1887

Abstract

SUMMARY: Chromosomal DNA from 37 representing genetic species, groups and reference strains together with five leptospire isolates and were digested with the restriction endonucleases HI, l and RI. The Southern blots were hybridized with a biotinylated 1·5 kb 16S rDNA probe and gave 36 reproducible and unique patterns. With the exception of the type strain ( serovar RGA) and neotype strain (serovar Ictero no. 1) all the species and taxa examined could be differentiated from each other on the basis of their HI, l and RI restriction fragment length polymorphisms (RFLP). and reference strains were heterogeneous, whereas and were distinct and separate. Strains representing presented divergent RFLP patterns. A porcine isolate was identified to be Pomona.

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1993-08-01
2021-05-12
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References

  1. ANDERSEN J.K., SAUNDERS N.A. 1990; Epidemiological typing of Yersinia enterocolitica by analysis of restriction fragment length polymorphisms with a cloned ribosomal RNA gene.. Journal of Medical Microbiology 32:179–187
    [Google Scholar]
  2. BROSIUS J., PALMER M.L., KENNEDY P.J., NOLLER H.F. 1978; Complete nucleotide sequence of a 16S ribosomal gene from Escherichia coli.. Proceedings of the National Academy of Sciences of the United States of America 754801–4805
    [Google Scholar]
  3. CACCIAPUOTI B., CICERONI L., BARBINI D.A. 1991; Fatty acid profiles, a key for the classified of strains of the family Leptospiraceae.. International Journal of Systematic Bacteriology 41:295–300
    [Google Scholar]
  4. CINCO M., PETELIN N. 1970; Serogroups and serotypes in water- leptospira strains.. Tropical Geography and Medicine 22:237–244
    [Google Scholar]
  5. Cox N., JOHNSTON J., SZARKA Z., WRIGHT D.J.M., ARCHARD L.C. 1990; Characterization of an rRNA gene-specific cDNA probe: applications in bacterial identification.. Journal of General Microbiology 136:1639–1643
    [Google Scholar]
  6. DIESCH S.L., MCCULLOCH W.F., BRAUN J.L., ELLINGHAUSEN H.C. 1966; Leptospires isolated from frog kidneys. . Nature, London 209:939–940
    [Google Scholar]
  7. DOHERTY J.P., ADLER B., ROOD J.I., BILLINGTON S.J., FAINE S. 1989; Expression of two conserved leptospiral antigens in Escher-ichia coli.. Journal of Medical Microbiology 28:143–149
    [Google Scholar]
  8. EDWARDS U., ROGALL T., BLOCKER H., EMDE M., BOTTGER E.C. 1989; Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. . Nucleic Acids Research 17:7843–7853
    [Google Scholar]
  9. ELLINGHAUSEN H.C., MCCULLOUGH W.G. 1965; Nutrition of Leptospirapomona and growth of 13 other serotypes: Fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. . American Journal of Veterinary Research 26:45–51
    [Google Scholar]
  10. ELLIS W.A. 1990; Leptospirosis - a review of veterinary aspects. . Irish Veterinary News 12:6–12
    [Google Scholar]
  11. EVERARD C.O.R., EDWARDS C.N., WEBB G.B., WHITE H.ST.C., NICHOLSON G.D. 1984; The prevalence of severe leptospirosis among humans on Barbados.. Transactions of the Royal Society of Tropical Medicine and Hygiene 78:596–603
    [Google Scholar]
  12. VAN EYS G.J.J.M., ZAAL J., SCHOONE G.J., TERPSTRA W.J. 1988; DNA hybridization with hardjobovis-spoeiite, recombinant probes as a method for type discrimination of Leptospira interrogans.. Journal of General Microbiology 134:567–574
    [Google Scholar]
  13. VAN EYS G.J.J.M., GERRITSEN M.J., KORVER H., SCHOONE G.J., KROON C.C.M., TERPSTRA W.J. 1991; Characterization of serovars of the genus Leptospira by DNA hybridization with hardjobovis and icterohaemorrhagiae recombinant probes with special attention to serogroup Sejroe.. Journal of Clinical Microbiology 29:1042–1048
    [Google Scholar]
  14. FUKUNAGA M., MASUZAWA T., OKUZAKO N., MIFUCHI I., YANAGIHARA Y. 1990; Linkage of ribosomal genes in Leptospira.. Microbiology and Immunology 34:565–573
    [Google Scholar]
  15. GRAVEKAMP C., KORVER H., MONTGOMERY J., EVERARD C.O., CARRINGTON D., ELLIS W.A., TERPSTRA W.J. 1991; Leptospires isolated from toads and frogs on the Island of Barbados. . International Journal of Medical Microbiology 275:403–411
    [Google Scholar]
  16. GRIMONT F., GRIMONT P.A.D. 1986; Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.. Annales de l’Institut Pasteur Microbiology 137:165–175
    [Google Scholar]
