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Volume 139, Issue 8

Characterization of by 16S DNA restriction fragment length polymorphisms Free

Abstract

SUMMARY: Chromosomal DNA from 37 representing genetic species, groups and reference strains together with five leptospire isolates and were digested with the restriction endonucleases HI, l and RI. The Southern blots were hybridized with a biotinylated 1·5 kb 16S rDNA probe and gave 36 reproducible and unique patterns. With the exception of the type strain ( serovar RGA) and neotype strain (serovar Ictero no. 1) all the species and taxa examined could be differentiated from each other on the basis of their HI, l and RI restriction fragment length polymorphisms (RFLP). and reference strains were heterogeneous, whereas and were distinct and separate. Strains representing presented divergent RFLP patterns. A porcine isolate was identified to be Pomona.

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© Society for General Microbiology, 1993
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1993-08-01
2024-04-24
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Characterization of Leptospiraceae by 16S DNA restriction fragment length polymorphisms
Microbiology 139, 1681 (1993); https://doi.org/10.1099/00221287-139-8-1681
/content/journal/micro/10.1099/00221287-139-8-1681
/content/journal/micro/10.1099/00221287-139-8-1681
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