SUMMARY: A DNA sequence, present in members of the Mycobacterium tuberculosis complex, has been identified and characterized. The distribution of this DNA sequence among mycobacterial species was analysed by DNA hybridization and PCR experiments. As the sequence was detected only in bacteria belonging to the M. tuberculosis complex, it may be useful for the rapid discrimination of mycobacteria. Interestingly, the sequence has some characteristics of an insertion element (IS) and codes for a hypothetical protein with significant homologies to proteins encoded by several IS elements of other organisms, namely IS427 and IS869 from Agrobacterium tumefaciens, IS402 from Pseudomonas cepacia, Tn4811 from Streptomyces lividans and ISRm4 from Rhizobium meliloti. Together, these elements form a previously unrecognized family of transposable elements. This finding suggests the possibility of horizontal gene transfer between pathogenic mycobacteria and other organisms including Gram-negative plant-pathogenic bacteria.
CaveM.D.,
EisenachK.D.,
McdernottP.F.,
BatesJ.H.,
CrawfordJ.T.1991; IS6110:conservation of sequences in theMycobacterium tuberculosiscomplex and its utilization in DNA fingerprinting.. Molecular and Cellular Probes 5:73–80
ChenC.W.,
YuT.-W.,
ChungH.-M.,
ChouC.-F.1992; Discovery and characterization of a new transposable element, Tn4811, Streptomyces lividans66.. Journal of Bacteriology 174:7762–7769
CirilloJ.F.,
BarlettaR.G.,
BloomB.R.,
JacobsW.R.JR1991; A novel transposon trap for mycobacteria: isolation and characterization of IS1096.
. Journal of Bacteriology 173:7772–7780
De MeirsmanC.,
Van SoomC.,
VerrethC.,
Van GoolA.,
VanderleydenJ.1990; Nucleotide sequence analysis of IS427and its target sites inAgrobacterium tumefaciensT37.. Plasmid 24:227–234
EisenachK.D.,
CaveM.D.,
BatesJ.H.,
CrawfordJ.T.1990; Polymerase Chain reaction amplification of a repetitive DNA sequence specific forMycobacterium tuberculosis.
. Journal of Infectious Diseases 161:977–981
GormleyE.P.,
DaviesJ.1991; Transfer of plasmid RSF1010 by conjugation fromEscherichia colitoStreptomyces lividansandMycobacterium smegmatis.
. Journal of Bacteriology 173:6705–6708
GreenE.P.,
TizardM.L.,
ThompsonJ.,
WinterbourneJ.D.,
McfaddenJ.J.,
Hermon-TaylorJ.1989; Sequence and characteristics of IS900, an insertion element identified in a human Crohn̓s disease isolate ofMycobacterium paratuberculosis.
. Nucleic Acids Research 17:9063–9073
HooverT.A.,
VodkinM.H.,
WilliamsJ.C.1992; ACoxiella burnetiirepeated DNA element resembling a bacterial insertion sequence.. Journal of Bacteriology 174:5540–5548
KunzeZ.M.,
WallS.,
AppelbergR.,
SilvaM.T.,
PortaelsF.,
McfaddenJ.J.1991; IS901, a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity inMycobacterium avium.
. Molecular Microbiology 5:2265–2272
MartinC.,
TimmJ.,
RauzierJ.,
Gomez-LusR.,
DaviesJ.,
GicquelB.1990; Transposition of an antibiotic resistance element in mycobacteria.. Nature; London: 345739–743
McadamR.A.,
HermansP.W.M.,
Van SoolingenD.,
ZainuddinZ.F.,
CattyD.,
Van EmbdenJ.D.A.,
DaleJ.W.1990; Characterization of aMycobacterium tuberculosisinsertion sequence belonging to the IS3 family.. Molecular Microbiology 4:1607–1613
McfaddenJ.J.,
ButcherP.D.,
ChiodiniR.,
Hermon-TaylorJ.1987; Crohn̓s disease-isolated mycobacteria are identical toMycobacterium paratuberculosis,as determined by DNA probes that distinguish between mycobacterial species.. Journal of Clinical Microbiology 25:796–801
MossM.T.,
MalikZ.P.,
TizardM.L.,
GreenE.P.,
SandersonE.D.,
Hermon-TaylorJ.1992; IS902, an insertion element of the chronic-enteritis-causingMycobacterium aviumsubsp.silvaticum.. Journal of General Microbiology 138:139–145
PatelR.J.,
FriesJ.W.U.,
PiessensW.F.,
WirthD.F.1990; Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification ofMycobacterium tuberculosis.
. Journal of Clinical Microbiology 28:513–518
PaulusI.,
CanadyJ.,
VincentF.,
BonnardG.,
KaresC.,
OttenL.1991; Sequence of theiaaandiptregion of differentAgrobacterium tumefaciensbiotype III octopine strains: reconstruction of octopine Ti plasmid evolution.. Plant Molecular Biology 16:601–614
RappuoliR.,
GrossR.1990; attsites,toxgene and insertion elements as tools for the diagnosis and molecular epidemiology ofCorynebacterium diphtheriae.
. In Gene Probes for Bacteria pp. 205–232 MacarioA.J.L.,
Conway de MacarioE.
Edited by New York: Academic Press;
ScordilisG.E.,
ReeH.,
LessieT.G.1987; Identification of transposable elements which activate gene expression inPseudomonas cepacia.
. Journal of Bacteriology 169:8–13
VanderleydenJ.,
DesairJ.,
De MeirsmanC.,
MichielsK.,
Van GoolA.P.,
ChiltonM.D.,
JenG.C.1986; Nucleotide sequence of an insertion sequence element of the Ti-plasmid Ti37.. Nucleic Acids Research 14:6699–6709
VismaraD.,
Filippone MezzopretiM.,
GilardiniM.,
Del PortoP.,
LombardiG.,
PiccolellaE.,
DamianiG.,
RappuoliR.,
ColizziV.1990; Identification of a 35-KilodaltonMycobacterium tuberculosisprotein containing B- and T-cell epitopes.. Infection and Immunity 58:245–251