1887

Abstract

Summary: The 2.55 kb plasmid, pRJ5, confers constitutive resistance to macrolide–lincosamide–streptogramin B (MLS) antibiotics. pRJ5 is nearly identical to the inducible MLS resistance plasmid pT48, and has homology with the plasmids pE194 and pSN2. The III-C and/or d-B fragments were required for stable maintenance of the plasmid and probably carry . Plasmids pRJ5 and pT48 were shown to belong to the same incompatibility group, Inc12 (L). DNA sequencing showed that pRJ5 contains a 28 bp direct tandem duplication in the leader/attenuator region of This is likely to change the secondary structure of the methylase mRNA, allowing constitutive expression of The type of mutation found on plasmid pRJ5 is different from those observed in similar 2.5 kb constitutive MLS-resistance plasmids isolated from other Gram-positive bacteria, including staphylococci.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-7-1461
1993-07-01
2020-01-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-7-1461
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error