  17. HERRMANN J.L., BELLENGER E., PEROLAT P., BARONTON G., SAINTGIRONS I. 1992; Pulsed-field gel electrophoresis of Notl digests of leptospiral DNA: a new rapid method of serovar identification. . Journal of Clinical Microbiology 30:1696–1702
    [Google Scholar]
  18. HOLMES B., COSTAS M., WOOD A.C., OWEN R.J., MORGAN D.D. 1990; . Differentiation of Achromobacter-like strains from human blood by DNA restriction endonuclease digest and ribosomal RNA gene probe patterns. . Epidemiology and Infection 105:541–551
    [Google Scholar]
  19. HOOKEY J.V. 1990; The detection of genetic variation in Leptospira interrogans serogroup icterohaemorrhagiae by ribosomal RNA gene restriction fragment patterns. . FEMS Microbiology Letters 72:329–336
    [Google Scholar]
  20. HOOKEY J.V. 1992; Detection of Leptospiraceae by amplification of 16S ribosomal DNA. . FEMS Microbiology Letters 90:267–274
    [Google Scholar]
  21. HOOKEY J.V., PALMER M.F. 1991; A comparative investigation and identification of Leptospira interrogans serogroup icterohaemorrhagiae strains by monoclonal antibody and DNA fingerprint analyses. . Zentralblatt für Bakteriologie 272:185–199
    [Google Scholar]
  22. HOVIND-HOUGEN K. 1979; Leptospiraceae, a new family to include Leptospira Noguchi 1917 and Leptonema gen. nov. . International Journal of Systematic Bacteriology 29:245–251
    [Google Scholar]
  23. HOVIND-HOUGEN K., ELLIS W. A., BIRCH-ADERSEN A. 1981; Leptospira parva sp. nov.; some morphological and biological characters. . Zentralblatt für Bakteriologie. Parasitenkunde Infektionskrankheiten und Hygiene Abteilung I Originale Reihe A 250:343–354
    [Google Scholar]
  24. JOHNSON R.C., FAINE S. 1984; Genus I. Leptospira Noguchi 1917, 755AL.. In Bergey’s Manual of Systematic Bacteriology 1 pp. 62–67 Edited by Krieg N. R., Holt J. G. Baltimore/London: Williams & Wilkins;
    [Google Scholar]
  25. KANAUJIA G.V., KATOCH V.M., SHIVANNAVAR C.T., SHARMA V.D., PATIL M.A. 1991; Rapid characterization of Mycobacterium fortuitum-chelonei complex by restriction fragment length polymorphism of ribosomal RNA genes.. FEMS Microbiology Letters 77:205–208
    [Google Scholar]
  26. KOBAYASHI Y., SADA E., TAMAI T. 1991; Serological studies on Leptospira strain Ictero No 1 using monoclonal antibodies.. Microbiology and Immunology 35:67–76
    [Google Scholar]
  27. KMETY E., DIKKEN H. 1988; Revised list of Leptospira serovars. I Alphabetical order and II Chronological order. In . International Committee on Systematic Bacteriology of the International Union of Microbiological Societies Subcommittee on the Taxonomy of Leptospira. Edited by Kmety E., Dikken H. Groningen: University Press;
    [Google Scholar]
  28. MARSHALL R.B., WILTON B.E., ROBINSON A.J. 1981; Identification of leptospira serovars by restriction endonuclease analysis.. Journal of Medical Microbiology 14:163–166
    [Google Scholar]
  29. MORGAN D.D., OWEN R.J. 1990; Use of DNA restriction endonuclease digest and ribosomal RNA gene probe patterns to fingerprint Helicobacter pylori and Helicobacter mustelae isolated from human and animal hosts. . Molecular and Cellular Probes 4:321–334
    [Google Scholar]
  30. NG L.-K., DILLON J.-A.R. 1991; Molecular fingerprinting of isolates of the genus Peptostreptococcus using rRNA genes from Escherichia coli and P. anaerobius.. Journal of General Microbiology 137:1323–1331
    [Google Scholar]
  31. OWEN R.J., BECK A., DAYAL P.A., DAWSON C. 1988; Detection of genomic variation in Providencia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons. . Journal of Clinical Microbiology 26:2161–2166
    [Google Scholar]
  32. PEROLAT P., GRIMONT F., REGNAULT B., GRIMONT P.A.D., FOURNIE E., THEVENET H., BARONTON G. 1990; rRNA gene restriction patterns of Leptospira-, a molecular typing system. . Research in Microbiology 141:159–171
    [Google Scholar]
  33. PICARD B., PICARD-PASQUIER N., KRISHNAMOORTHY R., GOULLET P. 1991; Characterization of highly virulent Escherichia coli strains by ribosomal DNA restriction fragment length polymorphism. . FEMS Microbiology Letters 82:183–188
    [Google Scholar]
  34. PICARD-PASQUIER N., PICARD B., HEERALAL S., KRISHNAMOORTHY R., GOULLET P. 1990; Correlation between ribosomal DNA polymorphism and electrophoretic enzyme polymorphism in Yersinia.. Journal of General Microbiology 136:1655–1666
    [Google Scholar]
