro/10.1099/00221287-139-8-1767
Loading

Data & Media loading...

Most cited Most Cited RSS feed