Summary: Insertion element IS occurs in multiple copies throughout the genome, and the variability of its insertion sites is the basis for the IS restriction fragment length polymorphism (RFLP) method for typing. We describe a novel gene amplification method to assess the variability of the location of IS A unilateral-nested polymerase chain reaction and hybridization procedure was used to measure the variability in the distances between IS elements and copies of a major polymorphic tandem repeat sequence of The pattern of amplicons produced could be used to cluster epidemiologically related strains of into groups which correlated with the groups formed using IS-RFLP typing. Reliable patterns can be generated directly from sputum specimens as well as from cultures. We designated the novel method as IS-ampliprinting.


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