1887

Abstract

Summary: Insertion element IS occurs in multiple copies throughout the genome, and the variability of its insertion sites is the basis for the IS restriction fragment length polymorphism (RFLP) method for typing. We describe a novel gene amplification method to assess the variability of the location of IS A unilateral-nested polymerase chain reaction and hybridization procedure was used to measure the variability in the distances between IS elements and copies of a major polymorphic tandem repeat sequence of The pattern of amplicons produced could be used to cluster epidemiologically related strains of into groups which correlated with the groups formed using IS-RFLP typing. Reliable patterns can be generated directly from sputum specimens as well as from cultures. We designated the novel method as IS-ampliprinting.

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1993-07-01
2021-07-27
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References

  1. Barnes P. F., Bloch A. B., Davidson P. T., Snider D. E. JR 1991; Tuberculosis in patients with human immunodeficiency virus infection.. New England Journal of Medicine 324:1644–1650
    [Google Scholar]
  2. Cave M. D., Eisenach K. D., Mcdermott P. F., Bates J. H., Crawford J. T. 1991; IS6110: conservation of sequence in the Mycobacterium tuberculosis complex and its utilization in DNA fingerprinting.. Molecular and Cellular Probes 5:73–80
    [Google Scholar]
  3. Edlin B. R., Tokars J. I., Grieco M. H., Crawford J. T., Williams J., Sordillo E. M., Ong K. R., Kilburn J. O., Dooley S. W., Castro K. G., Jarvis W. R., Holmberg S. D. 1992; An outbreak of multidrug-resistant tuberculosis among hospitalized patients with the acquired immunodeficiency syndrome.. New England Journal of Medicine 326:1514–1521
    [Google Scholar]
  4. Hermans P. W. M., Van Soolingen D., Dale J. W., Schuitema A. R. J., Mcadam R. A., Catty D., Van Embden J. D. A. 1990; Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis.. Journal of Clinical Microbiology 28:2051–2058
    [Google Scholar]
  5. Hermans P. W. M., Van Soolingen D., Van Embden J. D. A. 1992; Characterization of a major polymorphic tandem repeat in Mycobacterium tuberculosis and its potential use in the epidemiology of Mycobacterium kansasii and Mycobacterium gordonae.. Journal of Bacteriology 174:4157–4165
    [Google Scholar]
  6. Kent B. B., Kubica K. G. P. 1985 Public Health Mycobacteri- ology: a Guide for the Level III Laboratory. Atlanta, Georgia: US Department of Health and Human Services, Centers for Disease Control.;
    [Google Scholar]
  7. Mcadam R. A., Hermans P. W. M., Van Soolingen D., Zainuddin A. F., Catty D., Van Embden J. D. A., Dale J. W. 1990; Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the I S3 family.. Molecular Microbiology 4:1607–1613
    [Google Scholar]
  8. Mullis K. B., Faloona F. A. 1987; Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.. Methods in Enzymology 155:335–350
    [Google Scholar]
  9. Otal I., Martin C., Vincent-Levy-Frebault V., Thierry D., Gicquel B. 1991; Restriction fragment length polymorphism analysis using IS6110 as an epidemiological marker in tuberculosis.. Journal of Clinical Microbiology 29:1252–1254
    [Google Scholar]
  10. Pearson M. L., Jereb J. A., Frieden T. R., Crawford J. T., Davis B. J., Dooley S. W., Jarvis W. R. 1992; Nosocomial transmission of multidrug-resistant Mycobacterium tuberculosis: a risk to patients and healthcare workers.. Annals of Internal Medicine 117:191–196
    [Google Scholar]
  11. Plikaytis B. B., Eisenach K. D., Crawford J. T., Shinnick T. M. 1991; Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis by a polymerase chain reaction assay.. Molecular and Cellular Probes 5:215–219
    [Google Scholar]
  12. Plikaytis B. B., Plikaytis B. D., Yakrus M. A., Butler W. R., Woodley C. L., Silcox V. A., Shinnick T. M. 1992; Differentiation of slowly growing Mycobacterium species, including Mycobacterium tuberculosis, by gene amplification and restriction fragment length polymorphism analysis.. Journal of Clinical Microbiology 30:1815–1822
    [Google Scholar]
  13. Shinnick T. M. 1987; The 65-kilodalton antigen of Mycobacterium tuberculosis.. Journal of Bacteriology 169:1080–1088
    [Google Scholar]
  14. Thierry D., Cave M. D., Eisenach K. D., Crawford J. T., Bates J. H., Gicquel B., Guesdon J. L. 1990; IS6110 an IS-like element of Mycobacterium tuberculosis complex.. Nucleic Acids Research 18:188
    [Google Scholar]
  15. Versalovic J., Koeuth T., Lupski J. R. 1991; Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.. Nucleic Acids Research 19:6823–6831
    [Google Scholar]
  16. Wilson K. 1990; Preparation of genomic DNA from bacteria.. In Current Protocols in Molecular Biology 1: pp. 2.4.1–2.4.2. Edited by Ausubel F. M., Brent R., Kinston R. E., Moore D. D., Seidman I. G., Smith J. A., Struhl K. New York: Wiley Interscience.;
    [Google Scholar]
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