1887

Abstract

Summary: Phytotoxins with potato-scab-inducing activity were produced by the pathogenic strain RB2 when grown on oatmeal agar medium or in oatmeal broth medium. Five compounds isolated from cell filtrates were identified as thaxtomin compounds 1-5, previously reported as produced by grown on potato slices. The optimum temperature for phytotoxin production in oatmeal broth medium was 28 °C. Production of thaxtomin A, the major product, was repressed at least 130-fold when RB2 was grown in oatmeal broth medium supplemented with 0.5% glucose. Thaxtomin A production was also repressed by tryptophan and tyrosine, precursors of which may be involved in feedback inhibition of early steps in biosynthesis. Phytotoxins were secreted by the organism when the cells reached late exponential to early stationary phases of culture growth. The time during growth at which each thaxtomin compound was produced suggests a pathway for the latter steps in thaxtomin biosynthesis.

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1993-07-01
2024-04-19
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References

  1. ADAMS M.J. 1975; Potato tuber lenticels: development and structure.. Annals of Applied Biology 79:265–273
    [Google Scholar]
  2. ARCHULA J.R., EASTON G.D. 1981; The cause of deep-pitted scab of potatoes. . American Potato Journal 51:35–43
    [Google Scholar]
  3. BROWN K.D., DOY C.H. 1963; End-product regulation of the general aromatic pathway in Escherichia coli W. . Biochimica et Biophysica Acta 77:170–172
    [Google Scholar]
  4. DAVIS J.R., GARNER J. 1978 Common Scab of Potato. University of Idaho Agriculture Experiment Station. Current Information Series, no. 386.
    [Google Scholar]
  5. DEMAIN A.L. 1989; Carbon source regulation of idiolite biosynthesis in actinomycetes.. In Regulation of Secondary Metabolism in Actinomycetes, pp. 127–134 Edited by Shapiro S. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  6. GOTO K. 1981; The relationship between common scab severity and reducing sugar contents in the peel of potato tubers.. Potato Research 24:171–176
    [Google Scholar]
  7. GROSS D.C. 1985; Regulation of syringomycin synthesis in Pseudomonas syringae pv. syringae and defined conditions for its production. . Journal of Applied Microbiology 58:167–174
    [Google Scholar]
  8. HARRISON M.D. 1962; Potato russet scab, its cause and factors affecting its development.. American Potato Journal 39:368–387
    [Google Scholar]
  9. HOOD D.W., HEIDSTRA R., SWOBODA U.K., HODGSON D.A. 1992; Molecular genetic analysis of proline and tryptophan biosynthesis in Streptomyces coelicolor A3(2): interaction between primary and secondary metabolism - a review. . Gene 115:5–12
    [Google Scholar]
  10. HOOKER W.J. 1981; Common scab.. In Compendium of Potato Diseases pp. 33–34 Edited by Hooker W. J. St. Paul, MN: American Phytopathological Society;
    [Google Scholar]
  11. HOPWOOD D.A., BIBB M.J., CHATER K.F., KIESER T., BRUTON C.J., KIESER H.M., LYDIATE D.J., SMITH C.P., WARD J.M., SCHREMPF H. 1985 Genetic Manipulation of Streptomyces - a Laboratory Manual. Norwich, UK: The John Innes Foundation;
    [Google Scholar]
  12. KING R.R., LAWRENCE C.H., CLARK M.C., CALHOUN L.A. 1989; Isolation and characterization of phytotoxins associated with Streptomyces scabies.. Journal of the Chemical Society (London) 13:849–850
    [Google Scholar]
  13. KING R.R., LAWRENCE C.H., CLARK M.C. 1991; Correlation of phytotoxin production with pathogenicity of Streptomyces scabies isolates from scab infected potato tubers.. American Potato Journal 68:675–680
    [Google Scholar]
  14. KING R.R., LAWRENCE C.H., CALHOUN L.A. 1992; Chemistry of phytotoxins associated with Streptomyces scabies, the causal organism of potato common scab. . Journal of Agricultural Food Chemistry 40:834–837
    [Google Scholar]
  15. KOLATTUKUDY P.E. 1980; Biopolyester membranes of plants: cutin and suberin. . Science 208:990–999
    [Google Scholar]
  16. KOLATTUKUDY P.E. 1981; Structure, biosynthesis and biodegradation of cutin and suberin. . Annual Review of Plant Physiology 32:539–567
    [Google Scholar]
  17. KOLATTUKUDY P.E. 1984; Biochemistry and function of cutin and suberin. . Canadian Journal of Botany 62:2918–2933
    [Google Scholar]
  18. LAMBERT D.H., LORIA R. 1989; Streptomyces scabies sp. nov., nom. rev. . International Journal of Systematic Bacteriology 39:387–392
    [Google Scholar]
  19. LAUER F.I. 1977; Tubers from leaf-bud cuttings: a tool for potato seed certification and breeding. . American Potato Journal 54:457–464
    [Google Scholar]
  20. LAWRENCE C.H., CLARK M.C., KING R.R. 1990; Induction of common scab symptoms in aseptically cultured potato tubers by the vivo toxin, thaxtomin. . Phytopathology 80:606–608
    [Google Scholar]
  21. MURASHIGE T., SKOOG F. 1962; A revised medium for rapid growth and bioassays with tobacco tissue cultures. . Physiologia Plantarum 15:473
    [Google Scholar]
  22. NUSKE J., FRITSCHE W. 1989; Phaseolotoxin production by Pseudomonas syringae pv. phaseolicola: the influence of temperature. . Journal of Basic Microbiology 29:441–447
    [Google Scholar]
  23. SHAPIRO S. 1989; Nitrogen assimilation in actinomycetes and the influence of nitrogen nutrition on actinomycete secondary metabolism.. In Regulation of Secondary Metabolism in Actinomycetes pp. 135–211 Edited by Shapiro S. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  24. SHIRLING E.B., GOTTLIEB D. 1966; Methods of characterization of Streptomyces species. . International Journal of Systematic Bacteriology 16:313–340
    [Google Scholar]
  25. SLACK S.A. 1992; A look at potato leafroll virus and PVY: past, present and future. . Valley Potato Grower 57:35–39
    [Google Scholar]
  26. SMITH L.C., RAVEL J.M., LAX S.R., SHIVE W. 1962; The control of 3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthesis by phenylalanine and tyrosine. . Journal of Biological Chemistry 237:3566–3570
    [Google Scholar]
  27. UNITED STATES DEPARTMENT OF AGRICULTURE : AGRICULTURE SCIENCE AND EDUCATION ADMINISTRATion 1982; Composition of foods: breakfast cereals, raw, processed and prepared.. In Agriculture Handbook No. 8-8 pp. 137–138 Washington DC: United States Government Printing Office.;
    [Google Scholar]
  28. UNITED STATES DEPARTMENT OF AGRICULTURE: HUMAN NUTRITION INFORMATION SERVICE 1984; Composition of foods: vegetables and vegetable products: raw, processed and prepared.. In Agriculture Handbook No. 8-11 pp. 294–295 Washington DC: United States Government Printing Office;
    [Google Scholar]
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