1887

Abstract

Summary: The 2.55 kb plasmid, pRJ5, confers constitutive resistance to macrolide–lincosamide–streptogramin B (MLS) antibiotics. pRJ5 is nearly identical to the inducible MLS resistance plasmid pT48, and has homology with the plasmids pE194 and pSN2. The III-C and/or d-B fragments were required for stable maintenance of the plasmid and probably carry . Plasmids pRJ5 and pT48 were shown to belong to the same incompatibility group, Inc12 (L). DNA sequencing showed that pRJ5 contains a 28 bp direct tandem duplication in the leader/attenuator region of This is likely to change the secondary structure of the methylase mRNA, allowing constitutive expression of The type of mutation found on plasmid pRJ5 is different from those observed in similar 2.5 kb constitutive MLS-resistance plasmids isolated from other Gram-positive bacteria, including staphylococci.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-7-1461
1993-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/7/mic-139-7-1461.html?itemId=/content/journal/micro/10.1099/00221287-139-7-1461&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1990 Current Protocols in Molecular Biology. New York: John Wiley and Sons;
    [Google Scholar]
  2. Bastos M. C. F., Murphy E. 1988; Transposon Tn554 encodes three products required for transposition.. EMBO Journal 7:2935–2941
    [Google Scholar]
  3. Bastos M. C. F., Bonaldo M. C., Penido E. G. C. 1980; Constitutive erythromycin resistance plasmid in Staphylococcus aureus.. Journal of General Microbiology 121:513–516
    [Google Scholar]
  4. Byeon W.-H., Weisblum B. 1990; Replication genes of plasmid pE 194-cop and repF: transcripts and encoded proteins.. Journal of Bacteriology 172:5892–5900
    [Google Scholar]
  5. Catchpole I., Dyke K. G. H. 1990; A Staphylococcus aureus plasmid that specifies constitutive macrolide-lincosamide- streptogramin B resistance contains a novel deletion in the ermC attenuator.. FEMS Microbiology Letters 69:43–48
    [Google Scholar]
  6. Catchpole I., Dyke K. G. H. 1991; Replication mutants of Staphylococcus aureus macrolide-lincosamide-streptogramin B resistance plasmid pT48.. Molecular Microbiology 5:959–968
    [Google Scholar]
  7. Catchpole I., Thomas C., Davies A., Dyke K. G. H. 1988; The nucleotide sequence of Staphylococcus aureus plasmid pT48 conferring inducible macrolide-lincosamide-streptogramin B resistance and comparison with similar plasmids expressing constitutive resistance.. Journal of General Microbiology 134:697–709
    [Google Scholar]
  8. Dubnau D. 1984; Translational attenuation: the regulation of bacterial resistance to the macrolide-regulation of bacterial resistance to the macrolide-lincosamide-streptogramin B antibiotics.. CRC Critical Reviews in Biochemistry 16:103–132
    [Google Scholar]
  9. Giambiagi-MARVAL M., Mafra M. A., Penido E. G. C., Bastos M. C. F. 1990; Distinct groups of plasmids correlated with bacteriocin production in Staphylococcus aureus.. Journal of General Microbiology 136:1591–1599
    [Google Scholar]
  10. Gruss A., Ehrlich S. D. 1989; The family of highly interrelated single-stranded deoxyribonucleic acid plasmids.. Microbiological Reviews 53:231–241
    [Google Scholar]
  11. Gruss A., Ross H., Novick R. P. 1989; Functional analysis of a palindromic sequence required for normal replication of several staphylococcal plasmids.. Proceedings of the National Academy of Sciences of the United States of America 842165–2169
    [Google Scholar]
  12. Gryczan T., Grandi G., Hahn J., Grandi R., Dubnau D. 1980; Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin-induced drug resistance.. Nucleic Acids Research 8:6081–6097
    [Google Scholar]
  13. Hahn J., Grandi G., Gryczan T., Dubnau D. 1982; Translational attenuation of ermC: a deletion analysis.. Molecular and General Genetics 186:204–216
    [Google Scholar]
  14. Horinouchi S., Weisblum B. 1980; Post-transcriptional modifi-cation of mRNA conformation: mechanisms that regulate erythromycin-induced resistance.. Proceedings of the National Academy of Sciences of the United States of America 777079–7083
    [Google Scholar]
