1887

Abstract

Summary: Many strains of oral streptococci secrete glucosyltransferases (GTFs) that polymerize sucrose into glucans that form an integral part of the plaque matrix on the tooth surface. Recently, we reported the cloning of two closely linked GTF-encoding genes ( and ) from ATCC 25975 as well as the sequence of , which encodes a primer-dependent GTF that synthesizes an insoluble product (a GTF-I). In this communication we report the sequence of which encodes a primer-dependent GTF that synthesizes a soluble product (a GTF-S), as well as the sequence of a small downstream open reading frame of unknown function. The deduced sequence of GtfK was compared with those of seven other streptococcal Gtfs and an unrooted phylogenetic tree constructed. This analysis suggested that Gtfs with similar product specificities do not form phylogenetic clusters and was consistent with currently accepted phylogenetic schemes. The tree was tested by constructing a series of “sub-trees” from different blocks of the alignment. Evidence was obtained for recombination events involving and from GS-5, and from as well as the genes from and The recombination events between and and between the two genes, were confirmed by examining divergences at silent sites.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-7-1511
1993-07-01
2021-04-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/7/mic-139-7-1511.html?itemId=/content/journal/micro/10.1099/00221287-139-7-1511&mimeType=html&fmt=ahah

References

  1. Abo H., Matsumura T., Kodama T., Ohta H., Fukui K., Kato K., Kagawa H. 1991; Peptide sequences for sucrose splitting and glucan binding within Streptococcus sobrinus glucosyltransferase (water-insoluble glucan synthetase).. Journal of Bacteriology 173:989–996
    [Google Scholar]
  2. Aoki H., Shiroza T., Hayakawa M., Sato S., Kuramitsu H. K. 1986; Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis.. Infection and Immunity 53:587–594
    [Google Scholar]
  3. Banas J. A., Russell R. R. B., Ferretti J. J. 1990; Sequence analysis of the gene for the glucan-binding protein of Streptococcus mutans Ingbritt.. Infection and Immunity 58:667–673
    [Google Scholar]
  4. Beighton D., Russell R. R. B., Hayday H. 1981; The isolation and characterization of Streptococcus mutans serotype h from dental plaque of monkeys {Macaco fascicular is).. Journal of General Microbiology 124:271–279
    [Google Scholar]
  5. Bentley R. W., Leigh J. A., Collins M. D. 1991; Intrageneric structure of Streptococcus based on comparative analysis of small- subunit rRNA sequences.. International Journal of Systematic Bacteriology 41:487–494
    [Google Scholar]
  6. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  7. Chia J.-S., Hsu T.-Y., Teng L. J., Chen J. Y., Hahn L. J., Yang C. S. 1991; Glucosyltransferase gene polymorphism among Strep-tococcus mutans strains.. Infection and Immunity 59:1656–1660
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap.. Evolution 39:783–791
    [Google Scholar]
  9. Ferretti J. J., Gilpin M. L., Russell R. R. B. 1987; Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28.. Journal of Bacteriology 169:4271–4278
    [Google Scholar]
  10. Fitzgerald R. J., Keyes P. H., Stoudt T. H., Spinell D. M. 1968; The effects of a dextranase preparation on plaque and caries in hamsters, a preliminary report.. Journal of the American Dental Association 76:301–340
    [Google Scholar]
  11. Gibbons R. J. 1968; Formation and significance of bacterial polysaccharides in caries aetiology.. Caries Research 2:164–171
    [Google Scholar]
  12. Giffard P. M., Simpson C. L., Milward C. P., Jacques N. A. 1991; Molecular characterization of a cluster of at least two glucosyltransferase genes in Streptococcus salivarius ATCC 25975.. Journal of General Microbiology 137:2577–2593
    [Google Scholar]
  13. Gilmore K. S., Russell R. R. B., Ferretti J. J. 1990; Analysis of the Streptococcus downei gtfS gene, which specifies a glucosyl-transferase that synthesizes soluble glucans.. Infection and Immunity 58:2452–2458
    [Google Scholar]
  14. Gough J. A., Murray N. E. 1983; Sequence diversity among related genes for recognition of specific targets in DNA molecules.. Journal of Molecular Biology 166:1–19
    [Google Scholar]
  15. Hanada N., Kuramitsu H. K. 1988; Isolation and characterization of the Streptococcus mutans gtfC gene, coding for synthesis of both soluble and insoluble glucans.. Infection and Immunity 56:1999–2005
    [Google Scholar]
  16. Von HEIJNE G. 1983; Patterns of amino-acids near signal sequence cleavage sites.. European Journal of Biochemistry 133:17–21
    [Google Scholar]
  17. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.. Gene 28:351–359
    [Google Scholar]
  18. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTALV: improved software for multiple sequence alignment.. Computer Applications in the Biosciences (CABIOS) 8:189–191
