1887

Abstract

Summary: Using pulsed-field gel electrophoresis (PFGE), we have demonstrated clonal variation in the size of chromosome XII in a diploid strain of X2180-2D. The sizes of the two chromosome XII homologues were very different: 2600 (L-type) and 1450 kb (S-type). The frequency with which we detected clonal size variation in the diploid, compared to that of the parental clones, was about 15-50% of the progeny clones and the range of the size variation of the homologues was 2580-2680 kb (L-type) and 1340-1500 kb (S-type), respectively. The homologue of the L-type appeared to be more frequently variable than that of the S-type. The size variation was shown to be derived from size changes in the rDNA cluster region, which is present in chromosome XII, by digesting the chromosome with I, whose cutting site is not present in a rDNA repeat unit, and hybridizing to rDNA probes. The clonal size variation was also investigated in haploids from spores after meiosis. The L-type and S-type chromosomes segregated 2:2 in an ascus and the sizes of all the S-type chromosomes were shifted up, compared to the original diploid, though the L-type ones were stable. The S-type sizes of 1340, 1450 and 1780 kb in the original diploids changed into the ranges of 1475-1610 kb, 1520-1680 kb and 1820-2010 kb, respectively, in the segregants. Furthermore, we observed that the size of S-type chromosomes in haploid cells was gradually increasing in mitosis during successive subcultures. The rDNA units appeared to be amplified on the S-type chromosome.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-7-1409
1993-07-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/7/mic-139-7-1409.html?itemId=/content/journal/micro/10.1099/00221287-139-7-1409&mimeType=html&fmt=ahah