  35. PRBHEIM L., PITCHER D., OWEN R., COOKSON B. 1991; Typing of methicillin resistant and susceptible Staphylococcus aureus strains by ribosomal RNA gene restriction patterns using a biotinylated probe. . European Journal of Clinical Microbiology and Infectious Diseases 10:428–436
    [Google Scholar]
  36. RAMADASS P., MARSHALL R.B. 1990; Species differentiation of Leptospira interrogans serovar hardjo strain Hardjobovis from strain Hardjoprajitno by DNA slot blot hybridisation.. Research in Veterinary Science 49:194–197
    [Google Scholar]
  37. RAMADASS P., JARVIS B.D.W., CORNER R.J., CINCO M., MARSHALL R.B. 1990; DNA relatedness among strains of Leptospira biflexa.. International Journal of Systematic Bacteriology 40:231–235
    [Google Scholar]
  38. RAMADASS P., JARVIS B.D.W., CORNER R.J., MARSHALL R.B. 1992; Genetic characterization of pathogenic Leptospira species by DNA hybridization. . International Journal of Systematic Bacteriology 42:215–219
    [Google Scholar]
  39. SAMBROOK J., FRITSCH E., MANIATIS T. 1989; Analysis of genomic DNA by Southern hybridization.. In Molecular Cloning: A Laboratory Manual pp. 9.31–9.59 Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  40. SAUNDERS N.A., HARRISON T.G., KACHWALLA N., TAYLOR A.G. 1988; Identification of species of the genus Legionella using a cloned rRNA gene from Legionella pneumophila.. Journal of General Microbiology 134:2363–2374
    [Google Scholar]
  41. SEGERS R.P.A.M., VANGESTEL J.A., VANEYS G.J.J.M., VANDERZEIJST B.A.M., GAASTRA W. 1992; Presence of putative sphingomyelinase genes among members of the family Leptospiraceae.. Infection and Immunity 60:1707–1710
    [Google Scholar]
  42. SOUTHERN E.M. 1979; Measurement of DNA length by gel electrophoresis.. Analytical Biochemistry 100:319–323
    [Google Scholar]
  43. STULL T.L., LIPUMA J.J., EDLIND D. 1988; A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA.. Journal of Infectious Diseases 157:280–286
    [Google Scholar]
  44. SULZER C.R. 1975 In Leptospiral Serotype Distribution List: According to Host and Geographic Area. Centre for Disease Control, Bureau of Laboratories, Atlanta, GA, U.S. Department of Health, Education and Welfare Public Health Service.
    [Google Scholar]
  45. TAYLOR J., GOYLEAY N. 1931; Leptospirosis in the Andamans.. Indian Medical Research Memoirs 20:1–90
    [Google Scholar]
  46. THIERMANN A.B., LEFEBVRE R.B. 1989; Restriction endonuclease analysis and other molecular techniques in identification and classification of Leptospira and other pathogens of veterinary importance.. In Nucleic Acid and Monoclonal Antibody Probes: Applications in Diagnostic Microbiology, pp. 145–183 Edited by Swaminathan B., Prakash G. New York: Marcel Dekker;
    [Google Scholar]
  47. THIERMANN A.B., HANDSAKER A.L., FOLEY J.W., WHITE F.H., KINGSCOTE B.F. 1986; Reclassification of North American isolates belonging to serogroups Mini and Sejroe by restriction endonuclease analysis. . American Journal of Veterinary Research 47:61–66
    [Google Scholar]
  48. WILLIAMS A.M., COLLINS M.D. 1991; DNA fingerprinting of Streptococus uberis based on polymorphism of DNA encoding rRNA. . Letters in Applied Microbiology 12:23–28
    [Google Scholar]
  49. WOESE C. 1987; Bacterial evolution. . Microbiological Reviews 51:221–271
    [Google Scholar]
  50. WOODWARD M.J., SULLIVAN G.T. 1991; Nucleotide sequence of a repetitive element isolated from Leptospira interrogans serovar hardjo type hardjo-bovis.. Journal of General Microbiology 137:1101–1109
    [Google Scholar]
  51. YAMAGUCHI T., ONO E., YANAGAWA R. 1988; Expression of antigen genes of Leptospira interrogans serovar canicola in Escherichia coli.. Microbiology and Immunity 32:1179–1187
    [Google Scholar]
  52. YASUDA P.H., STEIGERWALT A.G., SULZER K., KAUFMANN A., ROGERS F., BRENNER D.J. 1987; Deoxyribonucleic acid relatedness between serogroups an serovars in the family Leptospiraceae with proposals for seven new Leptospira species.. International Journal of Systematic Bacteriology 37:407–415
    [Google Scholar]
  53. ZUERNER R.L., BOLIN C.A. 1988; Repetitive sequence element cloned from Leptospira interrogans serovar hardjo type hardjo-bovis provides a sensitive diagnostic probe for bovine leptospirosis. . Journal of Clinical Microbiology 26:2495–2500
    [Google Scholar]
  54. ZUERNER R.L., BOLIN C.A. 1990; Nucleic acid probe characterizes Leptospira interrogans serovars by restriction fragment length polymorphisms.. Veterinary Microbiology 24:355–366
    [Google Scholar]
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