%0 Journal Article %A Pal, Sukumar %A Cheng, Xun %A Peterson, Ellena M. %A de la Maza, Luis M. %T Mapping of a surface-exposed B-cell epitope to the variable sequent 3 of the major outer-membrane protein of Chlamydia trachomatis %D 1993 %J Microbiology, %V 139 %N 7 %P 1565-1570 %@ 1465-2080 %R https://doi.org/10.1099/00221287-139-7-1565 %I Microbiology Society, %X Summary: A B-cell epitope, AEFPLDIT, was located to the variable sequent 3 of the major outer-membrane protein (MOMP) using the monoclonal antibody L3-1, raised to the Chlamydia trachomatis serovar L3 MOMP. By Western blot and inclusion immunofluorescence assay the monoclonal antibody recognized all the C complex and C-related complex serovars of C. trachomatis, except serovar C. Dot-blot and ELISA data using native elementary bodies indicated that the epitope was surface exposed. The monoclonal antibody, at concentrations of 10 and 100 μg per 107 chlamydial inclusion-forming units, was able to neutralize the infectivity of chlamydia in an in vivo assay but did not neutralize chlamydia in vitro or in a mouse toxicity assay. A peptide corresponding to the variable sequent 3 has previously been shown to also elicit a T-cell response; thus, careful consideration should be given to inclusion of this region of the major outer-membrane protein in a subunit vaccine. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-7-1565