The DNA sequence and minimal replicon of the plasmid pSR1: evidence of a common ancestry with plasmids from Free

Abstract

SUMMARY: The complete nucleotide sequence of pSR1, a 3 kb multicopy cryptic plasmid from ATCC 19223 has been determined. pSR1 is unrelated to the 4.4 kb plasmid pBL1 and shows no DNA sequence conservation with plasmids from Transposon insertion and deletion mutants located the minimal replicon to within a 2.1 kb - restriction fragment. This region contains a single large open reading frame, ORF2, flanked at the 5 end by a series of inverted repeat sequences which may modulate its expression, and at the 3’ end by a region which may contain a replication origin. ORF2 (position 1633-2636) with a maximum coding potential of 36 kDa is essential for pSR1 replication and was designated the gene. The predicted ORF2 protein product exhibits 47% identity over a length of 343 amino acids with a replication-associated ORF in the plasmid pNG2, many of the changes being in the third base position. This observation suggests that pSR1 and pNG2, which are two plasmids from environmentally separated species, may share a common ancestral gene.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-8-1753
1993-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/8/mic-139-8-1861.html?itemId=/content/journal/micro/10.1099/00221287-139-8-1753&mimeType=html&fmt=ahah

References

  1. Barker H.A., Kamen M.D. 1945; Carbon dioxide utilization in the synthesis of acetic acid by Clostridium thermoaceticum. . Proceedings of the National Academy of Sciences of the United States of America 47:301–305
    [Google Scholar]
  2. Bergmeyer H.U., Bernt E. 1974; Fructose-1,6-diphosphate aldolase.. In Methods of Enzymatic Analysis 2 pp. 1100–1105. Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  3. Bergmeyer H.U., Gawehn K., Grassl M. 1974; 3-Phospho- glycerate kinase.. In Methods of Enzymatic Analysis 1 pp. 502–503 Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  4. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Braun M., Gottschalk G. 1981; Effect of molecular hydrogen and carbon dioxide on chemo-organotrophic growth of Aceto- bacterium woodii and Clostridium aceticum. . Archives of Microbiology 128:294–298
    [Google Scholar]
  6. Dolfing J. 1988; Acetogenesis.. In Biology of Anaerobic Micro�organisms pp. 417–468 Zehnder A.J.B. Edited by New York: John Wiley.;
    [Google Scholar]
  7. Eden G., Fuchs G. 1983; Autotrophic CO2 fixation in Aceto- bacterium woodii. II. Demonstration of enzymes involved.. Archives of Microbiology 135:68–73
    [Google Scholar]
  8. Erickson L.E. 1980; Biomass elemental composition and energy content.. Biotechnology and Bioengineering 22:451–453
    [Google Scholar]
  9. Eyzaguirre J., Jansen K., Fuchs G. 1982; Phosphoenolpyruvate synthetase in Methanobacterium thermoautotrophicum. . Archives of Microbiology 132:67–74
    [Google Scholar]
  10. Godley A.R., Linnett P.E., Robinson J.P. 1990; The effect of carbon dioxide on the growth kinetics of fructose limited chemostat cultures of Acetobacterium woodii DSM 1030.. Archives of Microbiology 154:5–11
    [Google Scholar]
  11. Gruber W., Mollering H., Bergmeyer H.U. 1974; Analytical differentiation of purine and pyrimidine nucleotides. Determination of ADP, ATP and sum of GTP+ITP in biological material.. In Methods of Enzymatic Analysis 4 pp. 2078–2087 Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  12. Hess B., Wieker H.-J. 1974; Pyruvate kinase from yeast.. In Methods of Enzymatic Analysis 2 pp. 778–780 Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  13. Ingraham J.L., Maaloe O., Neidhart F.C. 1983 Growth of the Bacterial Cell. Sunderland, Mass., USA: Sinauer Associates Inc.;
    [Google Scholar]
