1887

Abstract

Summary: The mycelial growth of was examined at different water activities ( ) using glycerol as the osmoticum. Growth was optimal at 0.90 and restricted at 0.995 highlighting the xerophilic behaviour of Decreased produced an increase in the proportion of oleic acid (C) at the expense of the proportion of linoleic acid (C) of cellular phospholipids. The degree of unsaturation of phospholipid fatty acids showed a 20 % decrease between 0.995 and 0.80 of growth. Steady-state fluorescence anisotropy ( ) and fluorescence lifetime (τ) measurements for liposomes prepared from cellular phospholipids of and labelled with DPH (1,6-diphenyl-1,3,5-hexatriene) were made at 25 °C. The lipid order parameter ( describing molecular order) and the rotational correlation time (ϕ, describing molecular dynamics) were calculated from and τ data. Except at 0.995 , a decrease in was accompanied by increasing and values, indicating a rigidification of membranes, while ϕ values were not significantly different. Plots of order parameters and their first derivatives as a function of temperature exhibited break areas in the temperature range 20-48 °G. These large temperature ranges for lipid transitions could correspond to chain melting of complex lipid systems which made up the liposomes prepared from phospholipids of However, as decreased, the transition temperatures increased globally, between 0.97 and 0.90 .

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/content/journal/micro/10.1099/00221287-139-7-1653
1993-07-01
2021-04-18
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