  15. Horinouchi S., Weislum B. 1982; Nucleotide sequence and functional map of pE194 a plasmid that specifies inducible resistance to macrolide, lincosamide and streptogramin-type B antibiotics.. Journal of Bacteriology 150:804–814
    [Google Scholar]
  16. Iordanescu S. 1976; Three distinct plasmids originating in the same Staphylococcus aureus strain.. Archives of Roumanian Experimental Pathology and Microbiology 35:111–118
    [Google Scholar]
  17. Iordanescu S., Surdeanu M. 1980; New incompatibility groups for Staphylococcus aureus plasmids.. Plasmid 4:256–260
    [Google Scholar]
  18. Khan S., Novick R. P. 1982; Structural analysis of plasmid pSN2 in Staphylococcus aureus: no involvement in enterotoxin B production.. Journal of Bacteriology 149:642–649
    [Google Scholar]
  19. Lampson B., Parisi J. 1986; Naturally occurring Staphylococcus epidermidis plasmid expressing constitutive macrolide-lincosamide-streptogramin B resistance contains a deleted attenuator.. Journal of Bacteriology 166:479–483
    [Google Scholar]
  20. Lyon B. R., Skurray R. 1987; Antimicrobial resistance of Staphylococcus aureus: genetic basis.. Microbiological Reviews 51:88–134
    [Google Scholar]
  21. Mayford M., Weisblum B. 1989; Conformation alterations in the ermC transcript in vivo during induction.. EMBO Journal 8:4307–4314
    [Google Scholar]
  22. Monod M., Denoya C., Dubnau D. 1986; Sequence and properties of pIM13 a macrolide-lincosamide-streptogramin B resistance plasmid from Bacillus subtilis.. Journal of Bacteriology 167:138–147
    [Google Scholar]
  23. Murphy E., Phillips S., Edelman I., Novick R. P. 1981; TnJ54: isolation and characterization of plasmid insertions.. Plasmid 5:292–305
    [Google Scholar]
  24. Novick R. P. 1967; Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus.. Virology 33:155–166
    [Google Scholar]
  25. Novick R. P. 1989; Staphylococcal plasmids and their replication.. Annual Reviews of Microbiology 43:537–565
    [Google Scholar]
  26. Novick R. P., Edelman I., Schwesinger M., Gruss A., Swanson E., Pattee P. 1979a; Genetic translocation in Staphylococcus aureus.. Proceedings of the National Academy of Sciences of the United States of America 76:400–404
    [Google Scholar]
  27. Novick R. P., Murphy E., Gryczan T. J., Baron E., Edelman I. 1979b; Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps.. Plasmid 2:109–129
    [Google Scholar]
  28. Projan S., Monod M., Narayanan C., Dubnau D. 1987; Replication properties of pIM13 a naturally occurring plasmid found in Bacillus subtilis, and of its close relative pE5 a plasmid native to Staphylococcus aureus.. Journal of Bacteriology 169:5131–5139
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. 1977; DNA sequencing with chain-terminating inhibitors.. Proceedings of the National Academy of Sciences of the United States of America 745463–5467
    [Google Scholar]
  31. Shivakumar A. G., Hahn J., Grandi G., Kozlov Y., Dubnau D. 1980; Posttranscript regulation of an erythromycin resistance protein specified by plasmid pE194.. Proceedings of the National Academy of Sciences of the United States of America 773903–3907
    [Google Scholar]
  32. Sozhamannan S., Dabert P., Moretto V., Ehrlich S. D., Gruss A. 1990; Plus-origin mapping of single-stranded DNA plasmid pE194 and nick site homologies with other plasmids.. Journal of Bacteriology 172:4543–4548
    [Google Scholar]
  33. Te RIELE H., Michel B., Ehrlich S. D. 1986; Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus.. Proceedings of the National Academy of Sciences of the United States of America 832541–2545
    [Google Scholar]
  34. Weisblum B., Graham M., Gryczan T., Dubnau D. 1979; Plasmid copy number control: isolation and characterization of high copy number mutants of plasmid pE194.. Journal of Bacteriology 137:635–643
    [Google Scholar]
  35. Zuker M., Stiegler P. 1981; Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.. Nucleic Acids Research 9:133–148
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-7-1461
Loading
/content/journal/micro/10.1099/00221287-139-7-1461
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error