    [Google Scholar]
  19. Honda O., Kato C., Kuramitsu H. K. 1990; Nucleotide sequence of the Streptococcus mutans gtfD gene.. Journal of General Microbiology 136:2099–2105
    [Google Scholar]
  20. Kimura M. 1983 The Neutral Theory of Molecular Evolution. Cambridge: Microbiology Society;
    [Google Scholar]
  21. Kobayashi S., Koga K., Hayashida O., Nakano Y., Hasegawa Y. 1989; Glucan-binding domain of a glucosyltransferase from Streptococcus sobrinus: isolation of a 55-kilodalton peptide from a trypsin digest of glucosyltransferase prebound to insoluble glucan.. Infection and Immunity 57:2210–2213
    [Google Scholar]
  22. Ludwig W., Seewaldt E., Kilpper-BÄLZ R., Schleifer K., Magrum L., Woese C. R., Fox G. E., Stackebrandt E. 1985; The phylogenetic position of Streptococcus and Enterococcus.. Journal of General Microbiology 131:543–551
    [Google Scholar]
  23. Maniatis T., Fitsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  24. Miller J. H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Mooser G., Iwaoka K. R. 1989; Sucrose 6-α-D-glucosyltransferase: characterization of glycosyl-enzyme complex.. Biochemistry 28:443–449
    [Google Scholar]
  26. Mooser G., Wong C. 1988; Isolation of a glucan-binding domain of glucosyltransferase (1,6-α-glucan synthase) from Streptococcus sobrinus.. Infection and Immunity 56:880–884
    [Google Scholar]
  27. Mooser G., Hefta S. A., Paxton R. J., Shively J. E., Lee T. D. 1991; Isolation and sequence of an active-site peptide containing a catalytic aspartic acid from two Streptococcus sobrinus α-glucosyltransferases.. Journal of Biological Chemistry 266:8916–8922
    [Google Scholar]
  28. Murray N. E., Brammar W. J., Murray K. 1977; Lambdoid phages that simplify the recovery of in vitro recombinants.. Molecular and General Genetics 150:53–61
    [Google Scholar]
  29. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison.. Proceedings of the National Academy of Sciences of the United States of America 852444–2448
    [Google Scholar]
  30. Perler F., Efstratiafis A., Lomedico P., Gilbert W., Kolodner R., Dodgson J. 1980; The evolution of genes: the chicken preproinsulin gene.. Cell 20:555–566
    [Google Scholar]
  31. Pitty L. J., Giffard P. M., Gilpin M. L., Russell R. R. B., Jacques N. A. 1989; Cloning and expression of glycosyltransferase activities from Streptococcus salivarius.. Journal of Dental Research 68:1681–1682
    [Google Scholar]
  32. RÖlla G., Ciardi J. E., Eggen K. H., Bowen W. F., Afseth J. 1983; Free glucosyl- and fructosyl-transferase in human saliva and absorption of these enzymes to teeth in vivo.. In Proceedings: Glucosyltransferase, Glucans, Sucrose and Dental Caries. Special Supplement to Chemical Sciences, pp. 21–29 Edited by Doyle J. R., Ciardi J. E. Oxford: IRL Press;
    [Google Scholar]
  33. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees.. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  34. Sanger F., Nicklen S., Coulsen A. R. 1977; DNA sequencing with chain terminating inhibitors.. Proceedings of the National Academy of Sciences of the United States of America 745463–5467
    [Google Scholar]
  35. Schleifer K. H., Kilpper-BÄlz R. 1987; Molecular and chemo- taxonomic approaches to the classification of streptococci, enterococci, and lactococci: a review.. Systematic and Applied Microbiology 10:1–19
    [Google Scholar]
  36. Schleifer K. H., Kilpper-BÄlz R., Kraus J., Gehring F. 1984; Relatedness and classification of Streptococcus mutans and ‘mutans-like’ streptococci.. Journal of Dental Research 63:1047–1050
    [Google Scholar]
  37. Shiroza T., Ueda S., Kuramitsu H. K. 1987; Sequence analysis of the gtfB gene from Streptococcus mutans GS-5.. Journal of Bacteriology 169:4263–4270
    [Google Scholar]
  38. Ueda S., Shiroza T., Kuramitsu H. K. 1988; Sequence analysis of the gtfC gene from Streptococcus mutans GS-5.. Gene 69:101–109
    [Google Scholar]
  39. Walker G. J. 1978; Dextrans.. International Reviews in Biochemistry 16:75–126
    [Google Scholar]
  40. Walker G. J., Jacques N. A. 1987; Polysaccharides of oral streptococci.. In Sugar Transport and Metabolism in Gram-positive Bacteria pp. 39–68 Edited by Reizer J., Peterkofsky A. Chichester: Ellis Horwood;
    [Google Scholar]
  41. Wren B. W. 1991; A family of clostridial and streptococcal ligandbinding proteins with conserved C-terminal repeat sequences.. Molecular Microbiology 5:797–803
    [Google Scholar]
  42. Yamashita Y., Bowen W. H., Kuramitsu H. K. 1992; Molecular analysis of a Streptococcus mutans strain exhibiting polymorphism in the tandem gtfB and gtfC genes.. Infection and Immunity 60:1618–1624
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-7-1511
Loading
/content/journal/micro/10.1099/00221287-139-7-1511
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error