  14. Jaworeck D., Gruber W., Bergmeyer H.U. 1974a; Adenosine- 5’-triphosphate. Determination with 3-phosphoglycerate kinase.. In Methods of Enzymatic Analysis 4 pp. 2097–2101 Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  15. Jaworeck D., Gruber W., Bergmeyer H.U. 1974b; Adenosine- 5’-diphosphate and adenosine-5’-monophosphate.. In Methods of Enzymatic Analysis 4 pp. 2102–2110 Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  16. Klingenberg M. 1974; Nicotinamide-adenine dinucleotides (NAD, NADP, NADH, NADPH). Spectrophotometric and fluorimetric methods.. In Methods of Enzymatic Analysis 4 pp. 2045–2059 Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  17. Lang G. 1974; D-Glucose-6-phosphate and D- fructose-6-phosphate.. In Methods of Enzymatic Analysis 3 pp. 1238–1242 Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  18. Lindley N.D., Gros E., Lebloas P., Cocaign M., Loubière P. 1990; Carbon and energy flow during acidogenic metabolism of unicarbon and multicarbon substrates.. In Microbiology and Bio�chemistry of Strict Anaerobes Involved in Interspecies Hydrogen Transfer pp. 213–223 Belaich J.P., Bruschi M., Garcia J.L. Edited by New York: Plenum Press.;
    [Google Scholar]
  19. Ljungdahl L.G., Andreesen J.R. 1975; Tungsten, a component of active formate dehydrogenase from Clostridium thermoaceticum. . FEBS Letters 54:279–282
    [Google Scholar]
  20. Loubière P., Lindley N.D. 1991; The use of acetate as an additional co-substrate improves methylotrophic growth of the acetogenic anaerobe Eubacterium limosum when CO2 fixation is rate- limiting.. Journal of General Microbiology 137:2247–2251
    [Google Scholar]
  21. Loubière P., Gros E., Paquet V., Lindley N.D. 1992; Kinetics and physiological implications of the growth behaviour of Eubacterium limosum on glucose/methanol mixtures.. Journal of General Microbiology 138:979–985
    [Google Scholar]
  22. Lynd L.H., Zeikus J.G. 1983; Metabolism of H2-CO2, methanol and glucose by Butyribacterium methylotrophicum. . Journal of Bacteriology 153:1415–1423
    [Google Scholar]
  23. Martin D.R., Lundie L.L., Kellum R., Drake H.L. 1983; Carbon monoxide dependent evolution of hydrogen by the homoacetate fermenting bacterium Clostridium thermoaceticum. . Current Microbiology 8:337–340
    [Google Scholar]
  24. Michal G., Beutler H.D. 1974; D-Fructose-1,6-diphosphate, dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate.. In Methods of Enzymatic Analysis 3 pp. 1314–1319 Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  25. Pacaud S., Loubière P., Goma G. 1985; Methanol metabolism by Eubacterium limosum B2: effects of pH and carbon dioxide on growth and organic acid production.. Current Microbiology 12:245–250
    [Google Scholar]
  26. Pacaud S., Loubière P., Goma G., Lindley N.D. 1986; Organic acid production during methylotrophic growth of Eubacterium limosum B2: displacement towards increased butyric acid yields by supplement with acetate.. Applied Microbiology and Biotechnology 23:330–335
    [Google Scholar]
  27. Raeburn S., Rabinowitz J.C. 1971; Pyruvate :ferredoxin oxido- reductase. II. Characteristics of the forward and the reverse reactions and properties of the enzyme.. Archives of Biochemistry and Biophysics 146:21–33
    [Google Scholar]
  28. Sharak-Genthner B.R., Davis C.L., Bryant M.P. 1981; Features of rumen and sewage sludge strains of Eubacterium limosuma methanol and H2-CO2 utilizing species.. Applied and Environmental Microbiology 42:12–19
    [Google Scholar]
  29. Vogels G.D., Visser C.M. 1983; Interconnection of methano- genic and acetogenic pathways.. FEMS Microbiology Letters 20:291–297
    [Google Scholar]
  30. Zeikus J.G., Fuchs G., Kenealy W., Thauer R.K. 1977; Oxidoreductases involved in cell carbon synthesis of Methanobacterium thermoautotrophicum. . Journal of Bacteriology 132:604–613
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-8-1753
Loading
/content/journal/micro/10.1099/00221287-139-8-1753
Loading

Data & Media loading...

Most cited Most Cited RSS feed