References

  1. Asakura K., Iwaguchi S.-I., Homma M., Sukai T., Higashide K., Tanaka K. 1991; Electrophoretic karyotypes of clinically isolated yeasts of Candida albians and C. glabrata.. Journal of General Microbiology 137:2531–2538
    [Google Scholar]
  2. Christman M. F., Dietrich F. S., Fink G. R. 1988; Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II.. Cell 55:413–425
    [Google Scholar]
  3. Chu G., Vollrath D., Davis R. W. 1986; Separation of large DNA molecules by contour-clamped homogeneous electric fields.. Science 234:1582–1585
    [Google Scholar]
  4. Cramer J. H., Bhargava M. M., Halvorson H. O. 1972; Isolation and characterization of gamma DNA of Saccharomyces cerevisiae.. Journal of Molecular Biology 71:11–20
    [Google Scholar]
  5. Doi M., Homma M., Chindamporn A., Tanaka K. 1992; Estimation of chromosome number and size by pulsed-field gel electrophoresis (PFGE) in medically important Candida species.. Journal of General Microbiology 138:2243–2251
    [Google Scholar]
  6. Gottlieb S., Esposito R. E. 1989; A new role for a yeast transcriptional silencer gene, SIR2 in regulation of recombination in ribosomal DNA.. Cell 56:771–776
    [Google Scholar]
  7. Hawley R. S., Marcus C. H. 1989; Recombinational controls of rDNA redundancy in Drosophila.. Annual Review of Genetics 23:87–120
    [Google Scholar]
  8. Iwaguchi S.-I., Homma M., Tanaka K. 1990; Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans.. Journal of General Microbiology 136:2433–2442
    [Google Scholar]
  9. Iwaguchi S.-I., Homma M., Tanaka K. 1992; Clonal variation of chromosome size derived from the rDNA cluster region in Candida albicans.. Journal of General Microbiology 138:1177–1184
    [Google Scholar]
  10. Johnston J. R., Curran L., Contopoulou R. C., Mortimer R. K. 1989; Electrophoretic karyotyping of commercial brewing and distilling strains of Saccharomyces and of other yeasts (Seventh International Symposium on Yeasts).. Yeast 5:S255–S259
    [Google Scholar]
  11. De Jonge P., De Jongh F. C. M., Meijers R., Steensma H. Y., Scheffers W. A. 1986; Orthogonal-field-alternation gel electro-phoresis banding patterns of DNA from yeasts.. Yeast 2:193–204
    [Google Scholar]
  12. Kaback D. B., Halvorson H. O. 1977; Magnification of genes coding for ribosomal RNA in Saccharomyces cerevisiae. . Proceedings of the National Academy of Sciences of the United States of America 741177–1180
    [Google Scholar]
  13. Kaback D. B., Bhargava M. M., Halvorson H. O. 1973; Location and arrangement of genes coding for ribosomal RNA in Saccharomyces cerevisiae.. Journal of Molecular Biology 79:735–739
    [Google Scholar]
  14. Kaback D. B., Halvorson H. O., Rubin G. M. 1976; Location and magnification of 5S RNA genes in Saccharomyces cerevisiae.. Journal of Molecular Biology 107:385–390
    [Google Scholar]
  15. Keil R. L., Roeder G. S. 1984; Cis-acting, recombination-stimulating activity in a fragment of the ribosomal DNA of S. cerevisiae.. Cell 39:377–386
    [Google Scholar]
  16. Kuroiwa T., Kojima H., Miyakawa I., Sando N. 1984; Meiotic karyotype of the yeast Saccharomyces cerevisiae.. Experimental Cell Research 153:259–265
    [Google Scholar]
  17. Linskens M. H. K., Huberman J. A. 1988; Organization of replication of ribosomal DNA in Saccharomyces cerevisiae.. Molecular and Cellular Biology 8:4927–4935
    [Google Scholar]
  18. Long E. O., Dawid I. B. 1980; Repeated genes in eukaryotes.. Annual Review of Biochemistry 49:727–764
    [Google Scholar]
  19. Magee B. B., Magee P. T. 1987; Electrophoretic karyotypes and chromosome numbers in Candida species.. Journal of General Microbiology 133:425–430
    [Google Scholar]
  20. Merz W. G. 1990; Candida albicans strain delination.. Clinical Microbiology Reviews 3:321–334
    [Google Scholar]
  21. Mortimer R. K., Schild D. 1985; Genetic map of Saccharomyces cerevisiae, edition 9.. Microbiological Reviews 49:181–212
    [Google Scholar]
  22. Olson M. V. 1991; Genome structure and organization.. In The Molecular and Cellular Biology of the Yeast Saccharomyces pp. 1–39 Edited by Broach J. R., Pringle J. R., Jones E. W. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  23. Petes T. D. 1979a; Meiotic mapping of yeast ribosomal deoxyribo-nucleic acid on chromosome XII.. Journal of Bacteriology 138:185–192
    [Google Scholar]
  24. Petes T. D. 1979b; Yeast ribosomal DNA genes are located on chromosome XII.. Proceedings of the National Academy of Sciences of the United States of America 76:410–414
    [Google Scholar]
  25. Petes T. D. 1980; Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes.. Cell 19:765–774
    [Google Scholar]
  26. Petes T. D., Botstein D. 1977; Simple Mendelian inheritance of the reiterated ribosomal DNA of yeast.. Proceedings of the National Academy of Sciences of the United States of America 745091–5095
    [Google Scholar]
  27. Philippsen P., Thomas M., Kramer R. A., Davis R. W. 1978; Unique arrangement of coding sequences for 5S, 5·8S, 18S and 25S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loop and hybridization analysis.. Journal of Molecular Biology 123:387–401
    [Google Scholar]
  28. Rose M. D., Winston F., Hibter P. 1990 Methods in Yeast Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  29. Schwartz D. C., Cantor C. R. 1984; Separation of yeast chromosome-sized DNAs by pulse field gradient gel electrophoresis.. Cell 37:67–75
    [Google Scholar]
  30. Schweizer E., Mackechnie C., Halvorson H. O. 1969; The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae.. Journal of Molecular Biology 40:261–277
    [Google Scholar]
  31. Selker E. U. 1990; Premeiotic instability of repeated sequences in Neurospora crassa.. Annual Review of Genetics 24:579–613
    [Google Scholar]
  32. Skryabin K. G., Eldarov M. A., Larionov V. L., Bayev A. A., Klootwijk J., De Regt V. C. H. F., Veldman G. M., Planta R. J., Georgiev O. I., Hadjiolov A. A. 1984; Structure and function of the nontranscribed spacer regions of yeast rDNA.. Nucleic Acids Research 12:2955–2968
    [Google Scholar]
  33. Stark G. R., Wahl G. M. 1984; Gene amplification.. Annual Review of Biochemistry 53:447–491
    [Google Scholar]
  34. Stewart S. E., Roeder S. 1989; Transcription by RNA polymerase I stimulates mitotic recombination in Saccharomyces cere- visiae.. Molecular and Cellular Biology 9:3464–3472
    [Google Scholar]
  35. Sugihara K., Ohishi K., Toh-E A. 1986; Ribosomal DNA plasmid isolated from Zygosaccharomyces bailii and its use for constructing yeast vectors effective for intergeneric gene transfer.. Agricultural and Biological Chemistry 50:1503–1512
    [Google Scholar]
  36. Suzuki T., Kobayashi I., Mizuguchi I., Banno I., Tanaka K. 1988; Electrophoretic karyotypes in medically important Candida species.. Journal of General and Applied Microbiology 34:409–416
    [Google Scholar]
  37. Szostak J. W., Wu R. 1980; Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. . Nature, London 284:426–430
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-7-1409
Loading
/content/journal/micro/10.1099/00221287-139-7-1409
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error